Bacteriological, cytological, and molecular investigation of Corynebacterium pseudotuberculosis, mycobacteria, and other bacteria in caseous lymphadenitis and healthy lymph nodes of slaughtered sheep

Zamprogna, Thiago de Oliveira; Ribeiro, Débora da Silva Freitas; Azevedo, Vasco; Lara, Gustavo Henrique Batista; Motta, Rodrigo Garcia; Silva, Rodrigo da; Siqueira, Amanda Keller; Júnior, Geraldo de Nardi; Listoni, Fernando José Paganini; Martins, Lorrayne de Souza Araújo; Silva, Aristeu Vieira da; Portilho, Fábio Vinícius Ramos; Mota, André da Rocha; Rodrigues, Carolina Aparecida; Almeida, Beatriz; Ribeiro, Márcio Garcia

doi:10.1007/s42770-020-00403-0

Cited by 6 publications

(2 citation statements)

References 32 publications

(92 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also known as pseudotuberculosis or “cheesy gland.” The disease is caused by a Gram-positive facultative intracellular parasite called Corynebacterium pseudotuberculosis ( Cp ). It belongs to the vitally important CMNR group encompassing Corynebacterium , Mycobacterium , Nocardia , and Rhodococcus [ 1 , 2 , 3 ]. It is often referred to as “iceberg disease” because it can cause a gradual decline in physical condition, thin ewe syndrome, fertility problems, behavioral changes, decreased milk and wool production, and poor carcass quality [ 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Kyselová

Tichý

Sztankóová

et al. 2023

Animals

View full text Add to dashboard Cite

Caseous lymphadenitis (CL) is a chronic contagious disease that affects small ruminants and is characterized by the formation of pyogranulomas in lymph nodes and other organs. However, the pathogenesis of this disease and the response of the host genome to infection are not yet fully understood. This study aimed to investigate the whole blood transcriptome and evaluate differential gene expression during the later stages of CL in naturally infected ewes. The study included diseased, serologically positive (EP), exposed, serologically negative (EN) ewes from the same infected flock and healthy ewes (CN) from a different flock. RNA sequencing was performed using the Illumina NextSeq system, and differential gene expression was estimated using DESeq2 and Edge R approaches. The analysis identified 191 annotated differentially expressed genes (DEGs) in the EP group (102 upregulated and 89 downregulated) and 256 DEGs in the EN group (106 upregulated and 150 downregulated) compared to the CN group. Numerous immunoregulatory interactions between lymphoid and nonlymphoid cells were influenced in both EP and EN ewes. Immune DEGs were preferentially assigned to antigen presentation through the MHC complex, T lymphocyte-mediated immunity, and extracellular matrix interactions. Furthermore, the EP group showed altered regulation of cytokine and chemokine signaling and activation and recombination of B-cell receptors. Conversely, NF-kappa B signaling, apoptosis, and stress response were the main processes influenced in the EN group. In addition, statistically significant enrichment of the essential immune pathways of binding and uptake of ligands by scavenger receptors in EP and p53 signaling in the EN group was found. In conclusion, this study provides new insights into the disease course and host–pathogen interaction in naturally CL-infected sheep by investigating the blood transcriptome.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Kyselová

Tichý

Sztankóová

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

“…The disease is caused by a Gram-positive facultative intracellular parasite called Corynebacterium pseudotuberculosis (Cp). It belongs to the vitally important CMNR group encompassing Corynebacterium, Mycobacterium, Nocardia, and Rhodococcus [1][2][3]. It is often referred to as "iceberg disease" because it can cause a gradual decline in physical condition, thin ewe syndrome, fertility problems, behavioral changes, decreased milk and wool production, and poor carcass quality [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Immune Response in Sheep With Caseous Lymphadenitis Through Analysis of the Whole Blood Transcriptome

Kyselová¹,

Tichý²,

Sztankóová³

et al. 2023

Preprint

View full text Add to dashboard Cite

Caseous lymphadenitis (CL) is a chronic contagious disease that affects small ruminants and is characterized by the formation of pyogranulomas in lymph nodes and other organs. However, the pathogenesis of this disease and the response of the host genome to infection are not yet fully understood. This study aimed to investigate the whole blood transcriptome and evaluate differential gene expression during the later stages of CL in naturally infected ewes. The study included diseased, serologically positive (EP), exposed, serologically negative (EN) ewes from the same infected flock and healthy ewes (CN) from a different flock. RNA sequencing was performed using the Illumina NextSeq system, and differential gene expression was estimated using DESeq2 and Edge R approaches. The analysis identified 191 annotated differentially expressed genes (DEGs) in the EP group (102 upregulated and 89 downregulated) and 256 DEGs in the EN group (106 upregulated and 150 downregulated). Infection influenced numerous immunoregulatory interactions between lymphoid and nonlymphoid cells in both EP and EN ewes. Immune DEGs were preferentially assigned to antigen presentation through the MHC complex, T-lymphocyte mediated immunity, and extracellular matrix interactions. Furthermore, the EP group showed altered regulation of cytokine and chemokine signalling and activation and recombination of B-cell receptors. Conversely, NF-kappa B signalling, apoptosis, and stress response were the main processes influenced in the EN group. In addition, statistically significant enrichment of the essential immune pathways of Binding and Uptake of Ligands by Scavenger Receptors in EP and p53 signalling in the EN group was found. In conclusion, this study provides new insights into the disease course and host‒pathogen interaction in naturally CL-infected sheep by investigating the blood transcriptome.

show abstract

A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats

Nicoleti

Braga

Stanisić

et al. 2023

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Bacteriological, cytological, and molecular investigation of Corynebacterium pseudotuberculosis, mycobacteria, and other bacteria in caseous lymphadenitis and healthy lymph nodes of slaughtered sheep

Cited by 6 publications

References 32 publications

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Comparative Characterization of Immune Response in Sheep With Caseous Lymphadenitis Through Analysis of the Whole Blood Transcriptome

A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats

Contact Info

Product

Resources

About