Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata

Abstract

The leaves of Mikania laevigata and Mikania glomerata are used in Brazil to treat respiratory affections, being kaurane-type diterpenes and coumarin considered as the bioactive compounds. The present study reports an investigation on the HPLC-DAD profi les and contents of coumarin (1), trans-o-coumaric (2), kaurenoic (3), benzoylgrandifl oric (4) and cinnamoylgrandifl oric (5) acids in dried leaves of Mikania species stored in dark room under controlled conditions. Excepting 2, the constituents were isolated and purifi ed to be employed as reference compounds. The samples were analyzed at three monthly intervals up to 18 months for M. laevigata and 12 months for M. glomerata. trans-o-Coumaric was not detected in both, whereas 1 occurred only in M. laevigata. The concentrations of the assayed constituents did not vary signifi cantly within the evaluated period (p vary signifi cantly within the evaluated period (p vary signifi cantly within the evaluated period ( < 0.05), for both species. In contrast, changes in the chromatographic profi les and spectral purity of peaks from 3, 4 and 5 were detected in samples of both Mikania stored for three months, while the coumarin profi le in M. laevigata modifi ed after six months of storage. The evaluation of chromatographic profi les based on spectral purity analyses of selected peaks was shown to be a more robust tool to access chemical stability of Mikania samples than the quantitation of chemical markers' contents.

COVID-19: Is There Evidence for the Use of Herbal Medicines as Adjuvant Symptomatic Therapy?

“…Chemical composition. Essential oil (germacrene D and β-caryophyllene as the major components) ( Ueno and Sawaya, 2019 ); coumarins (e.g., coumarin, trans- o -coumaric acid),; phenolic acids (e.g., chlorogenic and caffeoylquinic acids) ( Lazzari Almeida et al, 2017 ); terpenoids and steroids (e.g., friedelin, ent-kaurenoic acid, ent-kaur-16(17)-en-19-oic acid, ent-beyer-15(16)-en-19-oic acid, ent-15β-benzoyloxykaur16(17)-en-19-oic acid, grandifloric acid, ent-cinnamoylgrandifloric acid, ent-benzoylgrandifloric acid 17-hydroxy-ent-kaur-15(16)-en-19-oic acid, stigmasterol, β-sitosterol) ( Veneziani and Oliveira, 1999 ; Bertolucci et al, 2013 ).…”

Larvicidal potential of Mikania glomerata SPRENGEL extract on Ancylostoma caninum larvae

“…The presence of coumarin in M. glomerata samples was reported in several articles, but there are also articles reporting its absence, since this compound could not be quantified in the samples (Bertolucci et al, 2013). However, there are other metabolites described as a majority in the plant under study, like diterpenes, in particular the class of kauranes, present in the species M. glomerata have other pharmacological actions, particularly antiparasitic activity, and therefore further investigation is needed (Gasparetto et al, 2010).…”

Characterization and identification of filamentous bacterium XJ-16 and analysis of its bioactive components

“…The mobile phase was methanol: water in gradient elution, and the antibacterial ability of the eluate fractions was determined. Then, the fraction with antibacterial activity was prepared in large quantities under the same conditions, and the purity of the collected material was checked by HPLC on a Kromasil 100-5-C 18 column (4.6×250 mm) (Shanghai Mailing Technology Co., Ltd) (Bertolucci et al 2013). The injection volume was set to 20 µL, the ow rate was 1 mL/min, the detection wavelength was 280 nm, and the mobile phase was methanol: water (80:20).…”