Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops

Liu, Huawei; Zhang, Lei; Meng, Aihua; Zhang, Junbiao; Xie, Miaomiao; Qin, Yaohong; Faulk, Dylan Chase; Zhang, Baohong; Yang, Shushen; Qiu, Li

doi:10.1371/journal.pone.0187383

Cited by 23 publications

(18 citation statements)

References 26 publications

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“…Seed-borne pathogens spread and perpetuate bacteria in new plant generations, similarly seeds may also carry beneficial bacteria that may be inherited to new generations. Non-pathogenic seed bacteria have been identified in Phaseolus vulgaris , maize, rice, wheat, alfalfa, and other plants ( Okunishi et al, 2005 ; Rijavec et al, 2007 ; Johnston-Monje and Raizada, 2011 ; Hardoim et al, 2012 ; López-López et al, 2012 ; Liu et al, 2017b ; López et al, 2018 ). Previous analyses of seed endophytes have shown a large diversity of bacteria ( Rijavec et al, 2007 ; Johnston-Monje and Raizada, 2011 ; López-López et al, 2012 ; Rosenblueth et al, 2012 ; Chimwamurombe et al, 2016 ).…”

Section: Sources Of Diazotrophic Bacteriamentioning

confidence: 99%

“…Previous analyses of seed endophytes have shown a large diversity of bacteria ( Rijavec et al, 2007 ; Johnston-Monje and Raizada, 2011 ; López-López et al, 2012 ; Rosenblueth et al, 2012 ; Chimwamurombe et al, 2016 ). Seed isolates from different plants are able to produce auxins, gibberellins, siderophores and ACC deaminase, solubilize phosphates, protect plants against pathogens and fix nitrogen ( Zawoznik et al, 2014 ; Díaz Herrera et al, 2016 ; Khalaf and Raizada, 2016 ; Shahzad et al, 2016 ; Wang et al, 2016 ; Liu et al, 2017b ; Verma et al, 2017 ).…”

Section: Sources Of Diazotrophic Bacteriamentioning

confidence: 99%

“…Klebsiella has been isolated from several distinct plants ( Chelius and Triplett, 2000 ; Martínez et al, 2003 ; Rosenblueth et al, 2004 ; Martínez-Romero et al, 2015 ; Liu et al, 2017b ; Reyna-Flores et al, 2018 ). However, its use in agriculture has been discouraged since some strains of Klebsiella were found to be pathogens or opportunistic pathogens of humans and animals ( Martínez et al, 2004 ; Davidson et al, 2015 ; Martínez-Romero et al, 2018 ).…”

Section: Diazotrophs May Be Human Pathogensmentioning

confidence: 99%

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Nitrogen Fixation in Cereals

et al. 2018

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Cereals such as maize, rice, wheat and sorghum are the most important crops for human nutrition. Like other plants, cereals associate with diverse bacteria (including nitrogen-fixing bacteria called diazotrophs) and fungi. As large amounts of chemical fertilizers are used in cereals, it has always been desirable to promote biological nitrogen fixation in such crops. The quest for nitrogen fixation in cereals started long ago with the isolation of nitrogen-fixing bacteria from different plants. The sources of diazotrophs in cereals may be seeds, soils, and even irrigation water and diazotrophs have been found on roots or as endophytes. Recently, culture-independent molecular approaches have revealed that some rhizobia are found in cereal plants and that bacterial nitrogenase genes are expressed in plants. Since the levels of nitrogen-fixation attained with nitrogen-fixing bacteria in cereals are not high enough to support the plant’s needs and never as good as those obtained with chemical fertilizers or with rhizobium in symbiosis with legumes, it has been the aim of different studies to increase nitrogen-fixation in cereals. In many cases, these efforts have not been successful. However, new diazotroph mutants with enhanced capabilities to excrete ammonium are being successfully used to promote plant growth as commensal bacteria. In addition, there are ambitious projects supported by different funding agencies that are trying to genetically modify maize and other cereals to enhance diazotroph colonization or to fix nitrogen or to form nodules with nitrogen-fixing symbiotic rhizobia.

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Section: Sources Of Diazotrophic Bacteriamentioning

confidence: 99%

Section: Sources Of Diazotrophic Bacteriamentioning

confidence: 99%

Section: Diazotrophs May Be Human Pathogensmentioning

confidence: 99%

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Nitrogen Fixation in Cereals

et al. 2018

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“…Aunque es una técnica específica, es necesario que cuando se utilice se haga la caracterización morfológica y bioquímica de los microorganismos (Liu et al, 2017). Tiene las siguientes ventajas: 1) permite la caracterización ampliada de los microorganismos Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática Gestión y sostenibilidad ambiental e1342 a partir de la identificación del gen nifH; 2) es una prueba rápida y se pueden utilizar kits de extracción de ADN para agilizar el procedimiento, y 3) es altamente sensible en la detección del gel nifH (Leite et al, 2017).…”

Section: Técnicas Pcr Identificación De Los Genes Nifhunclassified

Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática

Fonseca-López

Quila

Balaguera-López

2019

CTA

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La economía del nitrógeno es un tema que viene creciendo a nivel mundial, especialmente en la actividad agrícola cuando por mal manejo de la fertilización ocurren pérdidas por lixiviación que contribuyen a la eutrofización del agua, lo que incrementa la población de algas y, como efecto, reduce la disponibilidad de oxígeno, acelerando el proceso de desnitrificación en el que se produce N2O, conocido por su efecto en el calentamiento global. Igualmente, parte del fertilizante es emitido a la atmósfera por volatización. Esto ha impulsado la creación de técnicas que permiten cuantificar el nitrógeno utilizado por las plantas y el fijado en el suelo a través de microrganismos para hacer más eficiente el empleo del nitrógeno en los sistemas agrícolas. Sin embargo, dentro de las mayores limitantes se encuentra su sensibilidad, especificidad, costo y tecnología que se requiere para aplicarlas. Esto ha conducido a la innovación de procedimientos y a la creación de técnicas que tienen una tasa de error muy bajo. El objetivo de este trabajo fue realizar la descripción de las principales técnicas utilizadas para cuantificar la fijación del nitrógeno con énfasis en los antecedentes, procedimientos, expresiones matemáticas que se usan, y escenarios futuros. La información se describe a partir del análisis de ensayos disponibles en la base de datos Scopus. Este trabajo consolida las técnicas que continúan vigentes para cuantificar el nitrógeno y facilita entender su uso a través del tiempo con modelos de predicción, así como su importancia, ventajas y desventajas.

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“…Alternatively, BNF is an available and ecofriendly way to provide N for plants, which can reduce atmospheric dinitrogen to biologically available ammonium by nitrogen-xing bacteria and archaea. Numerous Diazotrophs could colonize and reproduce in the bulk, rhizosphere soil and rice tissues [4][5][6][7], and increased crop productivity through BNF [8], producing antimicrobials [9], and improving abiotic stress tolerance [10].…”

Section: Introductionmentioning

confidence: 99%

Rice Root-Associated Diazotrophic Community Succession is Driven by Growth Period Combined With Fertilization

Cui¹,

Luo²,

Ye³

et al. 2020

Preprint

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BackgroundBiological nitrogen fixation (BNF) and nitrogenous fertilizers are two crucial ways for nitrogen input in the rice paddy system. Little is known about the effect of nitrogenous fertilizers on root-associated diazotrophs. Here, we investigated the succession of total and active diazotrophs in rhizosphere soil and rice roots under four fertilization treatments (control, NPK, NPK + pig manure, and NPK + rice straw) during three key growth periods (tillering, heading and mature).ResultsThe 15N isotope dilution experiment verified that root-associated diazotrophs could supply N for rice under low nitrogen nutrition by BNF (%Ndfa=11.51). Niche differentiation of diazotrophs existed at the rhizosphere soil-root interface. At both DNA and RNA levels, growth period had stronger effects on the community composition of endophytic diazotrophs than rhizosphere diazotrophs. The Chao1 and Shannon indices of total endophytic diazotrophs dramatically increased at the vegetative stage, and underwent relatively minor changes at the reproductive stage. Furthermore, the community structures of total endophytic diazotrophs were more stabilized in the reproductive stage than the vegetative stage. The number of OTUs shared by rhizosphere soil and roots increased during rice growth. Compared with CK, NPK reduced the relative abundances of Pelobacter and increased the relative abundances of Azoarcus. Pig manure not only improved soil nutrients (OM, TN, TP, AN, AP and NO3-N), but reduced the effect of chemical fertilizers on the community composition of natural rhizosphere diazotrophs. ConclusionsGrowth period demonstrated a stronger influence on the root-associated diazotrophs than fertilization practices. The community structures of total endophytic diazotrophs were more stabilized in the reproductive stage than the vegetative stage, and the alpha-diversity indices and complex of network structures of endophytic diazotrophs increased at the vegetative stage. Replacing chemical fertilization with pig manure not only increased soil nutrients, but regulated rhizosphere diazotrophic community structures. Understanding the combined effects of growth period and fertilization on root-associated diazotrophs presents the basis towards the sustainable crop production.

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Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops

Cited by 23 publications

References 26 publications

Nitrogen Fixation in Cereals

Nitrogen Fixation in Cereals

Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática

Rice Root-Associated Diazotrophic Community Succession is Driven by Growth Period Combined With Fertilization

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