Isolation and initial characterization of human glioblastoma cells resistant to photodynamic therapy

Vilchez, María Laura; Rodríguez, Lorena; Palacios, Rodrigo E.; Prucca, César G.; Caverzán, Matías Daniel; Caputto, Beatriz L.; Rivarola, Viviana; Sanabria, Laura Natalia Milla

doi:10.1016/j.pdpdt.2020.102097

Cited by 9 publications

(8 citation statements)

References 30 publications

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“…Therefore, detailed characterization of tumor cells and their degree of sensitivity to PDT is urgently needed. Several in vitro and in vivo approaches for isolation and selection of PDT-resistant tumor cells are known [ 188 , 189 ]. These models can be used for detailed study of cell morphology and population characteristics, molecular mechanisms of sensitivity to PSs (based on inherent and induced resistance in different cell lines), and metastatic abilities of tumor cells in mice.…”

Section: Ferroptosismentioning

confidence: 99%

Which cell death modality wins the contest for photodynamic therapy of cancer?

et al. 2022

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Photodynamic therapy (PDT) was discovered more than 100 years ago. Since then, many protocols and agents for PDT have been proposed for the treatment of several types of cancer. Traditionally, cell death induced by PDT was categorized into three types: apoptosis, cell death associated with autophagy, and necrosis. However, with the discovery of several other regulated cell death modalities in recent years, it has become clear that this is a rather simple understanding of the mechanisms of action of PDT. New observations revealed that cancer cells exposed to PDT can pass through various non-conventional cell death pathways, such as paraptosis, parthanatos, mitotic catastrophe, pyroptosis, necroptosis, and ferroptosis. Nowadays, immunogenic cell death (ICD) has become one of the most promising ways to eradicate tumor cells by activation of the T-cell adaptive immune response and induction of long-term immunological memory. ICD can be triggered by many anti-cancer treatment methods, including PDT. In this review, we critically discuss recent findings on the non-conventional cell death mechanisms triggered by PDT. Next, we emphasize the role and contribution of ICD in these PDT-induced non-conventional cell death modalities. Finally, we discuss the obstacles and propose several areas of research that will help to overcome these challenges and lead to the development of highly effective anti-cancer therapy based on PDT.

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Section: Ferroptosismentioning

confidence: 99%

Which cell death modality wins the contest for photodynamic therapy of cancer?

et al. 2022

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“…We found that PDT resistant GBM cells had higher proliferation capacity in 3D cultures, more tumorigenic capacity and higher mRNA levels of growth factor receptors fibroblast growth factor receptor (FGFR), EGFR and βPDGFR than parental cells. Furthermore, they accumulate less photosensitizer PpIX when they are incubated with Me-ALA, compared to the parental population (Vilchez et al, 2021). This could cause lower has been associated with certain DAMPs and it has been observed that PS localization at subcellular level was important in ICD, especially the endoplasmic reticulum (ER) (Rodríguez et al, 2016).…”

Section: Oxidative S Tre Ss In G B M and Its Impac T In Pdt Effi C mentioning

confidence: 99%

Understanding the glioblastoma tumor biology to optimize photodynamic therapy: From molecular to cellular events

Ibarra

Vilchez

Caverzán

et al. 2020

J of Neuroscience Research

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Gliomas are malignant tumors that originate in the central nervous system (CNS). Any tumor that forms in glial cells, or in supporting tissue of the brain is called a glioma. Glioblastoma (GBM) is a subtype of glioma that tends to grow rapidly, spread to other tissues, and has a poor prognosis. GBM is the most aggressive and the most common adult primary intracranial neoplasm (Delgado-Martín & Medina, 2020; Louis et al., 2016). GBM is more common in people ages 50 to 70 and is more prevalent in men than women (Louis et al., 2016). The symptoms or signs of the presence of this type of tumor are: increased pressure on the brain, headaches, seizures, memory loss, and behavioral changes (Sizoo et al., 2010). GBM was previously known as glioblastoma multiforme and the term "multiform" refers to the tumor heterogeneity (Sturm et al., 2014). GBMs may have originated from different kinds of cells, such as astrocytes and oligodendrocytes. The histological features that distinguish GBM from all the other gliomas are the presence of necrosis (dead cells) and the increase in blood vessels around the tumor (D'Alessio et al., 2019). The World Health

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“…In this case, the surviving cells may have higher resistance and malignancy than the initial parental cells. The inability to suffer death after PDT treatment means a selective advantage in tumor progression (Vilchez et al, 2021). Exposure to repeated cycles of PDT is a robust selection process that allows only the most resistant cells to survive (Ghahe et al, 2021;Vilchez et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…The inability to suffer death after PDT treatment means a selective advantage in tumor progression (Vilchez et al, 2021). Exposure to repeated cycles of PDT is a robust selection process that allows only the most resistant cells to survive (Ghahe et al, 2021;Vilchez et al, 2021). Research has shown that repetitive ALA-mediated PDT can isolate resistant murine adenocarcinoma for the mechanistic setting of new, potential targets and the development of cancer resistance novel therapeutic strategies (Casas et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Characterization of resistant MCF-7 breast cancer cells developed by repeated cycles of photodynamic therapy

2022

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Breast cancer mainly affects women and causes a severe global threat to health. It is often managed and treated with surgery, chemotherapy, immunotherapy, and radiation therapy. Generally, chemotherapy as a treatment option is often opposed by responsive tumor relapse and development of resistance, a significant setback of current treatment. Photodynamic therapy (PDT) offers a promising modality that can treat cancer by combining a photosensitizer and laser irradiation in the presence of oxygen. However, one problem of PDT in treating breast cancer is the apparition of the resistant cell population. Thus, we aimed for stepwise selection and characterization of MCF-7 cells resistant to PDT with a sulfonated zinc phthalocyanine (ZnPcS4) photosensitizer. The wild-type MCF-7 was exposed to successive cycles of ZnPcS4-PDT, and 10resistant populations were finally obtained. In wild-type and parental cells, we analyzed the cell morphology (light microscopy), cell cycle (BrdU staining), cell viability (MTT assay), antioxidant activity (superoxide dismutase measurement), and immunofluorescence expression of resistant p-glycoprotein (P-gp). The results indicate that resistant cells showed a mesenchymal cell phenotype, few differences in antioxidant activity, an increased DNA synthesis, and more expression of P-gp than the wild-type parental cells. These distinctive features of resistant cells can provide insight into the emergence of MCF-7 cell resistance to PDT, which was necessary to design the best therapeutic procedure for improved efficacy.

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Isolation and initial characterization of human glioblastoma cells resistant to photodynamic therapy

Cited by 9 publications

References 30 publications

Which cell death modality wins the contest for photodynamic therapy of cancer?

Which cell death modality wins the contest for photodynamic therapy of cancer?

Understanding the glioblastoma tumor biology to optimize photodynamic therapy: From molecular to cellular events

Characterization of resistant MCF-7 breast cancer cells developed by repeated cycles of photodynamic therapy

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