Isolation and characterization of the major nod factor of Bradyrhizobium japonicum strain 532C

Soulemanov, A.; Prithiviraj, Balakrishnan; Carlson, Russell W.; Jeyaretnam, Benjamin; Smith, Donald L.

doi:10.1078/0944-5013-00128

Cited by 24 publications

(17 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fig. 2a) was conducted as described by Prithiviraj et al (2000) and Souleimanov et al (2002a). B. japonicum (strain 532C) was grown in yeast mannitol medium (YEM) at 28°C with constant shaking at 150 rpm.…”

Section: Methodsmentioning

confidence: 99%

Response of Arabidopsis thaliana roots to lipo-chitooligosaccharide from Bradyrhizobium japonicum and other chitin-like compounds

et al. 2010

View full text Add to dashboard Cite

Lipo-chitooligosaccharides (LCOs) are bacteriato-plant signals required for the establishment of rhizobialegume nitrogen fixing symbioses. The ability of LCO [Nod Bj V (C 18:1 , MeFuc)] isolated from B. japonicum (strain 532C), and of oligomers of chitosan (tetramer, pentamer) and chitin (pentamer) to affect the developmental morphology of roots in Arabidopsis thaliana (L.) Heynh ecotype Columbia (Col-0) was assessed using an interactive scannerbased image analysis system. LCOs have been shown to play a role in plant organogenesis at nanomolar concentrations. LCO and the chitin pentamer promoted root growth and development in Arabidopsis at concentrations of 10 nM and 100 lM, respectively. The LCO treated Arabidopsis plants had about 35% longer roots than untreated control plants. Similarly, treatment with 100 lM chitin pentamer (CHIT5) resulted in 26% longer roots than the untreated plants; however, chitosan oligomer (CH4 or CH5) treated plants did not differ from the control plants at either concentration (100 or 1 lM). Both LCOs and the chitin pentamer at higher concentrations increased root surface area, mean root diameter and number of root tips. However, leaf area increase was observed only in plants treated with LCO at 10 nM.

show abstract

Section: Methodsmentioning

confidence: 99%

Response of Arabidopsis thaliana roots to lipo-chitooligosaccharide from Bradyrhizobium japonicum and other chitin-like compounds

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Most contained six GlcN residues substituted with acetyl or various C 16 -C 18 acyl groups. Nod factors from Bradyrhizobium japonicum strain 532C, a bacterium that nodulates soybean effectively in the cool Canadian spring, has been found by FAB and MALDI (DHB) to be substituted with methyl-fucose and the fatty acid, 18:1 (Soulemanov et al, 2002). Increasing interest in biopharmaceuticals has prompted investigations into large-scale production in transgenic plants.…”

Section: Nod Factors From Rhizobial Bacteriamentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

Harvey¹

2008

Mass Spectrometry Reviews

View full text Add to dashboard Cite

This review is the second update of the original review on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates that was published in 1999. It covers fundamental aspects of the technique as applied to carbohydrates, fragmentation of carbohydrates, studies of specific carbohydrate types such as those from plant cell walls and those attached to proteins and lipids, studies of glycosyl-transferases and glycosidases, and studies where MALDI has been used to monitor products of chemical synthesis. Use of the technique shows a steady annual increase at the expense of older techniques such as FAB. There is an increasing emphasis on its use for examination of biological systems rather than on studies of fundamental aspects and method development and this is reflected by much of the work on applications appearing in tabular form.

show abstract

“…Production of lipo-chitooligosaccharide Lipo-chitooligosaccharide was produced according to the method described in Souleimanov et al (2002).…”

Section: Methodsmentioning

confidence: 99%

The response of canola cultivars to lipo-chitooligosaccharide (Nod Bj V [C18:1, MeFuc]) and thuricin 17

et al. 2015

View full text Add to dashboard Cite

Lipo-chitooligosaccharides (LCOs) are signal molecules that play a role in initiating the symbiotic legume-rhizobia and arbuscular mycorrhizal processes. Thuricin 17 is a bacteriocin produced by Bacillus thuringiensis strain NEB 17. Previous work has shown that the application of a 10 -6 M LCO solution, or a 10 -11 M thuricin 17 solution, can stimulate the germination and growth of a wide range of plants. The objectives were: to grow canola cultivar 04C111 under optimal temperature conditions in a greenhouse, and to assess the effect of LCO seed application and spray treatment on 04C111 floral initiation, architecture, and factors that contribute to yield; to assess the effect of a thuricin 17 seed application treatment upon factors that contribute to 04C111 yield in a stressfully high temperature greenhouse system; and to assess the effect of an LCO seed application and spray treatment on Topas floral initiation, architecture, and factors that contribute to yield, and seed quality, in a simulated ''cool spring'' greenhouse system. According to the 95 % confidence limits, Brassica napus cultivars grown from untreated seed produced 39 % fewer to 1 % fewer branches than plants grown from LCO-treated seed. Application of an LCO foliar spray increased the total of branch lengths per 04C111 plant by 16.93 ± 8.41 cm. Based on the stem-to-branch length ratio, LCO-sprayed 04C111 plants were 68 % less apically dominant than unsprayed plants. Topas plants that were sprayed with LCO produced seed with approximately 4.45 ± 0.23 % more oil (% d.w.) than unsprayed plants. For both cultivars, seed treatment with 10 -6 M of LCO produced plants with a higher average sum of primary, secondary, and tertiary branch (C1 cm) frequencies.

show abstract

Isolation and characterization of the major nod factor of Bradyrhizobium japonicum strain 532C

Cited by 24 publications

References 6 publications

Response of Arabidopsis thaliana roots to lipo-chitooligosaccharide from Bradyrhizobium japonicum and other chitin-like compounds

Response of Arabidopsis thaliana roots to lipo-chitooligosaccharide from Bradyrhizobium japonicum and other chitin-like compounds

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

The response of canola cultivars to lipo-chitooligosaccharide (Nod Bj V [C18:1, MeFuc]) and thuricin 17

Contact Info

Product

Resources

About