2011
DOI: 10.1590/s1516-89132011000500020
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Isolation and screening of microorganisms with potential for biotransformation of terpenic substrates

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Cited by 11 publications
(4 citation statements)
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“…The mycelia were washed with saline water and then re-suspended into the same volume of M9 medium. Limonene (1%, v/v ) was added and cultures were incubated for 6 d. The biomass was filtered and quantified by dry weight to verify the growth of the microorganisms [ 14 ].…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The mycelia were washed with saline water and then re-suspended into the same volume of M9 medium. Limonene (1%, v/v ) was added and cultures were incubated for 6 d. The biomass was filtered and quantified by dry weight to verify the growth of the microorganisms [ 14 ].…”
Section: Methodsmentioning
confidence: 99%
“…Recently, many researches have reported that many strains (fungi, bacteria and yeasts) could yield aroma compounds using limonene as the precursor [ 9 , 14 , 15 , 16 ]. However, the limonene biotransformation process is still limited because of the high volatility and low solubility of this precursor, the low transformation rates, high cytotoxicity of both precursor and product, and so on [ 17 ].…”
Section: Introductionmentioning
confidence: 99%
“…Substrate toxicity was performed to screen monoterpene-resistant microorganisms in accordance with previous methods. 12,13 The culture plates were prepared by displacing 30 ml sterilized NAM in pre-sterilized Petri dishes. Each 1 ml (1.0 x 10 5 CFU/ml) inoculum is evenly distributed to the agar medium with a sterile glass rod.…”
Section: Substrate-toxicity Testmentioning
confidence: 99%
“…To this end, researchers have engineered organisms for its production as well as utilized limonene as a substrate for biotransformation to generate products such as limonene-1,2-diol and α-terpineol (Bier et al., 2017 ; Sales et al., 2018 ; Tai et al., 2016 ). Ongoing studies into the mechanism of limonene toxicity have identified organisms with natural tolerance to limonene (Lennartsson et al., 2012 ) and even some capable of utilizing limonene as a sole carbon source (Bier et al., 2011 ; Sales et al., 2018 ). Likewise, enhanced tolerance to limonene is possible via adaptive laboratory evolution, and rationally engineering genes identified via mutant screening, transporter libraries, and transcriptomics (Chubukov et al., 2015 ; Dunlop et al., 2011 ; Li et al., 2021 ; Ren et al., 2020 ).…”
Section: Carbohydrate-rich Food Wastesmentioning
confidence: 99%