Isolation and characterization of simple sequence repeat loci in <i>Rubus hochstetterorum</i> and their use in other species from the Rosaceae family

Lopes, M. S.; Maciel, M. Graciete Belo; Mendonça, Duarte; Gil, Fabíola Sabino; Machado, Artur da Câmara

doi:10.1111/j.1471-8286.2006.01329.x

Cited by 25 publications

(12 citation statements)

References 12 publications

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“…Rubus SSR primer sequences were selected from published reports in red raspberry (Graham et al 2004) and blackberry (Castillo et al 2010;Lopes et al 2006;Lewers et al 2008). Dossett et al (2010) described the transferability of many of these Rubus SSR primers to black raspberry.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Microsatellite markers have been developed from expressed sequence tag (EST) and genomic libraries in red raspberry (Rubus idaeus L.; Castillo et al 2010;Graham et al 2004) and blackberry (Rubus L. subgenus Rubus) (Amsellem et al 2001;Castillo et al 2010;Lewers et al 2008;Lopes et al 2006). More recently, work to develop SSRs from black raspberry ESTs is underway (unpublished data).…”

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confidence: 99%

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Genetic diversity in wild and cultivated black raspberry (Rubus occidentalis L.) evaluated by simple sequence repeat markers

Dossett

Bassil

Lewers

et al. 2012

Genet Resour Crop Evol

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Breeding progress in black raspberry (Rubus occidentalis L.) has been limited by a lack of genetic diversity in elite germplasm. Black raspberry cultivars have been noted for showing very few phenotypic differences and seedlings from crosses between cultivars for a lack of segregation for important traits. Despite these challenges, little molecular work has been done to explore genetic diversity and relationships in wild and cultivated black raspberry germplasm. Microsatellite, or simple sequence repeat (SSR), markers are highly polymorphic codominant markers useful for studying genetic diversity, population genetics, genetic fingerprinting and other applications. We examined genetic diversity in 148 wild and cultivated black raspberry accessions using 21 polymorphic SSR markers. Black raspberry cultivars clustered tightly and showed higher than expected heterozygosity while that of wild accessions was low. Relationships between wild black raspberry accessions were poorly resolved and regional clusters were mostly absent from our analysis. Our results indicated that wild black raspberry germplasm is a relatively untapped resource available for future breeding.

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Section: Plant Materialsmentioning

confidence: 99%

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confidence: 99%

Genetic diversity in wild and cultivated black raspberry (Rubus occidentalis L.) evaluated by simple sequence repeat markers

Dossett

Bassil

Lewers

et al. 2012

Genet Resour Crop Evol

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“…Microsatellite markers RUB126, RUB262, RUB26 (Graham et al, 2002), RhCBA6, RhCBA14, RhCBA15, RhCBA23 and RhCBA28 (Lopes et al, 2006) were used to genotype field and cultivated samples on an ABI 3100 Genetic Analyzer (Life Technologies Corporation, Carlsbad, CA, USA). Annealing temperatures were 54 1C for RhCBA15, 66 1C for RhCBA6 and 59 1C for all others.…”

Section: Dna Extraction and Molecular Markersmentioning

confidence: 99%

Spontaneous hybrids between native and exotic Rubus in the Western United States produce offspring both by apomixis and by sexual recombination

Clark

Jasieniuk

2012

Heredity

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Facultative asexual reproduction is a trait commonly found in invasive species. With a combination of sexual and asexual reproductive modes, such species may adapt to new environments via sexual recombination during range expansion, while at the same time having the benefits of asexuality such as the maintenance of fitness effects that depend upon heterozygosity. In the Western United States, native species of Rubus (Rosaceae) reproduce sexually whereas exotic naturalized Rubus species reproduce by pseudogamous apomixis. We hypothesized that new asexual lineages of Rubus could arise from hybridization in this range. To detect hybridization between native and exotic Rubus, we genotyped 579 individuals collected across California, Oregon and Washington with eight nuclear microsatellites and two chloroplast markers. Principal Coordinate Analysis and Bayesian clustering revealed a limited amount of hybridization of the native R. ursinus with the exotic R. armeniacus and R. pensilvanicus, as well as cultivated varieties. Genetic distances between these hybrids and their offspring indicated that both R. ursinus Â R. armeniacus and R. ursinus Â R. pensilvanicus produced a mix of apomictic and sexual seeds, with sexual seeds being more viable. Although neither of these hybrid types is currently considered invasive, they model the early stages of evolution of new invasive lineages, given the potential for fixed heterosis and the generation of novel genotypes. The hybrids also retain the ability to increase their fitness via sexual recombination and natural selection. Mixed reproductive systems such as those described here may be an important step in the evolution of asexual invasive species.

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“…Each marker system has its own strengths and limitations, making the choice of marker an important decision. Strawberries have been extensively analyzed for clone identification, mapping and diversity studies using randomly amplified polymorphic DNA (RAPD) markers (Hancock et al 1994;Graham et al 1996;Harrison et al 2000;Degani et al 2001;Sugimoto et al 2005), amplified fragment-length polymorphisms (AFLPs) (Degani et al 2001;Tyrka et al 2002) and the simple sequence repeats (SSRs) (Ashley et al 2003;Nourse et al 2002;Cipriani and Testolin 2004;Folta et al 2005;Lewers 2005;Cipriani et al 2006;Lopes et al 2006;Monfort et al 2006;Sargent et al 2006). GilAriza et al (2006) and Keniry et al (2006) described expressed sequence tags (ESTs)-derived microsatellites from cultivated strawberry and their potential use for varietal identification and diversity study.…”

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confidence: 99%

Inter Simple Sequence Repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes

Debnath¹,

Khanizadeh²,

Jamieson³

et al. 2008

Can. J. Plant Sci.

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markers to assess genetic diversity and relatedness within strawberry genotypes. Can. J. Plant Sci. 88: 313Á322. The goal of this study was to determine the level of genetic diversity and relatedness among 16 strawberry (Fragaria)ananassa Duch.) cultivars and 11 breeding lines developed in Canada, using Inter Simple Sequence Repeat (ISSR) markers. Seventeen primers generated 225 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 63 to 77% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.4% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among strawberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in current strawberry breeding programs.Key words: Fragaria)ananassa, DNA fingerprinting, multivariate analysis, breeding, genetic similarity Debnath, S. C., Khanizadeh, S., Jamieson, A. R. and Kempler, C. 2008. É valuation de la diversite´et des liens ge´ne´tiques des ge´notypes de fraisier au moyen de marqueurs inter-microsatellitaires (ISSR). Can. J. Plant Sci. 88: 313Á322. L'e´tude devait e´tablir l'importance de la diversite´et des liens ge´ne´tiques entre 16 cultivars et souches ge´ne´alogiques de fraisier (FragariaÁ ananassa Duch.) cre´e´s au Canada graˆce a`des marqueurs ISSR (Inter Simple Sequence Repeat, fragments intermicrosatellitaires). Dix-sept amorces ont produit 225 bandes polymorphes ISSR-PCR. L'analyse des grappes par la me´thode des groupes-paires non ponde´re´s avec moyenne arithme´tique (UPGMA) re´ve`le une importante similitude ge´ne´tique entre les ge´notypes (de 63 % a`77 %), ce qui concorde avec les re´sultats de l'analyse en composantes principales (PCO). La re´partition ge´ographique des emplacements du programme d'ame´lioration n'explique que 1,4 % de la variation globale, ainsi que l'indique l'analyse de la variance mole´culaire (AMOVA). Les marqueurs ISSR de´tectent un degreś uffisant de polymorphisme pour qu'on puisse diffe´rencier le ge´notype des fraisiers, d'ou`l'utilite´de la technologie pour identifier les cultivars et permettre un meilleur choix des ligne´es parentales dans les programmes actuels d'hybridation du fraisier.Mots clé s: FragariaÁananassa, identification par le code ge´ne´tique, analyse a`variables multiples, hybridation, similitude ge´ne´tiqueThe cultivated strawberry (Fragaria )ananassa Duch.), an octoploid (2n 056) hybrid between the Scarlet or Virginia strawberry (F. virginiana Duch.) and the pistillate South American F. chiloensis (L.) Duch., is a dicotyledonous, perennial low-growing herb grown in most arable regions of the World. The crop is enjoyed by millions of people in all kinds of climates including temperate, Mediterranean, subtropical and tai...

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Isolation and characterization of simple sequence repeat loci in Rubus hochstetterorum and their use in other species from the Rosaceae family

Cited by 25 publications

References 12 publications

Genetic diversity in wild and cultivated black raspberry (Rubus occidentalis L.) evaluated by simple sequence repeat markers

Genetic diversity in wild and cultivated black raspberry (Rubus occidentalis L.) evaluated by simple sequence repeat markers

Spontaneous hybrids between native and exotic Rubus in the Western United States produce offspring both by apomixis and by sexual recombination

Inter Simple Sequence Repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes

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