Isolation and molecular characterization of Corynebacterium pseudotuberculosis from sheep and goats in Mexico

Guerrero, Jorge Antonio Varela; Jiménez, Roberto Montes de Oca; Acosta-Dibarrat, Jorge; León, Fernando Hernández; Morales-Erasto, Vladimir; Salazar, Humberto Gustavo Monroy

doi:10.1016/j.micpath.2018.02.031

Cited by 17 publications

(15 citation statements)

References 19 publications

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“…The frequent isolation of C. pseudotuberculosis from the MTLNs indicates that this pathogen can have influence on small ruminant health, which had been confirmed in previous reports [32,33]. In the last few decades there has been an increasing number of caseous lymphadenitis (CLA) cases, caused by C. pseudotuberculosis, in sheep and goat flocks in many regions of the world [9,34,35]. CLA is manifested as an abscesses containing white-yellow dense pus often with mineralization.…”

Section: Discussionmentioning

confidence: 59%

Microbiological assessment of sheep lymph nodes with lymphadenitis found during post-mortem examination of slaughtered sheep: implications for veterinary-sanitary meat control

et al. 2020

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Background: Microbiological examination of lesions found in slaughtered animals during meat inspection is an important part of public health protection as such lesions may be due to zoonotic agents that can be transmitted by meat. Examination of inflamed lymph nodes also plays a particular important role, as lymphadenitis may reflect a more widespread infection. Such lesions in sheep are mainly caused by pyogenic bacteria but also mycobacteria are occasionally found. Meat inspection data from 2017 to 2018 from southern Poland, especially from the Małopolska region, indicate that purulent or caseous lymphadenitis involving the mediastinal and tracheobronchial lymph nodes (MTLNs) is a common finding. The primary aim of the current study was to determine the aetiology of these lesions. Furthermore, it was investigated how presence of lesions was correlated with age and grazing strategy of affected sheep. Results: Post-mortem examination revealed purulent or caseous lymphadenitis in the MTLNs of 49 out of 284 animals (17.3%). Subsequent microbiological examination revealed the presence of Corynebacterium pseudotuberculosis (34.7%), Streptococcus dysgalactiae subsp. equisimilis (34.7%), Staphylococcus aureus (8.2%), Enterococcus spp. (2.0%), Trueperella pyogenes (2.0%), and β-haemolytic strains of Escherichia coli (2.0%). Mycobacterium spp. and Rhodococcus equi were not detected. In older sheep, the probability of the presence of purulent or caseous lymphadenitis was higher than in younger, and the risk was increasing by 1.5% with each month of life. Sheep grazing locally had 4.5times greater risk of having purulent or caseous lymphadenitis than individuals summer grazing in the mountains. Conclusion: The most common aetiological agents of purulent or caseous lymphadenitis in the MTLNs of sheep in the Małopolska region were C. pseudotuberculosis and S. dysgalactiae subsp. equisimilis. Particular attention during post-mortem examination should be paid to the carcasses of older sheep and sheep grazing on permanent pastures, as they seem more prone to develop purulent or caseous lymphadenitis.

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Section: Discussionmentioning

confidence: 59%

Microbiological assessment of sheep lymph nodes with lymphadenitis found during post-mortem examination of slaughtered sheep: implications for veterinary-sanitary meat control

et al. 2020

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“…It is obvious from phylogenetic analysis, the 16S rRNA gene sequence could only be useful to separate Corynebacterium pseudotuberculosis from other Corynebacterium spp., but not to differentiate biovar ovis from biovar equi [ 36 , 37 ]. This differentiation step could further be done using fusA [ 36 ] or rpoB gene [ 50 ]. The close phenotypic and genotypic relationship among Corynebacterium pseudotuberculosis isolates from various countries in the world and among different animal species, might be evidence that the infections had a common source [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020

Terab¹,

Wahab²,

Ishag³

et al. 2021

PLoS ONE

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Caseous lymphadenitis (CLA) or pseudotuberculosis is a chronic zoonotic bacterial disease caused by Corynebacterium pseudotuberculosis, which affects livestock and humans. This study aimed to describe the pathology, bacteriology and confirm the identity of the pathogen by 16S rRNA gene sequencing in Camelus dromedarius. A total of 12 camels with suspected CLA in three regions of Abu Dhabi Emirate (Abu Dhabi, Al Ain and Al Dhafra), United Arab Emirate (UAE) were subjected to clinical and postmortem examinations from January 2015 to December 2020. Clinically, camels were emaciated and showed the presence of external caseous abscesses suggestive of CLA. Postmortem examination showed multiple abscesses of variable sizes with caseous material encapsulated by fibrous tissue in the liver, lungs, muscle, and lymph nodes. Following clinical and postmortem examination, blood, pus and different tissue samples were collected for subsequent analysis. Histopathological examination of all organs stained with Hematoxylin and Eosin (H&E) indicated a central caseo-necrotic core that was admixed with bacterial colonies and infiltration of chronic inflammatory cells, surrounded by a pyogenic membrane, and an outer fibrous connective tissue capsule. Bacterial culture identified the isolates of Corynebacterium pseudotuberculosis biotype ovis strain, and these isolates were shown to be sensitive to all antibiotics tested (penicillin, ampicillin, Co-trimoxazole, enrofloxacin and tetracycline). Moreover, the identity of the isolates was confirmed by partial sequencing of the 16S rRNA gene which showed a 100% identity to Corynebacterium pseudotuberculosis. Phylogenetic analysis based on 16S rRNA gene sequence clearly differentiates Corynebacterium pseudotuberculosis from other species of Corynebacterium. Briefly, this study provided the basic information for infection of Corynebacterium pseudotuberculosis in Camels and will help in controlling of this pathogen in the region.

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“…At present, molecular methods, e.g., PCR, genome sequencing, and MALDI-TOF MS enable definitive diagnosis, along with geographical distribution studies, virulence profile, and genetic diversity investigation of Corynebacterium species from domestic animals worldwide, including C. pseudotuberculosis strains [2,7,8,[31][32][33][34]. In the coming years, large-scale sequencing has seen used to investigate the microbiota of lymph nodes from humans [35] and animals (pigs) [36], with the aim to diagnose and differentiate agents/diseases; however, this next generation sequencing method has not yet been applied in the investigation of lymph nodes from sheep with and without lesions compatible with CL.…”

Section: Discussionmentioning

confidence: 99%

Bacteriological, cytological, and molecular investigation of Corynebacterium pseudotuberculosis, mycobacteria, and other bacteria in caseous lymphadenitis and healthy lymph nodes of slaughtered sheep

et al. 2020

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Isolation and molecular characterization of Corynebacterium pseudotuberculosis from sheep and goats in Mexico

Cited by 17 publications

References 19 publications

Microbiological assessment of sheep lymph nodes with lymphadenitis found during post-mortem examination of slaughtered sheep: implications for veterinary-sanitary meat control

Microbiological assessment of sheep lymph nodes with lymphadenitis found during post-mortem examination of slaughtered sheep: implications for veterinary-sanitary meat control

Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020

Bacteriological, cytological, and molecular investigation of Corynebacterium pseudotuberculosis, mycobacteria, and other bacteria in caseous lymphadenitis and healthy lymph nodes of slaughtered sheep

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