Isolation and molecular identification of Lumpy Skin Disease (LSD) virus from infected cattle in Bangladesh

Giasuddin, M; Yousuf, MA; Hasan, Mahmudul; Rahman, MH; Hassan, Md. Rakibul; Ali, Md. Yousuf

doi:10.3329/bjlr.v26i1-2.49933

Cited by 10 publications

(10 citation statements)

References 9 publications

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“…In Bangladesh, an outbreak of an unknown syndrome with nodular skin lesions was reported by local veterinary services authority in mid‐2019 in commercial and backyard cattle population in some locations (Anwara, Karnaphuli and Patiya) of Chattogram district (Anonymous, 2019). Same pattern of clinical onset was reported later in different districts of the country (Giasuddin et al., 2020; Khalil et al., 2021). The outbreak report was preliminary confirmed based on clinical signs and later using the reverse transcription polymerase chain reaction (RT‐PCR) test by the Department of Livestock Services (DLS), Bangladesh and notified the disease as LSD to OIE in August, 2019 (Anonymous, 2019).…”

Section: Introductionsupporting

confidence: 71%

Lumpy skin disease outbreak in cattle population of Chattogram, Bangladesh

Hasib

Islam

Das

et al. 2021

Veterinary Medicine & Sci

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Background Lumpy skin disease (LSD) is an important viral disease causing significant economic losses in commercial livestock production. In mid‐2019, an outbreak of LSD has been reported in cattle population from different parts of Bangladesh including Chattogram division. A cross‐sectional surveillance study was undertaken from August 2019 to December 2019 to investigate the prevalence and associated risk factors of LSD in cattle in Chattogram district. Methods A total of 3,327 cattle from 19 commercial farms were examined for the LSD specific skin lesions and associated risk factors. A total of 120 skin biopsies were collected from the suspected animal for the confirmation of the disease using molecular detection and histopathological examination. Partial genome sequencing and phylogenetic analyses were performed on selected viral isolates. Results The overall clinical prevalence of LSD in the study population was 10% (95% confidence interval [CI]: 9.4%–11%) where the highest farm level outbreak frequency was 63.33% (95% CI: 45.51%–78.13%) and the lowest 4.22% (95% CI: 3.39%–5.25%). Crossbred and female cattle showed a significantly higher prevalence of the disease compared to their counterparts. Introduction of new animals in farms was found to be one of the most significant risk factors in the transmission of the disease. All suspected skin biopsies were positive for LSD virus (LSDV) infection with granulomatous and pyogranulomatous dermatitis was revealed on histopathology. Phylogenetic analysis based on the inverted terminal repeat region of the LSDV gene suggested that the locally circulating strain was closely related to the strains isolated from the Middle East and North African countries. Conclusions The data generated in this study would be beneficial to the field veterinarians and animal health decision makers in the country as well as it will aid in taking appropriate measures to prevent further relapse or outbreak of this disease in future.

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Section: Introductionsupporting

confidence: 71%

Lumpy skin disease outbreak in cattle population of Chattogram, Bangladesh

Hasib

Islam

Das

et al. 2021

Veterinary Medicine & Sci

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“…In 2019, the prevalence of LSD was 10.00% in Bangladesh [ 51 ], 19.50% in China [ 52 ], 22.28%–27.50% in Egypt [ 36 , 53 ], and 37.66% in India [ 54 ]. In 2020, the prevalence of LSD was 3.00%–6.00% in Myanmar [ 55 ], 4.85% and 53.20% in Nepal [ 56 , 57 ], 13.93% in India [ 58 ], and 78.00% in Bangladesh [ 59 ]. In 2021–2022, the prevalence of LSD was 70% in Egypt [ 60 ], 4.17% in Thailand [ 61 ], 5.9% in Mongolia [ 62 ], and 36.2% in Ethiopia [ 63 ].…”

Section: Epidemiology (Susceptibility Hosts Prevalence In Other Count...mentioning

confidence: 99%

Lumpy skin disease: A newly emerging disease in Southeast Asia

2022

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Lumpy skin disease (LSD) is caused by LSD virus (LSDV). This virus has been classified in the genus Capripoxvirus, family Poxviridae which generally affects large ruminants, especially cattle and domestic water buffalo. The first outbreak of LSD was found in 1929 in Zambia, then spreading throughout Africa and with an ongoing expanding distribution to Asia and Europe. In 2020, LSD was found from Southeast Asia in Vietnam and Myanmar before reaching Thailand and Laos in 2021. Therefore, LSD is a newly emerging disease that occurs in Southeast Asia and needs more research about pathology, transmission, diagnosis, distribution, prevention, and control. The results from this review show the nature of LSD, distribution, and epidemic maps which are helpful for further information on the control and prevention of LSD.

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“…In Bangladesh, the first outbreaks of LSD were reported in commercial and backyard holdings in the Chattogram district [31]. Further outbreaks were later reported throughout the country [32][33][34]. Currently, LSD is one of the most economically important emerging livestock diseases in Bangladesh due to its wide distribution and huge cattle population in the backyard or household settings, which add to the increasing poverty and decreasing food security [32].…”

Section: Introductionmentioning

confidence: 99%

Clinical Epidemiology, Pathology, and Molecular Investigation of Lumpy Skin Disease Outbreaks in Bangladesh during 2020–2021 Indicate the Re-Emergence of an Old African Strain

Parvin

Chowdhury

Islam

et al. 2022

Viruses

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Lumpy skin disease (LSD) emerged in Bangladesh in mid-2019, leading to great economic losses for cattle farmers. This study describes the recent occurrence of the LSDV in Bangladesh and examines the clinical manifestation of the disease in local cattle breeds, characteristic epidemiological features, and pathological findings in affected animals. In addition, a full-genome sequencing of two local LSDV isolates was carried out. A total of 565 animals from 88 households were investigated, and 165 samples (skin lesions, saliva, nasal discharge, feces, and milk) were collected for virus detection. Pathology and immunohistochemistry were performed on nodule biopsies. Fever, nodular skin lesions, and swelling of the joints were the most common clinical manifestations. Skin lesions had a higher concentration of viral DNA compared to other sample types and were therefore selected for virus isolation and characterization. Pathology of the LSD skin nodules comprised a granulomatous reaction in the dermis and hypodermis that extended to the surrounding tissues. Development of the skin lesions started with swelling of keratinocytes with cytoplasmic vacuolation, vasculitis, panniculitis, thrombosis, and infarction. Altogether, the LSDV produced transmural, hemorrhagic, necrotizing, proliferative and ulcerative dermatitis. The LSD viral antigen was detected occasionally in the macrophages, epithelial cells, and vascular smooth muscle cells. The complete genome sequence analysis revealed that the two Bangladeshi field strains (BD-V392.1 and BD-V395.1) were distinct from the contemporary field strains and were closely related to the ancestral African Neethling strain. The findings of this study will improve the diagnosis, monitoring, and control of LSD in Bangladesh.

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Isolation and molecular identification of Lumpy Skin Disease (LSD) virus from infected cattle in Bangladesh

Cited by 10 publications

References 9 publications

Lumpy skin disease outbreak in cattle population of Chattogram, Bangladesh

Lumpy skin disease outbreak in cattle population of Chattogram, Bangladesh

Lumpy skin disease: A newly emerging disease in Southeast Asia

Clinical Epidemiology, Pathology, and Molecular Investigation of Lumpy Skin Disease Outbreaks in Bangladesh during 2020–2021 Indicate the Re-Emergence of an Old African Strain

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