Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia

Adal, Ayelign M.; Sarker, Lukman S.; Lemke, Ashley D.; Mahmoud, Soheil S.

doi:10.1007/s11103-017-0588-6

Cited by 19 publications

(13 citation statements)

References 61 publications

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“…The values we found in the composition of Lavandula angustifolia are in accord with previously data reported in the literature: 8.71% moisture, 9.16% ash, 6.82% protein, 6.89% lipids and 61.37% carbohydrates [11,12]. Experimental results on elemental composition reveal that calcium was the most abundant macro element, followed by potassium, magnesium and sodium.…”

Section: Chemical Compositionsupporting

confidence: 91%

Chemical Composition and the Potential of Lavandula angustifolia L. Oil as a Skin Depigmentant

Andrei¹,

Alexa²,

Tulcan³

et al. 2018

Rec.Nat.Prod.

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Finding non-invasive skin depigmenting agentsrepresent an important goal of cosmetic research and industry. It is now admited that Tyrosinase inhibitor substances could be the most efficient molecules in this field because Tyrosinase is the key enzyme in the melanogenesis process. In the pathology of macular skin hyperpigmentations it can mention aesthetic problems such as: melasma gravidarum, pigmentation of aged skin, photosensibilisation, hepatic disfunctions and post-inflammatory residual pigmentation. The aim of the study was to find out if the Lavandula essential oil has depigmenting properties on human skin, having as theoretical premise its tyrosinase-blocking effect, previously reported in literature for some plant species. By using gas chromatograph coupled with mass spectrometer (GC/MS), a pharmaceutical cream preparation, a protocol of exploration on volunteers (including criteria) and a mexameter, we have highlighted the composition of a sample of Lavandula angustifolia essential oil (LEO) and its depigmenting effect on skin melanic macula. The results highlightes that the main compounds identified were linalool (26.783%), terpinen-4-ol (22.143%) and 3-carene(21.668%), Terpinen-4-ol is represented as the possible active compound in tyrosinase inhibition. Our dermocosmetic assay shows that the depigmenting effect of Lavandula angustifolia oil is effective after 2 months of daily topical treatement, when the melanine average value (measured by the mexameter) decreases more than a third from the innitial value. The study remarks that Lavandula angustifolia essential oil (LEO) in daily cutaneous pharmaceutical form application, during two month, reduces the intensity of spot skin melanin, exprimed through mexametric values.Keywords: Lavandula angustifolia L.; tyrosinase inhibitor; melanic skin macula.

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Section: Chemical Compositionsupporting

confidence: 91%

Chemical Composition and the Potential of Lavandula angustifolia L. Oil as a Skin Depigmentant

Andrei¹,

Alexa²,

Tulcan³

et al. 2018

Rec.Nat.Prod.

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“…Except for linolenic acid, AcerOBP6 could also bind with other plant volatiles strongly, such as 3-carene and ethyl-trans-cinnamate. Studies have reported that 3-carene is the main component of monoterpenes in plant volatile organic compounds and plays a potential role in attracting parasitic wasps [38,39]. Ethyl trans-cinnamate is isolated from lodgepole pine, Pinus contorta, which has been bioassayed as an antifeedant for pine weevils [40] but it is also described to have a sweet honey odor [41].…”

Section: Discussionmentioning

confidence: 99%

Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee

Zhao

Zhu

Huang

et al. 2021

Insects

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Olfaction is essential in some behaviors of honeybee, such as nursing, foraging, attracting a mate, social communication, and kin recognition. OBPs (odorant binding proteins) play a key role in the first step of olfactory perception. Here, we focused on a classic OBP with a PBP-GOBP domain from the Asian honeybee, Apis cerana cerana. Beyond that, the mRNA expression profiles and the binding affinity of AcerOBP6 were researched. According to qRT-PCR analysis, AcerOBP6 transcripts were mainly expressed in the antennae of forager bees. In addition, we found that the expression level of AcerOBP6 was higher than that of AmelOBP6. The fluorescence competitive binding assay indicated that the AcerOBP6 protein had binding affinity with most of the tested odors, including queen pheromone, worker pheromone, and floral volatiles, among which the strongest one was linolenic acid (with a Ki value of 1.67). However, AcerOBP6 was not sensitive to the brood pheromones. A further study based on EAG assay revealed that the antennae had the strongest response to 2-heptanone. The EAG recording values of the selected ligands were all reduced after AcerOBP6 was silenced, with 8 of 14 declining significantly (p < 0.01) given that these odors could specifically bind to AcerOBP6. As revealed in our current study, AcerOBP6 might be a crucial protein involved in olfactory recognition for foraging. Overall, the research provides a foundation for exploring the olfactory mechanism of A. cerana cerana.

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“…So far, molecular genetic research related to Lavandula species has been focused mainly on cloning and characterization of terpene synthase genes involved in biosynthesis of the essential oil compounds [4][5][6][7][8][9] and draft genome sequence [10]. However, molecular marker development and application for characterization of the genetic resources and biodiversity of lavender and other Lavandula species has been restricted to a few reported studies.…”

Section: Introductionmentioning

confidence: 99%

SRAP markers for genetic diversity assessment of lavender (Lavandula angustifolia mill.) varieties and breeding lines

Zagorcheva

Stanev

Rusanov

et al. 2020

Biotechnology & Biotechnological Equipment

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Lavender (Lavandula angustifolia Mill.) is an essential oil-bearing and medicinal plant of great economic and social importance to Bulgaria, which is the world largest producer of lavender oil nowadays. Currently, the industrial cultivation of lavender in Bulgaria employs up to seven varieties established during the last century. Despite the growing industrial lavender cultivation, few molecular markers have been applied for characterization of lavender genetic resources and breeding. The present study employed sequence-related amplified polymorphism (SRAP) markers to characterize the genetic resources and genetic diversity within and between two groups of ten Bulgarian and five foreign lavender varieties and breeding lines. The data generated following analysis with 51 SRAP primer pairs showed amplification of a high number (4697) and ratio (77.2%) of polymorphic SRAP fragments resulting in relatively high value of the polymorphism information content, with an average of 0.27 ± 0.03 for the used primer pairs. Analysis of molecular variance of the SRAP data further revealed high level of genetic diversity (96.6% of the total variations) within the analyzed groups of Bulgarian and foreign varieties and rather low diversity (3.4% of total variations) between the two groups. The possibilities for further applications of SRAP markers in lavender breeding and cultivation are discussed.

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Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia

Cited by 19 publications

References 61 publications

Chemical Composition and the Potential of Lavandula angustifolia L. Oil as a Skin Depigmentant

Chemical Composition and the Potential of Lavandula angustifolia L. Oil as a Skin Depigmentant

Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee

SRAP markers for genetic diversity assessment of lavender (Lavandula angustifolia mill.) varieties and breeding lines

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