Isolation and replication of mutant populations of wound tumor virions lacking certain genome segments

Evolution and Emergence of Plant Viruses

“…The probability of transmission per transmission event, β p , is mechanistically determined by specific interactions between viral proteins, such as the coat protein or the helper component, and poorly known vector factors, probably of a protein nature (Blanc & Drucker, 2011). It has been repeatedly reported, in different viruses transmitted through different mechanisms, that there is genetic variation in virus transmission factors resulting in the variation of the diminished transmission rate, or in the loss of the ability for vector transmission (e.g., Atreya & Pirone, 1993;Atreya et al, 1991;Bricault & Perry, 2013;Perry & Bricault, 2010;Perry & Francki, 1992;Reddy & Black, 1977;Uyeda et al, 1995). Genetic variation in transmission factors allows speculating that selection could occur on the virus population to optimize the molecular interactions resulting in vector transmission, so that transmission efficiency is increased.…”

“…It is also possible to envision the existence of trade-offs for adaptation to transmission to different vectors, or different transmission mechanisms, which would slow down adaptation to vectors. There is presently no information on this interesting topic, but the frequent loss of vector transmissibility in mechanically passaged viruses (Pirone & Blanc, 1996;Reddy & Black, 1977) suggests the existence of such tradeoffs.…”

Structural properties of double-stranded RNAs associated with biological control of chestnut blight fungus

“…The estimated sizes of WTV segments 1, 6, and 9, previously reported (20), are indicated. The asterisks indicate WTV segments for which lengths were determined by sequence analysis of cDNA clones.…”

Differences in Levels of Detection for the Maize Stripe Virus Capsid and Major Non-capsid Proteins in Plant and Insect Hosts

“…There has been little research on virus-specific proteins, other than capsid proteins, for many of the viruses that infect plant hosts as well as their insect vectors. Wound tumour virus, a member of the reoviridae which infects both plant and insect hosts, has some isolates which can only infect plants (Liu et al, 1975;Reddy & Black, 1977;Nuss & Summers, 1984). These transmissiondefective isolates have deletions in specific genomic RNA segments that are required for infection of insect hosts but not plant hosts.…”