Structural properties of double-stranded RNAs associated with biological control of chestnut blight fungus

Tartaglia, James; Paul, Cynthia P.; Fulbright, D. W.; Nuss, Donald L.

doi:10.1073/pnas.83.23.9109

Cited by 48 publications

(22 citation statements)

References 43 publications

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“…Alternatively, multiple dsRNA molecules can result from subgenomic or defective interfering particles (Tartaglia et al 1986, Hiremath et al 1986). Indeed, the La France S3 dsRNA is an internally deleted variant of the M2 dsRNA (Harmsen et al 1991).…”

Section: Discussionmentioning

confidence: 99%

Double-stranded RNA elements associated with the MVX disease of Agaricus bisporus

Grogan¹,

Adie²,

Gaze³

et al. 2003

Mycological Research

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Double-stranded RNA (dsRNA) has been isolated from Agaricus bisporus fruit bodies exhibiting a wide range of disease symptoms. The symptoms which occurred singularly or in combination included ; bare cropping areas on commercial beds (primordia disruption), crop delay, premature veil opening, off-or brown-coloured mushrooms, sporophore malformations and loss of crop yield. All symptoms were associated with loss of yield and/or product quality. Collectively, these symptoms are described as mushroom virus X (MVX) disease. The dsRNA titre was much lower than that previously encountered with the La France viral disease of mushrooms and a modified cellulose CF11 protocol was used for their detection. A broad survey of cultivated mushrooms from the British industry identified dsRNA elements ranging between 640 bp and 20.2 kbp; the majority have not previously been described in A. bisporus. 26 dsRNA elements were identified with a maximum of 17, apparently non-encapsidated dsRNA elements, in any one sample. Three dsRNAs (16.2, 9.4 and 2.4 kbp) were routinely found in mushrooms asymptomatic for MVX. Previously, La France disease was effectively contained and controlled by minimising the on-farm production and spread of basidiospores. Our on-farm observations suggest that MVX could be spread by infected spores and/or mycelial fragments.

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Section: Discussionmentioning

confidence: 99%

Double-stranded RNA elements associated with the MVX disease of Agaricus bisporus

Grogan¹,

Adie²,

Gaze³

et al. 2003

Mycological Research

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“…The comparative amino acid sequence analysis described above revealed four conserved domains among the gene products of HAV, potyviruses, and BaYMV (one of which is duplicated in HAV). Tartaglia et al (27) For steps iii and iv, the alternative is the loss of the respective function followed by extensive divergence. In view of the potential for movement of the ancestral virus within the mycelium and the frequency of anastomosis, it is conceivable that an extracellular route of infection, and the required packaging function, would have been dispensable.…”

mentioning

confidence: 99%

Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA plant viruses.

Koonin

Choi

Nuss

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

168

119

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Computer-assisted analysis of the putative polypeptide products encoded by the two open reading frames present in a large virus-like double-stranded RNA, L-dsRNA, associated with hypovirulence of the chestnut blight fungus, Cryphonectria parasitica, revealed five distinct domains with significant sequence similarity to previously described conserved domains within plant potyvirus-encoded polyproteins.These included the putative RNA-dependent RNA polymerase, RNA helicase, two papain-like cysteine proteases related to the potyvirus helper-component protease, and a cysteine-rich domain of unknown function similar to the N-terminal portion of the potyvirus helper-component protein. Phylogenetic trees derived from the alignment of the polymerase domains of L-dsRNA, a subset of positive-stranded RNA viruses, and double-stranded RNA viruses, using three independent algorithms, suggested that the hypovirulence-associated dsRNA and potyvirus genomes share a common ancestry. However, comparison of the organization of the conserved domains within the encoded polyproteins of the respective viruses indicated that the proposed subsequent evolution involved extensive genome rearrangement.The phenomenon of transmissible hypovirulence represents a natural form of biological control in which the virulence of Cryphonectria parasitica, the chestnut blight fungus, is modulated by the presence of virus-like genetic elements composed of double-stranded RNA (dsRNA) (reviewed in refs. 1 to 4). Efforts to understand the molecular mechanisms responsible for transmissible hypovirulence have provided an emerging view of the structural and functional properties of hypovirulence-associated dsRNA genetic elements (reviewed in ref. 5). The largest dsRNA present in C. parasitica strain Ep713, L-dsRNA (12,712 base pairs), was shown recently to contain two contiguous coding domains, designated open reading frame (ORF) A and ORF B, consisting of 622 and 3165 codons, respectively (6). Both ORFs encode polyproteins that undergo autocatalytic processing during or immediately after translation (6, 7). On the basis of the similarity of the L-dsRNA genetic organization and expression strategy to those of several viral genomes, it was suggested that L-dsRNA should be considered the equivalent of a viral genome or replicative form and the descriptive term hypovirulence-associated virus (HAV) was proposed (6).Similarities between one of the HAV-encoded proteases, p29, and the potyvirus-encoded protease, HC-Pro, were noted previously (7). In addition, computer-assisted analysis of the C-terminal portion of the ORF B-encoded polyprotein revealed a domain that was clearly related to the RNA helicase of potyviruses (6). We now extend these observations by demonstrating that three additional domains are conserved between the gene products of HAV and those of potyviruses, including the putative RNA polymerase. A detailed analysis of the sequence and organization of the conserved domains within the HAV-encoded and potyvirusencoded polyproteins suggests tha...

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“…Although M 1 and L6, and probably S 1 and $2 d s R N A (Wach et al, 1987), do not seem necessary for expression of the disease, d s R N A s MI, S1 and $2 were never reported to be present in the absence of other major d s R N A segments and thus could be subgenomic. Subgenomic d s R N A s (Field et al, 1983, Tartaglia et al, 1986 and defective interfering d s R N A s (Thiele et al, 1984) occur widely in mycoviruses. Nevertheless, it is unlikely that M1, S1 and $2 represent subgenomic d s R N A s derived from larger d s R N A s because none of these d s R N A s formed homologous hybrids with any of the other d s R N A s (L1 to L5 and M2).…”

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confidence: 99%

Molecular Analysis of Agaricus bisporus Double-stranded RNA

Harmsen

Griensven²,

Wessels

1989

Journal of General Virology

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SUMMARYA detailed analysis was made of dsRNA molecules present in Agarieus bisporus (cultivated mushroom) affected and non-affected by La France disease occurring in The Netherlands. A specific pattern of 10 major dsRNA molecules was always associated with affected strains but non-affected strains also contained at least one of the dsRNAs. Cross-hybridization under stringent conditions showed that the dsRNAs were all unique.

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Structural properties of double-stranded RNAs associated with biological control of chestnut blight fungus

Cited by 48 publications

References 43 publications

Double-stranded RNA elements associated with the MVX disease of Agaricus bisporus

Double-stranded RNA elements associated with the MVX disease of Agaricus bisporus

Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA plant viruses.

Molecular Analysis of Agaricus bisporus Double-stranded RNA

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