Isolation and Identification of Endophytic Fungi from Leave and Stem of Calopogonium mucunoides

Fitriarni, Dian; Kasiamdari, Rina Sri

doi:10.22146/jtbb.32477

Cited by 18 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…was from the stems. There are factors determined the presence of endophytic fungi in plants, such as environment, species, and isolation technique [11]. Figures 1 and 2 display the macroscopic and microscopic morphologies of identified endophytic fungi that were observed and recorded based on the Illustrated Genera of Imperfect Fungi Handbook [10].…”

Section: Resultsmentioning

confidence: 99%

Isolation and identification of endophytic fungi from mandarin orange (Citrus reticulata L.)

Oktarina

Adithia

Chamzurni

2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Endophytic fungi are recognised for producing secondary metabolites that have an effect on the host, such as promoting growth and enhancing plant resilience to biotic and abiotic stress. The objective of this study was to explore endophytic fungi from Citrus reticulata. The endophytic fungi were isolated from both healthy and infected roots, stems, and twigs of C. reticulata. The materials were sterilised and inoculated on potato dextrose agar (PDA) media. The fungi grown were transferred onto fresh PDA plates and identified based on their morphological characteristic, including colonial features, hyphae type, and reproduction structure. Five endophytic fungi were identified as Colletotrichum sp. AJSH2-1, Cylindrocladium sp. BJSH1-2, Rhizopus sp. RJSH1-1, Mucor sp. RJSH1-2, and Aspergillus sp. BJSH1-1. Further study is required to understand the ability of the identified genera in protecting the host from plant disease as well as promoting growth.

show abstract

Section: Resultsmentioning

confidence: 99%

Isolation and identification of endophytic fungi from mandarin orange (Citrus reticulata L.)

Oktarina

Adithia

Chamzurni

2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…Endophytic fungus isolated from the leaves, stem bark, and root bark of S. malaccense plants from Palembang, South Sumatra, produced various antibacterial substances depending on their taxonomic groupings. These three plant parts could have varied functions in supporting host growth and nutritional needs in pathogen protection (Selim et al 2012;Fitriarni and Kasiamdari 2018). Endophytic fungi create secondary compounds that protect host plants against pathogens, consequently, plant parts can produce a variety of endophytic fungal isolates (Fadiji and Babalola 2020).…”

Section: Discussionmentioning

confidence: 99%

Biodiversity and antibacterial activity of endophytic fungi isolated from jambu bol (Syzygium malaccense)

et al. 2021

View full text Add to dashboard Cite

Abstract. Hapida Y, Elfita, Widjajanti H, Salni. 2021. Biodiversity and antibacterial activity of endophytic fungi isolated from jambu bol (Syzygium malaccense). Biodiversitas 22: 5668-5677. Infectious diseases caused by pathogenic bacteria are still high. The existence of bacterial resistance to antibiotics makes it necessary to search for new antibacterial substances from plants and microorganisms. Syzygium malaccense (L.) Merr and Perry (Myrtaceae), known as jambu bol, has been used as a traditional medicine in Indonesia and other countries. It is used to treat pathogenic bacterial infections. Therefore, this study aims to determine the diversity of endophytic fungi and their potential as antibacterial compound sources compared to their host plants. Specifically, screening for endophytic fungi that have antibacterial activities is intended. In this study, endophytic fungi were isolated from the leaves, stem bark, and root bark of the host plants. Each isolate was identified morphologically and cultivated in potato dextrose broth (PDB) medium for four weeks. After the incubation period, the liquid culture was extracted with ethyl acetate and evaporated to obtain a concentrated extract. The Kirby-Bauer method was used to evaluate the antibacterial activity of each endophytic fungal extract and the host plant parts extract. The concentration used was 400µg/mL and the antibiotic was used as a positive control (Tetracycline) is 30µg/mL. Seven endophytic fungi were isolated from the leaves (YD1, YD2, YD3, YD4, YD6, YD7, Y2D1), seven from the stem bark (YB1, YB2, YB3, YB4, YB5, Y2B5, and Y2B6 ), and six from the root bark (YA1, YA2, YA3, YA4, YA5, Y2A2). The endophytic fungi were morphologically identified, and the isolates were shown to be dominated by genera from the Ascomycota (e.g., Poaceascoma, Cladorrhinum, Penicillium, Madurella, Phytophthora, Phialemonium, Monascus, Trichoderma, Ramophialophora, Gliocladium, Wiesneriomyces) and the Zygomycota (e.g., Mucor, Mortierella, and Gongronella) phyla. The fungi isolates Y2D1, YB1, and YA4 were shown to have the highest (strong) antibacterial activity equivalent to their hosts in the performed screening. Endophytic fungi have significant and continued antibacterial activities that have been identified molecularly.

show abstract

“…Purification was carried out by transferring the colonies to a new PDA medium with a single spore isolation method, then incubated at room temperature for 2 x 24 hours. The purified fungal colonies were re-cultured on PDA media as working culture (in Petri dishes) and stock cultures (in test tubes) (Fitriarni and Kasiamdari 2018;Hanin and Fitriasari 2019;Habisukan et al 2021).…”

Section: Isolation Of Endophytic Fungimentioning

confidence: 99%

Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

et al. 2021

View full text Add to dashboard Cite

Abstract. Syarifah, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite. Biodiversitas 22: 4572-4582. The decoction of the root bark of Syzygium zeylanicum has been used as traditional medicine, such as for treating pathogenic bacterial infections. Endophytic fungi that live in medicinal plant tissues have a high species diversity and biological activities correlate with their host. Therefore, this study aimed to explore the diversity of endophytic fungi from the root bark of S. zeylanicum and to determine the antibacterial activity of endophytic fungi and their secondary metabolites. In this study, we isolate and identify the endophytic fungi from the root bark of S. zeylanicum, continued by screening their antibacterial activity against two Gram-negative bacteria (Escherichia coli InaCCB5 and Salmonella thypi ATCC1048 and two Gram-positive bacteria (Staphylococcus aureus InaCCB4 and Bacillus subtilis InaCCB1204) by the Kirby-Bauer method. The fungal extract with the highest antibacterial activity proceeded with the isolation and determination of the structure of their bioactive compounds. The isolates were morphologically identified. Isolates that showed strong antibacterial activity were identified by molecular identification. Isolation of bioactive compounds was carried out by chromatographic techniques and the determination of the structure of pure chemical compounds was performed by the spectroscopic analysis. In total, there were 8 isolates of endophytic fungi were obtained from the root bark of S. zeylanicum, namely SZR1 – SZR8. SZR2 isolate has the highest antibacterial activity. Molecular identification through phylogenetic analysis showed that SZR2 isolate had high similarity with Penicillium brefeldianum. Isolation of bioactive compounds from SZR2 produced compound 1 in the form of light yellow crystals which showed strong antibacterial activity against S. typhi, E. coli, and B. subtilis with MIC values of 64 g/mL. Compound 1 was identified as p-hydroxybenzaldehyde, which was also obtained in its host. In conclusion, the endophytic fungus Penicillium brefeldianum produces similar secondary metabolites and antibacterial activity as its host plant.

show abstract

Isolation and Identification of Endophytic Fungi from Leave and Stem of Calopogonium mucunoides

Cited by 18 publications

References 0 publications

Isolation and identification of endophytic fungi from mandarin orange (Citrus reticulata L.)

Isolation and identification of endophytic fungi from mandarin orange (Citrus reticulata L.)

Biodiversity and antibacterial activity of endophytic fungi isolated from jambu bol (Syzygium malaccense)

Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

Contact Info

Product

Resources

About