The Use of Enzymes in Bioremediation of Soil Xenobiotics

Piotrowska-Długosz, Anna

doi:10.1007/978-3-319-47744-2_17

Cited by 8 publications

(2 citation statements)

References 86 publications

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“…As several studies reported [52][53][54] , applying specific enzymes in bioremediation offers some advantages compared to the use of microbial cells, including enhanced specificity, simplified handling and storage, higher www.nature.com/scientificreports/ mobility, and activity even in the presence of high concentrations of toxic contaminants. Furthermore, the enzymes do not require nutrient supplementation and may be applicable under extreme environmental conditions.…”

Section: Performance Of the Novel Enzymes In Pah Degradationmentioning

confidence: 99%

“…Furthermore, the enzymes do not require nutrient supplementation and may be applicable under extreme environmental conditions. Although the enzyme-based bioremediation methods seem advantageous clean-up technologies, some limitations of these technologies have been demonstrated [54][55][56] . Free enzymes are generally unstable, may be degraded and consequently exist for only a short time in a soil environment; the individually applied free enzymes cannot completely degrade the contaminants.…”

Section: Performance Of the Novel Enzymes In Pah Degradationmentioning

confidence: 99%

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Novel enzymes for biodegradation of polycyclic aromatic hydrocarbons identified by metagenomics and functional analysis in short-term soil microcosm experiments

Nagy,

Takács,

Németh

et al. 2024

Sci Rep

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Polycyclic aromatic hydrocarbons (PAHs) are highly toxic, carcinogenic substances. On soils contaminated with PAHs, crop cultivation, animal husbandry and even the survival of microflora in the soil are greatly perturbed, depending on the degree of contamination. Most microorganisms cannot tolerate PAH-contaminated soils, however, some microbial strains can adapt to these harsh conditions and survive on contaminated soils. Analysis of the metagenomes of contaminated environmental samples may lead to discovery of PAH-degrading enzymes suitable for green biotechnology methodologies ranging from biocatalysis to pollution control. In the present study, our goal was to apply a metagenomic data search to identify efficient novel enzymes in remediation of PAH-contaminated soils. The metagenomic hits were further analyzed using a set of bioinformatics tools to select protein sequences predicted to encode well-folded soluble enzymes. Three novel enzymes (two dioxygenases and one peroxidase) were cloned and used in soil remediation microcosms experiments. The experimental design of the present study aimed at evaluating the effectiveness of the novel enzymes on short-term PAH degradation in the soil microcosmos model. The novel enzymes were found to be efficient for degradation of naphthalene and phenanthrene. Adding the inorganic oxidant CaO2 further increased the degrading potential of the novel enzymes for anthracene and pyrene. We conclude that metagenome mining paired with bioinformatic predictions, structural modelling and functional assays constitutes a powerful approach towards novel enzymes for soil remediation.

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Section: Performance Of the Novel Enzymes In Pah Degradationmentioning

confidence: 99%

Section: Performance Of the Novel Enzymes In Pah Degradationmentioning

confidence: 99%