Isolation and molecular characterization of Acanthamoeba from patients with keratitis in Spain

Martín-Pérez, Tania; Criado–Fornelio, A.; Martı́nez, Javier; Blanco, A I de Cos; Fuentes, Isabel; Pérez-Serrano, Jorge

doi:10.1016/j.ejop.2017.06.009

Cited by 26 publications

(23 citation statements)

References 49 publications

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“…The result of genotyping analysis was consistent with our previous study [9] and other study in China [26] . Although the subtypes were different, the genotype T4 is still the main AK-related genotype in China, furthermore, genotype T4 is the predominant genotype in other countries and areas [27][28][29][30] .…”

Section: Discussionmentioning

confidence: 99%

Acanthamoeba keratitis related to contact lens use in a tertiary hospital in China

Wang

et al. 2019

Preprint

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Background: To report the clinical and microbiological features of Acanthamoeba keratitis (AK) related to contact lens use in a tertiary hospital in China.Methods: In this retrospective study, the medical results of 61 cases of AK related to contact lens use from January 2000 to December 2017 were reviewed. The data included patients' demographics, lens type, history, risk factors, disease stages, corneal scraping and culture reports, and treatments.Moreover, genotypic identification of some of the isolates was carried out with a PCR assay and sequence analysis of the 18S ribosomal DNA gene.Results: There were 64 eyes included in the study. A total of 32.8% of the patients wore soft contact lenses, and 67.2% of patients used overnight orthokeratology. In the cases (20 eyes) in the early stage, 65% (13 eyes) had positive results according to Giemsa-stained smears, and 0.9% sodium chloride (NaCl) wet mounts revealed trophozoites in 7 eyes (35%). Six eyes (30%) were diagnosed by confocal microscopy combined with clinical signs. In the orthokeratology patients, 87.8% (36/41)

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Section: Discussionmentioning

confidence: 99%

Acanthamoeba keratitis related to contact lens use in a tertiary hospital in China

Wang

et al. 2019

Preprint

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“…T4 Neff appeared sister to either T4 A/B or T4 D/E mixed clades. T4 C/F clade always branched after T4 D/E , except for the phylogeny using GTR model, where T4 D was robustly sister to T4 A/B/Neff cluster (Martín-Pérez et al, 2017).…”

Section: Smentioning

confidence: 97%

“…The distinctiveness of different clades inside type T4 has begun to manifest itself since the first works on Acanthamoeba rDNA diversity (Gast et al , 1996;Stothard et al , 1998;Chung et al , 1998;Booton et al , 2002;Kong, 2009;Fuerst et al , 2015;Martín-Pérez et al , 2017). Subtypes T4 C , T4 D , T4 E , T4 F , and T4 Neff , when represented by several sequences, were usually well-supported, while T4 B was sometimes mixed with T4 A , and the latter mostly paraphyletic.…”

Section: Smentioning

confidence: 99%

Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)

Malavin

Shmakova

2019

Preprint

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Acanthamoeba castellanii species complex (genotype T4) comprises of more than ten species with unclear synonymy, as molecular phylogeny has several conflicts with published morphological data. In this paper, we analyze quantitative traits and temperature preferences in six new strains belonging to A. castellanii complex isolated from Arctic permafrost in the framework of molecular phylogeny. This integrative approach allows us to cross-link genotypic and phenotypic variability and identify species-level boundaries inside the complex. We also analyze previously known and newly identified discrepancies between the nuclear and mitochondrial gene-based phylogenies. We hypothesize that one reason for these discrepancies may be the intragenomic polymorphism of ribosomal RNA genes.

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“…or in case of corneal trauma. AK is mainly caused by isolates with T4 genotype [8][9][10]; however, T2, T3, T5, T6, T8, T9, T11, T13, and T15 genotype species have also been identified in patients with AK, as shown in Table I [11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Clinical course of Acanthamoeba keratitis by genotypes T4 and T8 in Hungary

Orosz

Kriskó

Shi

et al. 2019

AMicr

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Genus Acanthamoeba is an opportunistic protozoan that is widely distributed in the environment. Within this genus, numerous species are recognized as human pathogens, potentially causing Acanthamoeba keratitis (AK). AK is a corneal disease, associated predominantly with contact lens (CL) wear; its epidemiology is related to the specific Acanthamoeba genotypes. This study reports seven CL wearer, Acanthamoeba PCR-positive patients with AK, diagnosed between January 2015 and 2018. Patients had the diagnosis of AK 1.36 months after first symptoms. Genotyping allowed the identification of six isolates of the T4 and one of the T8 genotypes. At first presentation, pseudendritiformic epithelopathy/dirty epithelium (four eyes, 57.1%), multifocal stromal infiltrates (five eyes, 71.4%), ring infiltrate (three eyes, 42.8%), and perineuritis (one eye, 14.3%) were observed. AK was healed without later recurrence in two eyes (28.5%) using triple-topical therapy, in three eyes (42.8%) following additional penetrating keratoplasty. In one patient (14.3%), AK recurred following successful application of triple-therapy and was treated successfully with repeated triple-topical therapy and in one patient (14.3%), no follow-up data were available after diagnosis. We could not observe correlation of genotype and clinical course or the necessity of corneal transplantation in our case series.

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Isolation and molecular characterization of Acanthamoeba from patients with keratitis in Spain

Cited by 26 publications

References 49 publications

Acanthamoeba keratitis related to contact lens use in a tertiary hospital in China

Acanthamoeba keratitis related to contact lens use in a tertiary hospital in China

Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)

Clinical course of Acanthamoeba keratitis by genotypes T4 and T8 in Hungary

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