Harnessing heterosis and male sterility in soybean [Glycine max (L.) Merrill]: A critical revisit

Ramlal, Ayyagari; Nautiyal, Aparna; Baweja, Pooja; Mahto, Rohit Kumar; Mehta, Sahil; Mallikarunja, Bingi Pujari; Vijayan, Roshni; Saluja, Shukla; Kumar, Vijay; Dhiman, Sunil; Lal, S. K.; Raju, Dhandapani; Rajendran, Ambika

doi:10.3389/fpls.2022.981768

Cited by 12 publications

(11 citation statements)

References 89 publications

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“…Soybean is widely grown and consumed globally and constitutes nearly 28% of vegetable oil and 70% of protein meals worldwide ( SoyStats, 2021 ). Because of its great nutritional content, it is used in both human food and animal feed ( Ramlal et al., 2022a ; 2022b ). Besides, it is used in the pharmaceutical and cosmetic industries.…”

Section: Introductionmentioning

confidence: 99%

Identification of quantitative trait loci (QTLs) and candidate genes for seed shape and 100-seed weight in soybean [Glycine max (L.) Merr.]

et al. 2023

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Seed size and shape are important traits determining yield and quality in soybean. Seed size and shape are also desirable for specialty soy foods like tofu, natto, miso, and edamame. In order to find stable quantitative trait loci (QTLs) and candidate genes for seed shape and 100-seed weight, the current study used vegetable type and seed soybean-derived F2 and F2:3 mapping populations. A total of 42 QTLs were mapped, which were dispersed across 13 chromosomes. Of these, seven were determined to be stable QTLs and five of them were major QTLs, namely qSL-10-1, qSW-4-1, qSV-4-1, qSLW-10-1, and qSLH-10-1. Thirteen of the 42 QTLs detected in the current study were found at known loci, while the remaining 29 were discovered for the first time. Out of these 29 novel QTLs, 17 were major QTLs. Based on Protein Analysis Through Evolutionary Relationships (PANTHER), gene annotation information, and literature search, 66 genes within seven stable QTLs were predicted to be possible candidate genes that might regulate seed shape and seed weight in soybean. The current study identified the key candidate genes and quantitative trait loci (QTLs) controlling soybean seed shape and weight, and these results will be very helpful in marker-assisted breeding for developing soybean varieties with improved seed weight and desired seed shape.

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Section: Introductionmentioning

confidence: 99%

Identification of quantitative trait loci (QTLs) and candidate genes for seed shape and 100-seed weight in soybean [Glycine max (L.) Merr.]

et al. 2023

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“…One approach to increase outcrossing without emasculating flowers is to block selffertilization using male sterility (MS). The combination of nuclear male sterility (NMS), cytoplasmic male sterility (CMS), and photoperiod/temperature-sensitive genic male sterility systems with fertility restorer genes have produced promising results in small trials of soybean hybrids (Nadeem et al 2021;Ramlal et al 2022;Palmer 2000;Thu et al 2019;Palmer et al 2001). However, multiple drawbacks exist in these systems, making them near impractical for commercial hybrid soybean breeding applications.…”

Section: Introductionmentioning

confidence: 99%

Introduction of Barnase/Barstar in soybean produces a rescuable male sterility system for hybrid breeding

Szeluga

Baldrich

DelPercio

et al. 2023

Preprint

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Hybrid breeding for increased vigor has been used for over a century to boost agricultural outputs without requiring higher inputs. While this approach has led to some of the most substantial gains in crop productivity, breeding barriers have fundamentally limited soybean (Glycine max) from reaping the benefits of hybrid vigor. Soybean makes inconspicuous flowers that self-fertilize before opening, and thus are not readily amenable to outcrossing. In this study, we demonstrate that the Barnase/Barstar male sterility/male rescue system can be used in soybean to produce hybrid seeds. By expressing the cytotoxic ribonuclease, Barnase, under a tapetum-specific promoter in soybean anthers, we are able to completely block pollen maturation, creating male-sterile plants. We show that fertility can be rescued in the F1 generation of these Barnase-expressing lines when they are crossed with pollen from plants that express the Barnase inhibitor, Barstar. Importantly, we found that the successful rescue of male fertility is dependent on the relative dosage of Barnase and Barstar. When Barnase and Barstar were expressed under the same tapetum-specific promoter, the F1 offspring remained male-sterile. When we expressed Barstar under a relatively stronger promoter than Barnase, we were able to achieve a successful rescue of male fertility in the F1 generation. This work demonstrates the successful implementation of a biotechnology approach to produce fertile hybrid offspring in soybean.

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“…One approach to increase outcrossing without emasculating flowers is to block self‐fertilization using male sterility (MS). The combination of nuclear male sterility (NMS), cytoplasmic male sterility (CMS) and photoperiod/temperature‐sensitive genic male sterility systems with fertility restorer genes have produced promising results in small trials of soybean hybrids (Nadeem et al ., 2021; Palmer, 2000; Palmer et al ., 2001; Ramlal et al ., 2022; Thu et al ., 2019). However, multiple drawbacks exist in these systems, making them nearly impractical for commercial hybrid soybean breeding applications.…”

Section: Introductionmentioning

confidence: 99%

“…Fertility is restored in the second generation by crossing barnase-expressing plants with pollen carrying a transgene for the tapetum-specific expression of the ribonuclease inhibitor, barstar (Hartley, 1988(Hartley, , 1989(Hartley, , 2001Mariani et al, 1990Mariani et al, , 1992. This approach has been adopted to create hybrid breeding systems for several crops, including a variety of vegetables and oil seed crops (Colombo and Galmarini, 2017;Jagannath et al, 2002;Mariani et al, 1992;Ray et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Introduction of barnase/barstar in soybean produces a rescuable male sterility system for hybrid breeding

Szeluga,

Baldrich,

DelPercio

et al. 2023

Plant Biotechnology Journal

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SummaryHybrid breeding for increased vigour has been used for over a century to boost agricultural outputs without requiring higher inputs. While this approach has led to some of the most substantial gains in crop productivity, breeding barriers have fundamentally limited soybean (Glycine max) from reaping the benefits of hybrid vigour. Soybean flowers self‐pollinate prior to opening and thus are not readily amenable to outcrossing. In this study, we demonstrate that the barnase/barstar male sterility/rescue system can be used in soybean to produce hybrid seeds. By expressing the cytotoxic ribonuclease, barnase, under a tapetum‐specific promoter in soybean anthers, we are able to completely block pollen maturation, creating male sterile plants. We show that fertility can be rescued in the F1 generation of these barnase‐expressing lines when they are crossed with pollen from plants that express the barnase inhibitor, barstar. Importantly, we found that the successful rescue of male fertility is dependent on the relative dosage of barnase and barstar. When barnase and barstar were expressed under the same tapetum‐specific promoter, the F1 offspring remained male sterile. When we expressed barstar under a relatively stronger promoter than barnase, we were able to achieve a successful rescue of male fertility in the F1 generation. This work demonstrates the successful implementation of a biotechnology approach to produce fertile hybrid offspring in soybean.

show abstract

Harnessing heterosis and male sterility in soybean [Glycine max (L.) Merrill]: A critical revisit

Cited by 12 publications

References 89 publications

Identification of quantitative trait loci (QTLs) and candidate genes for seed shape and 100-seed weight in soybean [Glycine max (L.) Merr.]

Identification of quantitative trait loci (QTLs) and candidate genes for seed shape and 100-seed weight in soybean [Glycine max (L.) Merr.]

Introduction of Barnase/Barstar in soybean produces a rescuable male sterility system for hybrid breeding

Introduction of barnase/barstar in soybean produces a rescuable male sterility system for hybrid breeding

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