Isolation and identification of bacteria from fresh guava (Psidium guajava) sold at local markets in Mymensingh and their antibiogram profile

Sarker, Atiqur Rahman; Haque, Md. Fazlul; Rifa, Rafia Afroze; Ema, Fateha Akther; Islam, Ariful; Khatun, Mst. Minara

doi:10.14202/vetworld.2018.1145-1149

Cited by 10 publications

(12 citation statements)

References 10 publications

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“…Apart from orange juice collected at TN and RO and sugarcane juice sold by the vendors of TN, the presence of Vibrio contamination in our study areas were negligible. Previous studies also found the similar low Vibrio contamination of fruit juices when compared with E. coli and Salmonella contamination [24,42,43].…”

Section: Discussionsupporting

confidence: 65%

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Antibiotic Resistance in Microbes from Street Fruit Drinks and Hygiene Behavior of the Vendors in Delhi, India

Sharma

Singh

Toor

et al. 2020

IJERPH

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Microbial contamination of fruit juices has caused major outbreaks, leading to significant morbidity and mortality in developing countries. The inept hygiene and safety practices followed by the juice vendors are the leading risk factors of the microbial contamination of juices. In this pilot study, the five most crowded markets in urban Delhi, including Kamla Nagar, University of Delhi (north campus), Tilak Nagar, Chandni Chowk, and Rohini, were selected for a questionnaire survey on the fruit juice vendors and the sampling of water used for juice preparation as well as sugarcane, orange, and mix fruit juices collected from these markets for the enumeration of total bacterial count (TBC), Escherichia coli, Salmonella, and Vibrio. Antibiotic susceptibility tests were performed using ampicillin, cefotaxime, chloramphenicol, ciprofloxacin, and imipenem. The results indicated that the majority of the vendors were not following hygiene and safety practices when compared with the recommended standard safety practices. The use of municipal water by 95% of vendors with high TBC counts might have been the major source of microbial contamination in all types of fruit juices. E. coli and Salmonella contaminations were high in sugarcane (2 × 105 colony forming units (CFU)/mL) and mix fruit (2.2 × 105 CFU/mL) juice samples, respectively. On the other hand, Vibrio was found to be absent in almost all juice samples except for orange juice. All strains were found to be susceptible to chloramphenicol, but resistant to ampicillin and cefotaxime. Only a few strains were resistant to ciprofloxacin, and only E. coli strains were resistant to imipenem. Taken together, the overall microbiological standards of fruit juices served by street vendors were not within the acceptable limits, perhaps due to the poor quality of water used to prepare juices and poor hygiene and safety practices followed by the vendors. More importantly, the isolated microbes demonstrated resistance to ampicillin and cefotaxime, which may have pressing public health implications. Post hoc power analyses identified the minimum sample size required for 80% power.

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Section: Discussionsupporting

confidence: 65%

“…Previous studies have reported the presence of AMR bacteria in apple and orange juice samples [21][22][23][24][25][26][27]. A recent study in India found 80% of the bacterial strains isolated from orange juice samples to be multi-drug resistant [28].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance in Microbes from Street Fruit Drinks and Hygiene Behavior of the Vendors in Delhi, India

Sharma

Singh

Toor

et al. 2020

IJERPH

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“…Moreover, similar findings were recorded in a study on fresh fruit juices in Dhaka City, Bangladesh [55]. In another report, in Mymensingh District, Bangladesh, a total of 25 fresh-cut guava samples were collected, and a TVBC range of 6.47-6.62 log CFU/mL was detected in the fruit samples [34]. Similarly, the average TVBC range was 6.47 log CFU/g in our study.…”

Section: Discussionsupporting

confidence: 91%

“…The present study evaluated the total microbial load on fresh fruits and vegetables (FFV) and concentrated on the safety of FFV by eliminating microorganisms. The indicator bacteria (TCC and TFC), the total viable bacteria (TVBC), and the total presence of fungi on the FFV surface reflect the sanitary nature of Agricultural products [34][35][36]. The isolated bacterial strains with higher TVBC were observed in studied fruits and vegetables (Tables 1 and 2), and similar findings were also obtained in other studies [46,47].…”

Section: Discussionsupporting

confidence: 85%

“…Besides this, water supplemented with varying concentrations of organic acids, likely acetic acid and citric and sorbic acids, has been shown to reduce microbial populations on fruit and vegetables [32,33]. However, there are very few reports of microbial contamination of fresh-cut vegetables and fruit in Bangladesh [34][35][36]. There is a very limited amount of microbial quality evaluation research and investigation of effectiveness of low-cost disinfectants of fresh fruit and vegetables has been carried out in Dhaka City to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 99%

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Microbial Quality Assessment and Efficacy of Low-Cost Disinfectants on Fresh Fruits and Vegetables Collected from Urban Areas of Dhaka, Bangladesh

Rahman

Azad

Uddain

et al. 2021

Foods

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This study aimed to examine the total viable bacteria (TVBC); total coliform (TCC); fecal coliform (TFC); pathogenic Pseudomonas spp., Staphylococcus aureus, and total fungi (TF); and the effect of different low-cost disinfectants (sterile water, salt water, blanched, and vinegar) in decontamination of 12 types of fruit and 10 types of vegetables. In fruit samples, the lowest TVBC was enumerated at 3.18 ± 0.27 log CFU/g in Indian gooseberry and the highest at 6.47 ± 0.68 log CFU/g in guava. Staphylococci (2.04 ± 0.53–5.10 ± 0.02 log CFU/g), Pseudomonas (1.88 ± 0.03–5.38 ± 0.08 log CFU/g), and total fungi (2.60 ± 0.18–7.50 ± 0.15 log CFU/g) were found in all fruit samples; however, no Salmonella was detected in fruit samples. Similarly, the lowest TVBC recorded 5.67± 0.49 log CFU/g in cucumber and the highest 7.37 ± 0.06 log CFU/g in yard long bean. The Staphylococci (3.48 ± 0.13–4.81 ± 0.16 log CFU/g), Pseudomonas (3.57± 0.21– 4.75 ± 0.23 log CFU/g), TCC (1.85 ± 1.11–56.50 ± 37.14 MPN/g), TFC (1.76 ± 0.87– 3.78 ± 3.76 MPN/g), and TF (3.79 ± 0.18–4.40 ± 0.38 log CFU/g) were recorded in all vegetables samples, but no Salmonella was detected in yard long bean, pointed gourd, carrot, tomato, cucumber, or brinjal. However, vinegar showed the highest microbial load reduction of selected fruit and vegetables among the different treatments. With vinegar treatment, the highest reduction of TVBC (1.61-log) and TF (2.54-log) was observed for fruits, and TVBC (2.31-log) and TF (2.41-log) for vegetables. All the disinfectant treatments resulted in significant (p < 0.01) bacterial load reduction compared to control for the studied fruits and vegetable samples.

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The Inhibition Effect and Mechanism of Nano Magnesium Peroxide Against Spoilage Fungi Emerging in Hami Melon

Xiao

Ying-ji

et al. 2023

Food Bioprocess Technol

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Isolation and identification of bacteria from fresh guava (Psidium guajava) sold at local markets in Mymensingh and their antibiogram profile

Cited by 10 publications

References 10 publications

Antibiotic Resistance in Microbes from Street Fruit Drinks and Hygiene Behavior of the Vendors in Delhi, India

Antibiotic Resistance in Microbes from Street Fruit Drinks and Hygiene Behavior of the Vendors in Delhi, India

Microbial Quality Assessment and Efficacy of Low-Cost Disinfectants on Fresh Fruits and Vegetables Collected from Urban Areas of Dhaka, Bangladesh

The Inhibition Effect and Mechanism of Nano Magnesium Peroxide Against Spoilage Fungi Emerging in Hami Melon

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