Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China

Yang, Dan; Su, Mingjun; Li, Chunqiu; Zhang, Bei; Qi, Shanshan; Sun, Dongbo; Yin, Baoshu

doi:10.1016/j.micpath.2019.103922

Cited by 25 publications

(27 citation statements)

References 39 publications

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“…Point mutations, insertions, or deletions in structural proteins and non-structural proteins have resulted in changes in tropism and virulence of coronaviruses [17][18][19]. In the case of PEDV, deletions or insertions are frequently observed in the spike protein or ORF3 [20][21][22]. For example, HM2017 has two insertions in the S gene [20], HLJBY has a 133-aa deletion in ORF3 [21], and 15 novel eld PEDV variants with large genomic deletions have been identi ed in Japan [22].…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of a Variant Subgroup GII-a Porcine Epidemic Diarrhea Virus Strain in China

Tian¹,

Lian²,

Hu³

et al. 2020

Preprint

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Background: Since 2010, variant porcine epidemic diarrhea virus (PEDV) has emerged in several provinces of China. The variant strain causes high mortality of newborn piglets, which has resulted in severe economic losses to the pork breeding industry.Results: A variant PEDV strain, SC-YB73, was isolated and identified in China. Pathological observation showed atrophy of villi and edema in the lamina propria. Sequence analysis indicated that six nucleotides were inserted in the E gene, which was first detected in PEDV strains. Furthermore, fifty nucleotide sites were unique in SC-YB73, when compared with another 28 PEDV strains. Phylogenetic analysis based on the complete genome showed that SC-YB73 was clustered in the variant subgroup GII-a, which is widely prevalent in the pig population in China. Recombination analysis suggested that SC-YB73 originated from the recombination of GDS47, TW/Yunlin550/2018, and COL/Cundinamarca/2014.Conclusion: The present study involved the isolation and genetic characterization of a variant PEDV strain. It will provide essential information for the control of PED outbreaks in China.

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Section: Discussionmentioning

confidence: 99%

“…In the case of PEDV, deletions or insertions are frequently observed in the spike protein or ORF3 [20][21][22]. For example, HM2017 has two insertions in the S gene [20], HLJBY has a 133-aa deletion in ORF3 [21], and 15 novel eld PEDV variants with large genomic deletions have been identi ed in Japan [22].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of a Variant Subgroup GII-a Porcine Epidemic Diarrhea Virus Strain in China

Tian¹,

Lian²,

Hu³

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…The outbreak of PEDV was partly due to the rapid onset of the virus, the rapid spread of the virus, the high morbidity and mortality of piglets, and the widespread distribution [ 8 , 9 ]. In subsequent outbreaks of swine diarrhea disease, PED showed a high positive rate, which had a strong negative impact on the swine industry [ 10 , 11 , 12 ]. In December 2010, a new highly virulent strain of PEDV broke out in China that killed more than 1 million piglets, and the mortality rate for suckling piglets amounted to 100% [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Application of a Reverse-Transcription Recombinase-Aided Amplification Assay for Porcine Epidemic Diarrhea Virus

Liu

Gao

et al. 2022

Viruses

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Porcine epidemic diarrhea virus (PEDV) is a coronavirus currently widespread worldwide in the swine industry. Since PEDV was discovered in China in 1984, it has caused huge economic losses in the swine industry. PEDV can infect pigs of all ages, but piglets have the highest infection with a death rate as high as 100%, and the clinical symptoms are watery diarrhea, vomiting, and dehydration. At present, there is not any report on PEDV detection by RT-RAA. In this study, we developed an isothermal amplification technology by using reverse-transcription recombinase-aided amplification assay (RT-RAA) combined with portable instruments to achieve a molecular diagnosis of PEDV in clinical samples from China. By designing a pair of RT-RAA primers and probes based on the PEDV N gene, this method breaks the limitations of existing detection methods. The assay time was within 30 min at 41 °C and can detect as few as 10 copies of PEDV DNA molecules per reaction. Sixty-two clinical tissue samples were detected by RT-qPCR and RT-RAA. The positive and negative rates for the two methods were 24.19% and 75.81%, respectively. Specificity assay showed that the RT-RAA had specifically detected PEDV and was not reactive for porcine parvovirus (PPV), transmissible gastroenteritis virus (TGEV), porcine circovirus type 2 (PCV2), porcine pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), swine flu virus (SIV), or porcine Japanese encephalitis virus (JEV). The results suggested that RT-RAA had a strong specificity and high detection sensitivity when combined with a portable instrument to complete the detection under a constant temperature of 30 min, which are more suitable for preventing and controlling PEDV onsite in China.

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“…The PEDV subgroups G2a and G2b circulating in China are highly pathogenic to suckling piglets and exhibit serum cross-neutralization activity or cross-immune protection. Moreover, it was found that the G2a subgroup strains of PEDV, compared with the G2b subgroup strains, could resist attack by homologous PEDV strains by mediating better immune protection [ 10 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Characteristics and Pathogenicity of a Novel Porcine Epidemic Diarrhea Virus with a Naturally Occurring Truncated ORF3 Gene

Zhang

Chen

et al. 2022

Viruses

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Porcine epidemic diarrhea virus (PEDV) is the major pathogen that causes diarrhea and high mortality in newborn piglets, with devastating impact on the pig industry. To further understand the molecular epidemiology and genetic diversity of PEDV field strains, in this study the complete genomes of four PEDV variants (HN2021, CH-HNYY-2018, CH-SXWS-2018, and CH-HNKF-2016) obtained from immunized pig farms in central China between 2016 to 2021 were characterized and analyzed. Phylogenetic analysis of the genome and S gene showed that the four strains identified in the present study had evolved into the subgroup G2a, but were distant from the vaccine strain CV777. Additionally, it was noteworthy that a new PEDV strain (named HN2021) belonging to the G2a PEDV subgroup was successfully isolated in vitro and it was further confirmed by RT-PCR that this isolate had a large natural deletion at 207–373 nt of the ORF3 gene, which has never been reported before. Particularly, in terms of pathogenicity evaluation, colostrum deprivation piglets challenged with PEDV HN2021 showed severe diarrhea and high mortality, confirming that PEDV HN2021 was a virulent strain. Hence, PEDV strain HN2021 of subgroup G2a presents a promising vaccine candidate for the control of recurring porcine epidemic diarrhea (PED) in China. This study lays the foundation for better understanding of the genetic evolution and molecular pathogenesis of PEDV.

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Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China

Cited by 25 publications

References 39 publications

Molecular Characterization of a Variant Subgroup GII-a Porcine Epidemic Diarrhea Virus Strain in China

Molecular Characterization of a Variant Subgroup GII-a Porcine Epidemic Diarrhea Virus Strain in China

Development and Application of a Reverse-Transcription Recombinase-Aided Amplification Assay for Porcine Epidemic Diarrhea Virus

Genetic Characteristics and Pathogenicity of a Novel Porcine Epidemic Diarrhea Virus with a Naturally Occurring Truncated ORF3 Gene

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