ISOLATION AND DETECTION OF <i>HELICOBACTER PYLORI</i> FROM PATIENTS SUFFERING FROM PEPTIC ULCER USING BIOCHEMICAL TESTS AND MOLECULAR TECHNIQUES

Abstract

Helicobacter pylori is the causative agent of most cases of gastritis. There is no established gold standard for the diagnosis of H. pylori infection. A reliable diagnosis is crucial to confirm that eradication therapy has been successful. Eighty gastric biopsy and blood samples were obtained from fasting Jordanian patients with Esophago-Gastro-Doudenoscopy (EGD). Several diagnosis tests for H. pylori infections were used and compared including: Culture, microscopic examination, histopathology, Rapid Urease Test (RUT), serology, biochemical tests, antibiotic susceptibility test and molecular method. Forty two patients were considered H. pylori positive in both histopathology examination and RUT test. On the other hand, 57 patient were detected to have anti-IgA, IgG H. pylori antibody positive by ELISA test. Ten patients had equivocal results but not in both tests. A total of 19 biopsy samples were positive for H. pylori according to culture test. This result was confirmed by endoscopic examination, urease, catalase and oxidase. A high percentages of resistance to vancomycin, polymyxin B and amoxicillin was observed (100, 100 and 94.7%, respectively) with various degree of sensitivity to all of the first line of antibiotics. Molecular technique (PCR) was used to detect CagA gene which appeared positive in 14 patients. We conclude that the histopathology and RUT tests are reliable invasive diagnosis for H. pylori. However, culture test appear to be the most important (if the therapy failed) to detect antibiotic susceptibility to H. pylori strains.

Study of Diagnostic Tests for Helico B Acter Pylori Infection

“…In the present study, Sensitivity of IgG serology is 100% correlating with Nassir E. Mohsun, et al 10 (100%) and Ruba S. Abu-Sbeih et al 12 (100%).…”

“…Sensitivity and Specificity of Rapid urease test was 100% and 73.5% respectively. Sensitivity of this study correlates with Ruba S. Abu-Sbeih et al 12 (100%) and Specificity was nearer to Saffari Mahmood et al 13 …”

Antibacterial Effects of Cinnamon Extract, Clove Oil and Antibiotics against Helicobacter pylori Isolated from Stomach Biopsies

“…Abu-Sbeih and coauthors [22] reported 100% resistance to vancomycin in a similar study conducted in Jordon, these results are in compliance with the results of our study. Whereas another study reported 100 % H. pylori resistance to erythromycin [23] following the same pattern of results reported in our study.…”

Seroprevalence of Helicobacter Pylori Infection among Patients with Gastroduodenal Disorders in Erbil City

“…Epidemiological data on the prevalence of H. pylori infection to be very scattered in most developing countries including Iraq. The prevalence of H. pylori in the neighboring countries was found to be 49.7% in Kuwait [5], 69.0% in Turkey [9], 30.1% in Oman [16], 53% in Yemen [19], 25% in Jordan [3], 71.33% in Saudi Arabia [7], 61.87% in Iran [38], 42.1% in Lebanon (Khalife et al, 2017), and 41% in The United Arab Emirates [33]. As well as, in other countries the prevalence of H. pylori was found to be 56.9% in Kosovo [46], 64.39% in Cameroon [34], 81.7% in Nigeria [28] 47% in Pakistan [39], 27% in India [25] and 24.3% in Uganda [4].…”