Inter Simple Sequence Repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes

Abstract

markers to assess genetic diversity and relatedness within strawberry genotypes. Can. J. Plant Sci. 88: 313Á322. The goal of this study was to determine the level of genetic diversity and relatedness among 16 strawberry (Fragaria)ananassa Duch.) cultivars and 11 breeding lines developed in Canada, using Inter Simple Sequence Repeat (ISSR) markers. Seventeen primers generated 225 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 63 to 77% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.4% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among strawberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in current strawberry breeding programs.Key words: Fragaria)ananassa, DNA fingerprinting, multivariate analysis, breeding, genetic similarity Debnath, S. C., Khanizadeh, S., Jamieson, A. R. and Kempler, C. 2008. É valuation de la diversite´et des liens ge´ne´tiques des ge´notypes de fraisier au moyen de marqueurs inter-microsatellitaires (ISSR). Can. J. Plant Sci. 88: 313Á322. L'e´tude devait e´tablir l'importance de la diversite´et des liens ge´ne´tiques entre 16 cultivars et souches ge´ne´alogiques de fraisier (FragariaÁ ananassa Duch.) cre´e´s au Canada graˆce a`des marqueurs ISSR (Inter Simple Sequence Repeat, fragments intermicrosatellitaires). Dix-sept amorces ont produit 225 bandes polymorphes ISSR-PCR. L'analyse des grappes par la me´thode des groupes-paires non ponde´re´s avec moyenne arithme´tique (UPGMA) re´ve`le une importante similitude ge´ne´tique entre les ge´notypes (de 63 % a`77 %), ce qui concorde avec les re´sultats de l'analyse en composantes principales (PCO). La re´partition ge´ographique des emplacements du programme d'ame´lioration n'explique que 1,4 % de la variation globale, ainsi que l'indique l'analyse de la variance mole´culaire (AMOVA). Les marqueurs ISSR de´tectent un degreś uffisant de polymorphisme pour qu'on puisse diffe´rencier le ge´notype des fraisiers, d'ou`l'utilite´de la technologie pour identifier les cultivars et permettre un meilleur choix des ligne´es parentales dans les programmes actuels d'hybridation du fraisier.Mots clé s: FragariaÁananassa, identification par le code ge´ne´tique, analyse a`variables multiples, hybridation, similitude ge´ne´tiqueThe cultivated strawberry (Fragaria )ananassa Duch.), an octoploid (2n 056) hybrid between the Scarlet or Virginia strawberry (F. virginiana Duch.) and the pistillate South American F. chiloensis (L.) Duch., is a dicotyledonous, perennial low-growing herb grown in most arable regions of the World. The crop is enjoyed by millions of people in all kinds of climates including temperate, Mediterranean, subtropical and tai...

Genetic Diversity, Antioxidant Activities, and Anthocyanin Contents in Lingonberry

“…ISSRs cost less and are easier to use than amplified fragmentlength polymorphisms (AFLPs) and do not require prior knowledge of flanking sequences, like SSRs (Reddy et al, 2002). ISSR markers are thought to be particularly useful for study of closely related individuals that exhibit low levels of polymorphism (Zietkiewicz et al, 1994) and have been applied as a very useful alternative to fingerprinting and genetic analysis in fruit crops including strawberry (Debnath et al, 2008), raspberry (Debnath, 2007a), clematis (Gardner and Hokanson, 2005), and plum (Liu et al, 2007).…”

<b>The genetic diversity of strawberry (<i>Fragaria ananassa</i> Duch.) hybrids based on ISSR markers</b> - doi: 10.4025/actasciagron.v35i4.16737

“…Arnau et al (2002) verified that the ISSR technique requires only a few markers to achieve the correct fingerprinting pattern of strawberry cultivars, demonstrating the potential for this technique for genetic studies in strawberries. The studies performed by Kuras et al (2004) and Debnath et al (2008), with the goals of evaluating the genetic similarity and genetic diversity, respectively, of strawberry cultivars, also confirmed the technical feasibility of ISSR markers. Furthermore, the study performed by Hussein et al (2008) developed an efficient fingerprinting pattern in six strawberry cultivars monitored with nine ISSR markers.…”

“…In addition, the explanation of less than 60% suggested the need to add more loci to more fully scatter the individuals in the dispersion using molecular data (ANDERSON; WILLIS, 2003). Debnath et al (2008) also used this approach to understand and establish the genetic relationships among strawberry cultivars in Canada. These authors obtained 8.0, 6.7, and 6.5% explanation from the first, second and third axes, respectively.…”

“…Using ISSR markers, Graham et al (1996) reported low levels of genetic diversity found in cultivars developed independently in several breeding programs across the world. Debnath et al (2008) also used ISSR markers to monitor both the level of genetic diversity between cultivars and the low selection advances in the Canadian breeding programs. In addition to the general low level of dissimilarity (0.1435) between the cultivars used as parents in our breeding program, the overall analysis, including all 84 hybrids originating from these five parents, revealed that the mean level of dissimilarity increased to 0.3937.…”

Molecular Characterization of Endangered Medicinal Plant Species Hedychium Coronarium From Eastern India

“…The genetic fidelity of vanilla using RAPD and ISSR primers [26]. More recently [27] used RAPD and ISSR markers in vanilla to assess the genetic diversity and [28] used ISSR marker to determine the level of genetic diversity and relatedness among strawberry cultivars. Previously there was no report about the molecular characterization of Hedychium coronarium from eastern India.…”