Isolation and identification of halotolerant soil bacteria from coastal Patenga area

Rahman, Shafkat Shamim; Siddique, Romana; Tabassum, Nafisa

doi:10.1186/s13104-017-2855-7

Cited by 32 publications

(22 citation statements)

References 20 publications

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“…The plates were inoculated with fixed volume of inoculum (OD = 0.05) incubated at 30˚C for 7 days. The LB plates with 1% NaCl (w/v) were used as control [13].…”

Section: Isolation Of Rhizobacteria and Halo-tolerance Assaymentioning

confidence: 99%

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

et al. 2020

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Salt stress is one of the devastating factors that hampers growth and productivity of soybean. Use of Pseudomonas pseudoalcaligenes to improve salt tolerance in soybean has not been thoroughly explored yet. Therefore, we observed the response of hydroponically grown soybean plants, inoculated with halotolerant P. pseudoalcaligenes (SRM-16) and Bacillus subtilis (SRM-3) under salt stress. In vitro testing of 44 bacterial isolates revealed that four isolates showed high salt tolerance. Among them, B. subtilis and P. pseudoalcaligenes showed ACC deaminase activity, siderophore and indole acetic acid (IAA) production and were selected for the current study. We determined that 10 6 cells/mL of B. subtilis and P. pseudoalcaligenes was sufficient to induce tolerance in soybean against salinity stress (100 mM NaCl) in hydroponics by enhancing plant biomass, relative water content and osmolytes. Upon exposure of salinity stress, P. pseudoalcaligenes inoculated soybean plants showed tolerance by the increased activities of defense related system such as ion transport, antioxidant enzymes, proline and MDA content in shoots and roots. The Na + concentration in the soybean plants was increased in the salt stress; while, bacterial priming significantly reduced the Na + concentration in the salt stressed soybean plants. However, the antagonistic results were observed for K + concentration. Additionally, soybean primed with P. pseudoalcaligenes and exposed to 100 mM NaCl showed a new protein band of 28 kDa suggesting that P. pseudoalcaligenes effectively reduced salt stress. Our results showed that salinity tolerance was more pronounced in P. pseudoalcaligenes as compared to B. subtilis. However, a detailed study at molecular level to interpret the mechanism by which P. pseudoalcaligenes alleviates salt stress in soybean plants need to be explored.

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“…The plates were inoculated with fixed volume of inoculum (OD = 0.05) incubated at 30˚C for 7 days. The LB plates with 1% NaCl (w/v) were used as control [13].…”

Section: Isolation Of Rhizobacteria and Halo-tolerance Assaymentioning

confidence: 99%

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

et al. 2020

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“…The bacterial colonies were isolated and the pure cultures were separated based on their morphological characteristics. The isolates were identified by conducting the microscopic examination followed by biochemical tests and comparing the results obtained in Bergey's manual (Faridi et al 2015) and Advanced Bacterial Identification Software (ABIS) (Rahman et al 2017). The fungal isolates were identified using both microscopic and macroscopic examinations following the identification manual (Barnett and Hunter 2010).…”

Section: Microbial Analysismentioning

confidence: 99%

Ambient bioaerosol distribution and associated health risks at a high traffic density junction at Dehradun city, India

Madhwal

Prabhu

Sundriyal

et al. 2020

Environ Monit Assess

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Traffic junctions are one of the crowded places where commuters are at high risk of developing respiratory infections, due to their greater exposure to airborne and human transmitted microbial pathogens. An airborne bioaerosol assessment study was carried out at a high traffic density junction focusing on their concentration, contribution in respirable particulate matter (PM), and factors influencing the distribution and microbial diversity. Andersen six-stage viable cascade impactor and a wide-range aerosol spectrometer were used for microbial and particulate matter measurements, respectively. Statistical analysis was conducted to evaluate the relationship between bioaerosol concentration, vehicular count, PM concentration, and meteorological parameters. The mean bacteria concentration (1962.95 ± 651.85 CFU/m 3 ) was significantly different than fungi (1118.95 ± 428.34 CFU/m 3 ) (p < 0.05). The temporal distribution showed maximum concentration for bacteria and fungi during monsoon and postmonsoon seasons, respectively. In terms of bioaerosol loading, a considerable fraction of fungi (3.25%) and bacteria (5.65%) contributed to the total airborne PM. Most abundant bioaerosols were Aspergillus (27.58%), Penicillium (23%), and Cladosporium (14.05%) (fungi), and Micrococcus (25.73%), Staphylococcus (17.98%), and Bacillus (13.8%) (bacteria). Trafficinduced roadside soil resuspension and microbial aerosolizations from the human body were identified as the chief sources of bioaerosol emissions. The risk of lower respiratory tract infections caused by anthroponotic (human transmitted) transfer of bacterial pathogens is very high. The results of the study can be used to trace sources of microbial mediated communicable diseases, and to recommend appropriate safety measures to avoid pathogenic bioaerosol exposure.

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“…Halotolerant bacteria, recovered from the composting process, were able to produce hydrolases, lipases, proteases, amylases, cellulases and biopolymers. Slightly tolerant as pseudomonads, enterobacteria, and vibrios, can survive in up to 2-8%, moderately tolerant 18-20% and extremely tolerant microbes can grow over the whole range of salt concentrations from zero to saturation 26 . From these reason most of Bacillus that we obtained were halotolerant.…”

Section: Discussionmentioning

confidence: 99%

Halotolerant Bacillus sp. for Mannan Degradation Isolated from Mangrove Ecosystem at Hanura Beach Lampung

Sumardi¹,

Farisi²,

Ekowati³

et al. 2020

J Pure Appl Microbiol

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Mannose and mannooligosaccharide acted as prebiotic that consumed by probiotic bacteria. Mannanase were the second most important enzymes for the hydrolysis of hemicelluloses, beside xylanase. The objective research was to obtain eminent strains of Bacillus sp. in mannan degradation which can potentially be a probiotic candidate. The study employed a completely randomized design using four concentration levels repeated six times. Halotolerant Bacteria were isolated from mangrove ecosystem at Hanura beach, Teluk Pandan, Pesawaran District in Lampung Province. They are grown on the sea water complete agar media. Mannan degradation isolates were then characterized to determine their character with a variety of tests, including resistance to pH, salt, and metal ions, pathogenicity, and determination of mannanase production duration. Thirty strains are found to grow at 3-6% salt content, and 9 of them have mannanolytic activity. They grow optimally at pH 7-10. Seven isolates were proven to be positively hydrolyzed blood agar in the pathogenicity test. The addition of Iron (III) Chloride increased the enzyme activity by 11.12% in IBK3 isolates at 96 hours of cultivation period which was 0.05 UmL-1. It acted as cofactors of enzymatic reactions. Strains Bacillus sp., were able to degrade mannan substrate. It quantified using Index of mannanolytic. Strain IBK3 has the highest index of mannanolytic activity as much as 10.74. Their ability to grow in salt media indicated that they were halotolerant. They were more likely to live at base rather than acid habitat. Only IBK3 and ID2K1 showed non-pathogenic isolates. Only FeCl 3 addition has proven to rise up enzymatic activity.

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Isolation and identification of halotolerant soil bacteria from coastal Patenga area

Cited by 32 publications

References 20 publications

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

Ambient bioaerosol distribution and associated health risks at a high traffic density junction at Dehradun city, India

Halotolerant Bacillus sp. for Mannan Degradation Isolated from Mangrove Ecosystem at Hanura Beach Lampung

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