The response of canola cultivars to lipo-chitooligosaccharide (Nod Bj V [C18:1, MeFuc]) and thuricin 17

Abstract

Lipo-chitooligosaccharides (LCOs) are signal molecules that play a role in initiating the symbiotic legume-rhizobia and arbuscular mycorrhizal processes. Thuricin 17 is a bacteriocin produced by Bacillus thuringiensis strain NEB 17. Previous work has shown that the application of a 10 -6 M LCO solution, or a 10 -11 M thuricin 17 solution, can stimulate the germination and growth of a wide range of plants. The objectives were: to grow canola cultivar 04C111 under optimal temperature conditions in a greenhouse, and to assess the effect of LCO seed application and spray treatment on 04C111 floral initiation, architecture, and factors that contribute to yield; to assess the effect of a thuricin 17 seed application treatment upon factors that contribute to 04C111 yield in a stressfully high temperature greenhouse system; and to assess the effect of an LCO seed application and spray treatment on Topas floral initiation, architecture, and factors that contribute to yield, and seed quality, in a simulated ''cool spring'' greenhouse system. According to the 95 % confidence limits, Brassica napus cultivars grown from untreated seed produced 39 % fewer to 1 % fewer branches than plants grown from LCO-treated seed. Application of an LCO foliar spray increased the total of branch lengths per 04C111 plant by 16.93 ± 8.41 cm. Based on the stem-to-branch length ratio, LCO-sprayed 04C111 plants were 68 % less apically dominant than unsprayed plants. Topas plants that were sprayed with LCO produced seed with approximately 4.45 ± 0.23 % more oil (% d.w.) than unsprayed plants. For both cultivars, seed treatment with 10 -6 M of LCO produced plants with a higher average sum of primary, secondary, and tertiary branch (C1 cm) frequencies.

A PGPR-Produced Bacteriocin for Sustainable Agriculture: A Review of Thuricin 17 Characteristics and Applications

“…Only canola seeds treated with thuricin 17 developed roots under very stressfully low temperature (10/4°C) and salt stress conditions ( Schwinghamer et al, 2016a ). Likewise, plants treated with thuricin 17 produced one more leaf per plant than the control treatment and other biostimulant treatments at 30/30°C, which is very stressful for a temperate zone crop such as canola ( Schwinghamer et al, 2016b ). Rosettes of Arabidopsis thaliana Col-0 treated with thuricin 17 had decreased levels of cytokinins, gibberellins, jasmonic acid, and abscisic acid at 24 h after treatment with thuricin 17, and increased levels of indole-3-acetic acid (IAA; 85%) and salicylic acid (SA; 42%) compared to controls ( Subramanian, 2014 ).…”

Microbial Derived Compounds Are a Promising Approach to Mitigating Salinity Stress in Agricultural Crops

“…Arabidopsis thaliana treated with thuricin 17 tolerated up to 250 mM NaCl (Subramanian, 2014). Canola plants treated with 0.2 M NaCl and 10 −9 M thuricin 17 were taller than plants treated with just 0.2 M NaCl (Schwinghamer et al, 2016).…”

Microbial Derived Compounds, a Step Toward Enhancing Microbial Inoculants Technology for Sustainable Agriculture

“… Zhao et al (2020) reported enhanced tolerance to salt by Arabidopsis thaliana plants treated with N-3-oxo-hexanoyl-homoserine lactone. Regulation of biomass and leaf arrangement in drought stressed Brassica napus [L.], treated with LCO was observed ( Schwinghamer et al, 2016 ). Subramanian et al (2016) observed an increase in germination percentage of soybean seeds exposed to salinity, somewhat similar to non-stressed seeds, following treatment with LCO and thuricin17.…”