Isolation and partial characterization of alginate extracted from Sargassum polycystum collected from three habitats in Banten, Indonesia

Abstract

Abstract. Dharmayanti N, Supriatna J, Abinawanto, Yasman. 2019. Isolation and partial characterization of Alginate from Sargassum polycystum of Banten, Indonesia. Biodiversitas 20: 1776-1785. The utilization of Sargassum polycystum as an alternative alginate source may reduce the dependence on alginate import as currently alginate demands in Indonesia are 100% supplied from overseas. The purpose of this study was to characterize alginate extracted from Sargassum polycystum obtained from three locations with different ecological characteristics. Isolation of alginate was conducted through partial hydrolysis to separate guluronic acid and mannuronic acid followed by freeze-drying and then the parameters were measured qualitatively and quantitatively using FTIR. The results showed that alginate extracted from Sargassum polycystum collected from Lima Island, Ujung Kulon and Binuangeun were 11.48%, 18.62%, and 5.75%, respectively with the viscosity were 35 cP, 62.50 cP, and 81.33 cP, respectively. The test result of partial hydrolysis of alginate showed that guluronic block (GG) in the alginate polymer of Lima Island, Ujung Kulon and Binuangeun were 67.60%, 59.00%, and 41.40%, respectively. These relate to the nature of the gel formed. The alginate from Lima Island tends to be more rigid and less flexible than that from Ujung Kulon and Binuangeun. The findings of this study suggest that there are differences in the concentration of the components of mannuronate and guluronate of Sargassum polycystum across different locations in Banten, Indonesia.

Alginate Profile, Antioxidant, and Antibacterial Activities of Brown Algae <I>Sargassum cristaefolium</I> from Pane Island, North Sumatera

“…Additionally, the proper extraction process, habitat, and lifespan of macroalgae can also affect the yield value. This is in accordance with Dharmayanti et al (2019) who stated that the yield of alginate produced from seaweed is influenced by habitat (light intensity, small wave, or current and aquatic nutrients), the age of brown seaweed, and brown seaweed handling techniques after harvesting and before and the extraction process used. Similarly, according to Sinurat and Marliani (2017), the high yield value in alginate shows that the better the extraction process is carried out and can increase the economic value of alginate.…”

“…While at the base, the moisture content is low because the base has formed guluronic acid and mannuronic acid, which have hydrophilic properties that bind water, increasing the amount of water trapped in guluronic acid and mannuronic acid. According to Dharmayanti et al (2019), the moisture content in alginate is between 5-20%, while the moisture allowed by the FCC ( 2004) is <15%. JECFA also stated that the moisture cotent of food additives of alginate is maximum at 15% (FAO, 2009).…”

The Effect of Alkali Pretreatment and Different Parts of Sargassum polycystum Thallus on Alginic Acid Attributes

“…According to the data, the alginate generated in this study has a viscosity value that is a poor grade. It is lower than previous studies, such as Dharmayanti et al (2019), with 81.33 cP from S. polycystum grown in the western part of Java, but higher than those Anwar et al (2013), with 14.33 to 23.33 cP from S. duplicatum grown in Jepara, Central Java, and only 14.10 cP and 17.22 cP from Caribbean Sargassum and Macrocystis pyrifera, respectively (Mohammed et al, 2020b;Paul et al, 2020). Furthermore, " Draget et al, 1994" produced an alginic acid viscosity of 14.8 cP in the stipe thallus and 14.2 cP in the leaves using Laminaria hyperborea seaweed.…”

“…A review by Hernández-Carmona et al ( 2013) discovered that the commercial-use moisture property criterion must be less than 12%. Other studies revealed that the moisture content of alginate obtained from S. polycystum growth in Banten, Indonesia was 10.23-13.31% (Dharmayanti et al, 2019), S. polycystum growth in Sebesi island was 12.16 %% Sumandiarsa et al (2020), and Sargassum fluitans growth in Alor waters, East Nusa Tenggara was low at 9.35% (Maharani et al, 2017).…”

The effect of Sargassum binderi alginate extract addition on the nutritional content of full cream Mung Bean Milk and its consumer acceptance

“…In Indonesia, seaweed is a commodity that is abundant and easy to find in coastal waters [15]. Alginate is a heteropolysaccharide compound group that has two monomers [17]. The monomers are chain monomers mannuronic acid (poly-Dmannuronic acid) and guluronic acid (poly-Lguluronic acid) [12].…”