Effect of seasonality and time after anthesis on the viability and longevity of<i>Cannabis sativa</i>pollen

Choudhary, Namrta; Siddiqui, M. B.; Bi, Shazia; Khatoon, Sayyada

doi:10.1080/01916122.2014.892906

Cited by 15 publications

(14 citation statements)

References 41 publications

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“…As a result of a week-long cold pretreatment applied directly on excised buds prior to microspore culture, a stress-derived slight decrease in microspore viability was detected. Our findings are in agreement with results published by Choudhary et al (2014) , who studied how pollen viability was significantly influenced by seasonal fluctuations in temperature and humidity. Researchers reported a significant decrease of pollen viability at low temperatures, reaching the lowest viability rates in the winter season.…”

Section: Discussionsupporting

confidence: 93%

“…The great amount of microspores and pollen grains produced in each flower bud (>150,000), together with the high rates of microspore viability reached for all phenotypes (>50%), makes C. sativa a suitable system able to provide, in a very quick period of time (≈30 days after seed germination), huge populations of viable vacuolate microspores and young bi-cellular pollen grains potentially inducible to afford microspore and pollen embryogenesis experiments. Viability rates obtained in our research are similar to the ones reported by Choudhary et al (2014) which, depending on the season, oscillated among 33.3 and 83.0%, while in another work (Zottini et al, 1997) a viability rate for mature pollen grains of 92% was reported. This difference among viability rates could be attributable to the microspore isolation procedure, as viability measurements in the former works were performed on mature pollen grains naturally released after anthesis.…”

Section: Cannabis Sativa Is a Suitable Candidate For Androgenesis Experiments Mainly Due To Its Fast Production Of Viable And Potentiallysupporting

confidence: 89%

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Microgametophyte Development in Cannabis sativa L. and First Androgenesis Induction Through Microspore Embryogenesis

Galán-Ávila¹,

García‐Fortea

Prohens

et al. 2021

Front. Plant Sci.

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Development of double haploids is an elusive current breeding objective in Cannabis sativa L. We have studied the whole process of anther and pollen grain formation during meiosis, microsporogenesis, and microgametogenesis and correlated the different microgametophyte developmental stages with bud length in plants from varieties USO31 and Finola. We also studied microspore and pollen amyloplast content and studied the effect of a cold pretreatment to excised buds prior to microspore in vitro culture. Up to 476,903 microspores and pollen grains per male flower, with in vivo microspore viability rates from 53.71 to 70.88% were found. A high uniformity in the developmental stage of microspores and pollen grains contained in anthers was observed, and this allowed the identification of bud length intervals containing mostly vacuolate microspores and young bi-cellular pollen grains. The starch presence in C. sativa microspores and pollen grains follows a similar pattern to that observed in species recalcitrant to androgenesis. Although at a low frequency, cold-shock pretreatment applied on buds can deviate the naturally occurring gametophytic pathway toward an embryogenic development. This represents the first report concerning androgenesis induction in C. sativa, which lays the foundations for double haploid research in this species.

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Section: Discussionsupporting

confidence: 93%

Section: Cannabis Sativa Is a Suitable Candidate For Androgenesis Experiments Mainly Due To Its Fast Production Of Viable And Potentiallysupporting

confidence: 89%

Microgametophyte Development in Cannabis sativa L. and First Androgenesis Induction Through Microspore Embryogenesis

Galán-Ávila¹,

García‐Fortea

Prohens

et al. 2021

Front. Plant Sci.

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“…A.M.U/31337 (male), 31338 (female)/2010 were deposited in Botany Department, Aligarh Muslim University, Aligarh for future reference. Monthly mean maximum and mean minimum temperature and relative humidity were selected for this study [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Impact of Seasons and Dioecy on Therapeutic Phytoconstituents ofTinospora cordifolia, a Rasayana Drug

Choudhry

Singh

Siddiqui

et al. 2014

BioMed Research International

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Tinospora cordifolia (Thunb.) Miers, Menispermaceae, is a dioecious creeper, commonly known as “Giloe” or “Guduchi” with significant medicinal importance in the traditional systems of medicine. It is designated as Rasayana drug in Ayurveda and recommended for a number of diseases and also as adaptogen and immunomodulator. The safety and efficacy of herbal medicines are closely correlated with the quality of the source materials. The aim of this study is to see the effect of seasons on phytoconstituents and how these vary in male and female stem samples of T. cordifolia. The study revealed that total phenolics and total sugar concentration obtained highest values in summer season while starch and tannin content were found maximum in winter season in both the genders. However, biomarkers, tinosporaside and berberine, reached to their highest concentration in monsoon season. Further, antioxidant potential revealed the highest inhibition percentage in winter season as well as in late summer season. The results of this study suggest that the female plant is best for its therapeutic phytoconstituents and the best harvesting seasons may be either winter or late summer for antioxidant potential and immunomodulator activities and monsoon for antidiabetic activity of T. cordifolia.

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“…Pollen viability was examined using fluorescein diacetate (FDA) and pollen germination (Heslop‐Harrison and Heslop‐Harrison, ; Djanaguiraman et al., ; Choudhary et al., ). Pollen enzyme activity was assessed by performing a fluorochromatic reaction with 0.05 mg mL −1 FDA which enters the pollen wall and is hydrolyzed by esterase (Choudhary et al., ). Mature, non‐dehisced anthers were removed from three buds per plant, stained in FDA, and observed under a fluorescent microscope with a fluorescein isothiocyanate (FITC) filter.…”

Section: Methodsmentioning

confidence: 99%

Reproductive heat tolerance in a Mojave Desert annual plant,Trianthema portulacastrum

Branch

Sage

2018

American J of Botany

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Premise of the Study Reproduction in many crop species is impaired above 30° to 35°C; however, the sensitivity of reproduction in the natural flora remains uncertain. Studies focusing on the effect of high temperature on plant reproduction in wild species are necessary to improve our understanding of how rising global temperatures will impact global plant reproductive success and may ultimately inform models of plant distribution in the future. Additionally, these studies may highlight candidates for thermotolerance that could be further explored for crop improvement. Methods We studied reproductive heat tolerance in Trianthema portulacastrum, a weedy species found in hot microsites throughout the tropics and subtropics. Plants were grown at seven day/night temperature combinations: 30°/24°C, 33°/24°C, 36°/24°C, 40°/24°C, 44°/24°C, 24°/40°C, and 40°/40°C to study the effect of both high‐day and high‐night temperatures. The reproductive parameters measured include anther dehiscence, pollen viability, germination, ovule number, and seed set. Key Results Pollen viability and germination declined with increasing daytime temperature up to 44°C, but this did not affect fruit production or seed set. Seed set was reduced under high night temperature. Continuous high temperature over the day and night (40°C day/40°C night) decreased pollen viability by half and reduced seed set by two‐thirds. Conclusions Our results demonstrate Trianthema portulacastrum has much higher reproductive thermotolerance than commonly identified in crop species, and though inhibited, can retain fecundity at 40°C. Through a combination of night escape and increased thermal tolerance, it maintains fertility in the hot microsites of its natural environment.

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Effect of seasonality and time after anthesis on the viability and longevity ofCannabis sativapollen

Cited by 15 publications

References 41 publications

Microgametophyte Development in Cannabis sativa L. and First Androgenesis Induction Through Microspore Embryogenesis

Microgametophyte Development in Cannabis sativa L. and First Androgenesis Induction Through Microspore Embryogenesis

Impact of Seasons and Dioecy on Therapeutic Phytoconstituents ofTinospora cordifolia, a Rasayana Drug

Reproductive heat tolerance in a Mojave Desert annual plant,Trianthema portulacastrum

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