Isolation and identification of a nitrile hydrolyzing bacterium and simultaneous utilization of aromatic and aliphatic nitriles

Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5

“…UFMG-Y28 (Rezende et al 2000 ). In our previous studies, we reported butyramide and butyric acid as the metabolites of butyronitrile degradation (Mukram et al 2015 ). In the present investigation, we have carried out metabolite feeding experiment by allowing the organism to grow on the metabolites and then estimating the residual metabolite concentration in the culture medium.…”

“…Rhodococcus sp. MTB5 used in the present study was previously isolated in our laboratory (Mukram et al 2015 ). For butyronitrile degradation studies, the bacterium was grown in MS medium supplemented with butyronitrile as carbon and nitrogen source.…”

“…For butyronitrile degradation studies, the bacterium was grown in MS medium supplemented with butyronitrile as carbon and nitrogen source. Medium composition was the same as explained previously (Mukram et al 2015 ). The pH of the medium was adjusted to 7.0 using 2 N NaOH or 2 N HCl.…”

“…The butyramide and butyric acid are the two metabolic intermediates of butyronitrile degradation (Mukram et al 2015 ). For further confirmation of complete degradation of butyronitrile, we have checked the ability of the organism to utilize these intermediate metabolites as sole source of carbon and/or nitrogen.…”

“…Butyronitrile, butyramide and butyric acid concentrations were determined by gas chromatography (GC). The GC model used and the chromatographic conditions followed were as described previously (Mukram et al 2015 ).…”