Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains

Rodrigues, Luciana Machado; Forte, Bianca de Camargo; Bodanese‐Zanettini, Maria Helena

doi:10.1590/s1516-89132006000500002

Cited by 9 publications

(3 citation statements)

References 23 publications

(24 reference statements)

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“…The Alexander stain showed the highest pollen viability (90.0-97.1%) which was likely an overestimated range in comparison to FCR (83.0%). The highest percentage of viability might be due to the fact that the stain killed the pollen (Parfitt & Ganeshan 1989;Kapyla 1991;Sedgly & Hardarb 1993;Khatum & Flowers 1995), and another drawback associated with these tests based on cellular content and presence of cellular content is that they do not guarantee that pollen is metabolically active or capable of growing pollen tubes (Rodrigues et al 2006). On the contrary, Auler et al (2006) used Alexander's stain in the medicinal species Baccharis trimera ('carqueja') and concluded that Alexander's stain was the best to estimate pollen viability.…”

Section: Discussionmentioning

confidence: 99%

Effect of seasonality and time after anthesis on the viability and longevity ofCannabis sativapollen

Choudhary

Siddiqui

et al. 2014

Palynology

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The aim of this study was to evaluate the pollen viability and longevity of Cannabis sativa, which is a significant economic crop. Viability and longevity are evaluated using different staining techniques: Alexander's, Triphenyl Tetrazolium Chloride (TCT) and Fluorochromatic Reaction (FCR). The results of this study show that FCR is the most reliable and accurate for assessing viability and longevity in C. sativa. Viability was measured over days and across 2010. Viability was greatest between 0 -3 days of anther dehiscence and during April, whilst December had the lowest pollen viability. For Cannabis sativa the first 3 days after anthesis are the most important for fertilization, following the 3rd day there is a rapid decline in viability.

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Section: Discussionmentioning

confidence: 99%

Effect of seasonality and time after anthesis on the viability and longevity ofCannabis sativapollen

Choudhary

Siddiqui

et al. 2014

Palynology

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“…Fenomena di atas umumnya telah dilaporkan oleh banyak peneliti atau kelompok penelit pada berbagai tanaman budidya. Tanaman-tanaman dimaksud seperti tanaman tembakau (Volcov et al, 2005), Brassica (Zhang et al, 2006), kedelai (Rodrigues et al, 2006), tomat (Segui-Semarro dan Nuez, 2007), dan kentang (Panahandeh et al, 2008). Bagi tanaman jarak pagar, selain morfologi bunga berupa panjang dan lebar bunga, umur bunga juga menentukan frekuensi mikrospora uninukleat tengah dan akhir.…”

Section: E F Pembahasanunclassified

MORFOLOGI BUNGA YANG SESUAI BAGI KULTUR MIKROSPORA PADA TANAMAN JARAK PAGAR (Jatropha curcas L.)

Suaib¹,

Arma²,

Muhidin³

2015

Agroteknos

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An experiment aimed in gaining information on morphological characteristics of Jatropha curcas (L.) containing high frequency of uninucleate microspores has been done. This was important due to the successful application of experiment in haploid technique through anther or microspore culture is strongly determined by the availability of information from basic research such as appropriate morphology of the flowers. This scientific article, in our knowledge, was an early explanation of the initial steps of whole procedures in the microspore culture of Jatropha curcas (L.). Results of the research showed that the morphological features of flower that contained high frequency of mid- and late uninucleate microspores was that the flowers must be in the range of 2 to 3 mm in length. Such flowers contained yellowish anthers.Key words: Flowers length, mid- and late-uninucleate, morphology, physic nut, microspore

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“…Soybean [ Glycine max (L.) Merrill, Fabaceae; 2n = 4x = 40] is a self-pollinated crop. It is a multifaced nutritional food crop with high amounts of proteins (40%), fats (20%), oil contents and as a medicinal crop ( Hymowitz, 1970 ; Singh et al, 2001 ; Rodrigues et al, 2006 ; Medic et al, 2014 ; Rajendran and Lal, 2020 ; Rajendran et al, 2022 ; Ramlal et al, 2022 ). Soybean is a paleopolyploid which has resulted in the presence of more than one copy of a gene in about 75% of its genes and shows differential expression ( Friedrichs et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Harnessing heterosis and male sterility in soybean [Glycine max (L.) Merrill]: A critical revisit

et al. 2022

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Soybean is a predominantly self-pollinated crop. It is also one of the important oilseed legumes. Soybean is an excellent crop having industrial, traditional, culinary, feeding, and cultural roles. Genetic diversity in breeding programs is of prime importance as it ensures the success of any breeding by enhancing the outcomes and results of the plants. The phenomenon wherein the progeny exhibits greater biomass (yield) and a faster rate of development and fertility than its parents is referred to as heterosis. As of now, heterosis is mainly limited to the trait of seed yield and is considered the basis for the development of better (superior) varieties. Male sterility (MS) is extensively used for the production of seeds and the improvement of crops coupled with the traditional breeding programs and molecular technology. Therefore, deployment of MS and heterosis in breeding soybean could yield better outcomes. This review aims to focus on two aspects, namely, MS and heterosis in soybean with its scope for crop improvement.

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Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains

Abstract: ABSTRACT

Cited by 9 publications

References 23 publications

Effect of seasonality and time after anthesis on the viability and longevity ofCannabis sativapollen

Effect of seasonality and time after anthesis on the viability and longevity ofCannabis sativapollen

MORFOLOGI BUNGA YANG SESUAI BAGI KULTUR MIKROSPORA PADA TANAMAN JARAK PAGAR (Jatropha curcas L.)

Harnessing heterosis and male sterility in soybean [Glycine max (L.) Merrill]: A critical revisit

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