Isolation and identification of a variant subtype G 2b porcine epidemic diarrhea virus and S gene sequence characteristic

Zhu, Tongjia; Du, Shuaishuai; Cao, Dongmei; Pei, Zhihua; Guo, Yi; Shao, Hongxia; Wang, Haijun; Wang, Kai; Hu, Guang‐Wan

doi:10.1016/j.meegid.2019.03.015

Cited by 14 publications

(16 citation statements)

References 33 publications

(36 reference statements)

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“…Moreover, the viral loads were notably high in the duodenum, jejunum, ileum, cecum, and colon of the animals infected with strain HM2017, which is agreement with the report by Jung et al (2014) [32]. Additionally, PEDV strain CH/JLDH/2016, which was classified into subgroup GII-a, was also highly pathogenic to piglets [18]. These data demonstrate that the PEDV strains of the developing GII-a subgroup are highly virulent and pose a serious threat to the pig populations in China.…”

Section: Discussionsupporting

confidence: 91%

“…The PEDV subgroups GII-a and GIIb in China are highly pathogenic to sucking piglets and exhibit serum cross-neutralization activity or cross-immune protection. The GII-a subgroup of PEDV was found to mediate better immune protection against challenges with the homologous PEDV strain than with the GIIb subgroup strain [5,18,22,23]. The objective of the current study was to reveal the molecular characteristics of the isolated subgroup GII-a PEDV strain HM2017, and evaluate its molecular characteristics and pathogenesis.…”

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confidence: 98%

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Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China

Yang

et al. 2020

Microbial Pathogenesis

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Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 98%

Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China

Yang

et al. 2020

Microbial Pathogenesis

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“…In addition, PEDVs can be divided into genome 1 (G1) and genome 2 (G2) clades and further divided into four subgroups: G1a, G1b, G2a, and G2b (Wang et al, 2016). Despite they have relatively conserved neutralizing epitopes, commercial vaccines based on CV777 strains cannot provide complete protection in piglets against variant PEDV infections which are currently circulating in China and the US (Zhu et al, 2019). Monitoring the genetic alterations in the S gene of PEDV could provide more clues for understanding the epidemic characteristics of PEDV and adjusting the immunization program of PED in pig farms.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of a Recombinant Porcine Epidemic Diarrhea Virus With a Novel Insertion in S1 Domain

Liu

et al. 2021

Front. Microbiol.

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Porcine epidemic diarrhea virus (PEDV) is the major pathogen that causes diarrhea and high mortality in newborn piglets with devastating impact to the pig industry. Recombination and mutation are the main driving forces of viral evolution and genetic diversity of PEDV. In 2016, an outbreak of diarrhea in piglets occurred in an intensive pig farm in Central China. A novel PEDV isolate (called HNAY) was successfully isolated from clinical samples. Sequence analysis and alignment showed that HNAY possessed 21-nucleotide (nt) insertion in its S1 gene, which has never been reported in other PEDV isolates. Moreover, the sequence of the insertion was identical with the sequence fragment in PEDV N gene. Notably, the HNAY strain exhibited two unique mutations (T500A and L521Y) in the neutralizing epitopes of the S1 protein that were different from those of other PEDV variant strains and CV777-based vaccine strains. Additionally, PEDV HNAY might be derived from a natural recombination between two Chinese variant PEDV strains. Animal experiments demonstrated that HNAY displayed higher pathogenicity compared with two other clinical isolates. This study lays the foundation for better understanding of the genetic evolution and molecular pathogenesis of PEDV.

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“…In the 1980s, PEDV was rst identi ed in China, and the virus has caused huge economic losses [9]. Although a PEDV vaccine has been used to immunize pigs during intensive farming, piglet epidemic diarrhea still occurs occasionally [10]. Maternal immunity can provide protection for piglets against PEDV infection [5].…”

Section: Introductionmentioning

confidence: 99%

“…Milk sEVs mediate absorptive epithelium renewal, cell viability, and proliferation in the neonatal intestine after birth [19]. Our laboratory has established a method for the isolation and identi cation of porcine milk-derived sEVs, and we have identi ed their miRNAs as novel regulatory molecules transmitted mother-to-offspring [10]. Our previous studies have shown that porcine milk sEVs facilitated intestinal tract development [20] and prevented LPS-induced intestinal damage [21].…”

Section: Introductionmentioning

confidence: 99%

Milk Small Extracellular Vesicles-derived miRNAs Restrict Porcine Epidemic Diarrhea Virus Infectivity

Xie¹,

Hou

Song

et al. 2021

Preprint

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BackgroundVirus-caused diseases are a huge challenge to both animals and human beings, especially coronaviruses. Porcine epidemic diarrhea virus (PEDV), a coronavirus, causes acute diarrhea and up to 100% mortality in piglets less than three weeks of age. Maternal immunity provides protection for piglets in resisting PEDV infection. Small extracellular vesicles (sEV) contain bioactive molecules such as miRNAs to exchange genetic and epigenetic information between cells. Our previous study suggested that milk sEV facilitated intestinal tract development and prevented LPS-induced intestine damage. However, the effects of milk sEV on the inhibition of viral infections remain unclear. ResultsIn this study, through in vivo experiments, we found that porcine milk sEV protected piglets from PEDV-induced diarrhea and death. In vitro, we clarified that this protective effect was partly generated through the inhibition of the PEDV-N protein and HMGB1 by sEV miR-let-7e and miR-27b, respectively. ConclusionsIn conclusion, we report that porcine milk sEVs protected piglets from PEDV-induced diarrhea and death by inhibiting virus replication, and this protective effect was partly generated through the inhibition of the PEDV-N and HMGB1 pathways by exosomal miR-let-7e and miR-27b. This study reveals a new antiviral function of milk sEVs, and the results suggest that milk sEVs may act as a mother-offspring transmission pathway for protecting newborns against PEDV infection.

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Isolation and identification of a variant subtype G 2b porcine epidemic diarrhea virus and S gene sequence characteristic

Cited by 14 publications

References 33 publications

Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China

Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China

Isolation and Identification of a Recombinant Porcine Epidemic Diarrhea Virus With a Novel Insertion in S1 Domain

Milk Small Extracellular Vesicles-derived miRNAs Restrict Porcine Epidemic Diarrhea Virus Infectivity

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