Holigarna caustica (Dennst.), a popular plant used in folk medicine in Bangladesh, is often used by the local folk practitioner to treat a variety of chronic diseases. The present research is an attempt to find out an innovative therapeutic prospect for the management of neuropsychiatric disorders. The methanol extract of H. caustica leaves (MEHC) were utilized on various behavioral tests for assessing anxiolytic, anti-depressant, and anti-inflammatory activities. The antioxidant potentials and quantitative phytochemicals were evaluated through spectrophotometric methods. Results revealed that treatment of MEHC (200 and 400 mg/kg) significantly reduced anxiety like behaviors in mice, particularly, 400 mg/kg efficiently improved % of entries and time spent (p < 0.05) in the open arms in elevated plus maze test, whereas, superior head dipping tendency (p < 0.05) was observed in hole-board test. In contrast, mice treated with 200 mg/kg revealed better anxiolytic effect in both open field and hole-cross tests. During antidepressant evaluation, mice administrated with MEHC exhibited active behaviors (swimming and struggling) in forced swimming and tail suspension tests. In parallel, MEHC manifested a noteworthy (p < 0.001) suppression of inflammatory response induced by histamine. The MEHC also showed strong antioxidant activities in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) (IC50: 57.64 μg/mL) scavenging, H2O2 (IC50: 51.60 μg/mL) scavenging, and ferric reducing power assay. The levels of total phenol, flavonoid, flavonol, condensed tannin, and antioxidant were estimated as higher in MEHC. Moreover, 11 compounds were documented as bioactive, displayed good binding affinities to potassium channel receptor, human serotonin receptor, cyclooxygenase (COX-1 and 2), and xanthine oxidoreductase enzyme targets in molecular docking experiments. Furthermore, ADME/T and Prediction of Activity Spectra for Substances (PASS) analyses exposed their drug-likeness, nontoxic upon consumption, and likely pharmacological actions. Overall, the H. caustica is potentially bioactive as evident by in vivo, in vitro, and computational analysis. Our findings support the folkloric value of this plant, which may provide a potential source towards developing drug leads.

This study was carried out to develop a high-performance liquid chromatography method for short-time analysis of the main cannabinoids in the inflorescence of hemp (Cannabis sativa L.). We also performed decarboxylation of the raw material using our advanced analysis technique. In this study, the UV spectrum was considered to analyze each of the four common cannabinoids, solvents, and samples, where the uniform elution of acidic cannabinoids without peak tailing and acids was tested. Optimal results were obtained when readings were taken at a wavelength of 220 nm using water and methanol containing trifluoroacetic acid as mobile phases in a solvent gradient system. The established conditions were further validated by system suitability, linearity, precision, detection limit, and quantitation limit tests. The decarboxylation index (DT50) confirmed that Δ9-THCA decarboxylated faster than CBDA, and both maintained a linear relationship with time and temperature. In addition, the loss of cannabidiol was better prevented during the decarboxylation process in the natural state than in the extracted state.

This study aimed to examine the total viable bacteria (TVBC); total coliform (TCC); fecal coliform (TFC); pathogenic Pseudomonas spp., Staphylococcus aureus, and total fungi (TF); and the effect of different low-cost disinfectants (sterile water, salt water, blanched, and vinegar) in decontamination of 12 types of fruit and 10 types of vegetables. In fruit samples, the lowest TVBC was enumerated at 3.18 ± 0.27 log CFU/g in Indian gooseberry and the highest at 6.47 ± 0.68 log CFU/g in guava. Staphylococci (2.04 ± 0.53–5.10 ± 0.02 log CFU/g), Pseudomonas (1.88 ± 0.03–5.38 ± 0.08 log CFU/g), and total fungi (2.60 ± 0.18–7.50 ± 0.15 log CFU/g) were found in all fruit samples; however, no Salmonella was detected in fruit samples. Similarly, the lowest TVBC recorded 5.67± 0.49 log CFU/g in cucumber and the highest 7.37 ± 0.06 log CFU/g in yard long bean. The Staphylococci (3.48 ± 0.13–4.81 ± 0.16 log CFU/g), Pseudomonas (3.57± 0.21– 4.75 ± 0.23 log CFU/g), TCC (1.85 ± 1.11–56.50 ± 37.14 MPN/g), TFC (1.76 ± 0.87– 3.78 ± 3.76 MPN/g), and TF (3.79 ± 0.18–4.40 ± 0.38 log CFU/g) were recorded in all vegetables samples, but no Salmonella was detected in yard long bean, pointed gourd, carrot, tomato, cucumber, or brinjal. However, vinegar showed the highest microbial load reduction of selected fruit and vegetables among the different treatments. With vinegar treatment, the highest reduction of TVBC (1.61-log) and TF (2.54-log) was observed for fruits, and TVBC (2.31-log) and TF (2.41-log) for vegetables. All the disinfectant treatments resulted in significant (p < 0.01) bacterial load reduction compared to control for the studied fruits and vegetable samples.

The performance of hemp seedlings was evaluated through morphological traits, photosynthetic pigments, and osmolytes under 11 light treatments (10 LED light compositions + natural light) in an aeroponics system. The seedlings were brought under treatment at 25 days of age, where the light intensity was 300 µmol m−2s−1 and duration was 20 days. A higher leaf number and node number were observed in L10 (R4:B2:W2:FR1:UV1) and L11 (R2:B2:G2:W2:FR1:UV1), and a higher leaf length and leaf width were recorded in the L2 (white), L3 (R8:B2), and L5 (R7:B2:FR1) treatments. Furthermore, a higher shoot length was recorded in L3 (R8:B2), L6 (R6:B2:G1:FR1), and L9 (R6:B2:FR1:UV1) while roots developed more in the L1 (natural light), L5 (R7:B2:FR1), and L9 (R6:B2:FR1:UV1) treatments. On the other hand, the L3 (R8:B2) treatment manifested higher chlorophyll a, chlorophyll b, and photosynthetic quantum yield (Fv/Fm). The hierarchical clustering and heatmap analysis revealed that higher leaf numbers and node numbers resulted in bushy plants with shorter shoots and longer roots. A negative correlation was also observed in photosynthetic traits (pigments and fluorescence) with osmolytes and root length. Importantly, the treatments L4 (R7:B2:G1), L6 (R6:B2:G1:FR1), L8 (R5:B2:G1:FR1:UV1), and L11 (R2:B2:G2:W2:FR1:UV1) manifested higher nodes with a higher osmolyte content, such as proline, ascorbic acid, total soluble carbohydrate, and sucrose, which may be a helpful indicator for higher branches and inflorescences, and ultimately higher cannabinoids accumulation in the plants. The approach and findings of this study could provide future research with the baseline information on optimizing the light composition to produce hemp plants with ideal phenotypes.

Production of plants under artificial light conditions is an innovative and smart concept to grow food year-round in any location. However, pre-basic seed potato production in the greenhouse from LED pre-treated seedlings under an aeroponic system is a new and creative idea. Therefore, the objective of the study was to optimize the effect of LED pre-treatment and determine the best LED spectral composition on growth performance and tuberization of potato plants. Potato variety ‘Golden King’ was treated under 9 LED light spectra for 30 days—L1 (natural light), L2, (R:B), L3 (R:B:G), L4 (R:B:FR), L5 (R:B:G:FR), L6 (R:B:G:FR:UV), L7 (R:B:FR:UV), L8 (R:B:W:FR), and L9 (R:B:W:FR:UV) under 300 µmol m−2 s−1 photosynthetic photon flux density (PPFD), 23/15 °C (day/night) temperature, and 70% relative humidity. The study revealed that growth characteristics and tuber number for plants were increased most by the light spectrum L4 (R:B:FR). Furthermore, photosynthetic pigments increased in L4, L7, and L8, while TSC and sucrose accumulated more in L1 treatment. In contrast, higher seed tuber fresh weight was recorded in L8, L9, L4, and L7. Overall, it can be concluded that potato seedlings pre-treated with the L4 (R:B:FR) LED spectral composition performed best for growth, establishment, and tuberization.