Isolation and characterization of polymorphic microsatellites in <i>Parnassius apollo</i> and <i>Euphydryas aurinia</i> (Lepidoptera)

Petenian, Frederic; Meglécz, Emese; Genson, Guénaëlle; Rasplus, Jean‐Yves; Faure, Éric

doi:10.1111/j.1471-8286.2005.00891.x

Cited by 14 publications

(16 citation statements)

References 10 publications

(13 reference statements)

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“…In particular, in other Parnassius sp. populations studied with microsatellite loci (MeglŽcz et al 1998;Keyghobadi et al 1999Keyghobadi et al , 2002Petenian et al 2005), the observed heterozygosity ranges between 0.33Ð0.68, a range into which our results fit well.…”

Section: Differences In Genetic Diversitysupporting

confidence: 75%

Parnassius apollo nevadensis: identification of recent population structure and source–sink dynamics

et al. 2017

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The final publication is available at Springer via http://dx.doi.org/10.1007/s10592-017-0931-0.eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. AbstractPopulation persistence depends in many cases on gene flow between local populations. Parnassius apollo nevadensis is an endemic subspecies of Apollo butterfly in the Sierra Nevada (southern Spain), whose populations are distributed in discrete patches at altitudes between 1850Ð2700 m. In this paper, we use 13 microsatellite loci to examine the genetic structure of this P. apollo subspecies. We revealed both a strong pattern of isolation by distance (which was stronger when calculated with realistic travel distances that accounted for topography) and sourceÐsink dynamics. The observed population genetic structure is consistent with strongly asymmetrical gene flow, leading to constant directional migration and differential connectivity among the populations. The apparently contradictory results from the clustering algorithms (Structure and Geneland) are also consistent with a recent (<100 ya) reduction in the distribution range.The results point to global warming as a possible cause of this reduction, as in other populations of this species. We identify some natural and anthropogenic barriers to gene flow that may be the cause of the recent population structure and sourceÐsink dynamics.2

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Section: Differences In Genetic Diversitysupporting

confidence: 75%

Parnassius apollo nevadensis: identification of recent population structure and source–sink dynamics

et al. 2017

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“…This study also confirmed the successful use of two established microsatellite loci for the study species, although they both showed considerable deviations from HWE across the sampled populations [16]. Such deviations in HWE, aside from being the result of null alleles, can be expected in E. aurinia and other Lepidoptera with similar life histories, for several biologically plausible reasons.…”

Section: Discussionsupporting

confidence: 78%

“…A differently labelled M13 primer can be used for each multiplex and then all multiplexes can be combined when sequencing. We also synthesized previously published primers of E. aurinia microsatellite markers [16], [34] with the addition of a M13 tail and tested these on our samples.…”

Section: Methodsmentioning

confidence: 99%

“…However, there are still a large number of studies detailing the isolation of microsatellite markers from Lepidoptera [13]–[18]. Inevitably it seems, these studies usually show a low number of loci, low levels of polymorphism, high occurrence of null alleles, or strong deviations from Hardy-Weinberg equilibrium (HWE) [12]–[14], [16], [19]. Often, it is also the case that the developed markers prove not to be transferable between geographically distant populations of the same species.…”

Section: Introductionmentioning

confidence: 99%

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Microsatellites for the Marsh Fritillary Butterfly: De Novo Transcriptome Sequencing, and a Comparison with Amplified Fragment Length Polymorphism (AFLP) Markers

et al. 2013

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BackgroundUntil recently the isolation of microsatellite markers from Lepidoptera has proved troublesome, expensive and time-consuming. Following on from a previous study of Edith's checkerspot butterfly, Euphydryas editha, we developed novel microsatellite markers for the vulnerable marsh fritillary butterfly, E. aurinia. Our goal was to optimize the process in order to reduce both time and cost relative to prevailing techniques. This was accomplished by using a combination of previously developed techniques: in silico mining of a de novo assembled transcriptome sequence, and genotyping the microsatellites found there using an economic method of fluorescently labelling primers.Principal FindingsIn total, we screened nine polymorphic microsatellite markers, two of which were previously published, and seven that were isolated de novo. These markers were able to amplify across geographically isolated populations throughout Continental Europe and the UK. Significant deviations from Hardy-Weinberg equilibrium were evident in some populations, most likely due to the presence of null alleles. However, we used an Fst outlier approach to show that these markers are likely selectively neutral. Furthermore, using a set of 128 individuals from 11 populations, we demonstrate consistency in population differentiation estimates with previously developed amplified fragment length polymorphism (AFLP) markers (r = 0.68, p<0.001).SignificanceRapid development of microsatellite markers for difficult taxa such as Lepidoptera, and concordant results with other putatively neutral molecular markers, demonstrate the potential of de novo transcriptional sequencing for future studies of population structure and gene flow that are desperately needed for declining species across fragmented landscapes.

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“…Our studies showed that the PIC of S. inferens SSRs ranged from 0.181 to 0.947; the mean was 0.633, which indicates a relatively high polymorphism. It has been difÞcult to isolate microsatellite markers for Lepidoptera species (Anthony et al 2001, Petenian et al 2005, Fauvelot et al 2006, VanÕt Hof et al 2007, Habel et al 2008, Sinama et al 2011 possibly because loci contain similar ßanking regions (Meglecz et al 2004(Meglecz et al , 2007, are associated with transposable elements (Coates et al 2008, Tay et al 2010, or contain a high frequency of null alleles (Keyghobadi et al 1999, Harper et al 2003, Meglé cz et al 2004, Zhang, 2004, VanÕt Hof et al 2007, Mikheyev et al 2010. Our result showed that 12 loci (boldface in Table 1) exhibited no signiÞcant evidence of null alleles in ampliÞed loci with high polymorphism (PIC Ͼ 0.5), which accounted for merely half of the highly polymorphic loci (PIC Ͼ 0.5; Table 1).…”

Section: Resultsmentioning

confidence: 99%

First Microsatellites from Sesamia inferens (Lepidoptera: Noctuidae)

Tang

Meng

et al. 2014

Annals of the Entomological Society of America

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The pink stem borer, Sesamia inferens (Walker) (Lepidoptera: Noctuidae), is an important pest of rice in China and other parts of Asia. The wide geographic distribution and broad host range of S. inferens is likely to result in high genetic variability within the species. To better understand the genetic structure and phylogeography of this pest, 30 polymorphic microsatellite loci were developed and characterized by fast isolation by ampliÞed fragment length polymorphism of sequences containing repeats (FIASCO). Our results from two geographic locations (Yangzhou and Guiyang) showed that the polymorphic information content (PIC) ranged from 0.181 to 0.947, with an average of 0.633 (relatively high polymorphism). The number of alleles per locus ranged from 2 to 31, with expected heterozygosities from 0.196 to 0.962 and observed heterozygosities from 0.043 to 1.000. Fourteen loci showed no signiÞcant departure from HardyÐWeinberg equilibrium after sequential BonferroniÕs correction at least in one population; furthermore, loci CA194 and GT106, GA13 and TG52, GA13 and TG51, CA31 and CA43, GT16 and GT206 showed signiÞcant linkage disequilibrium. A preliminary analysis of mutation patterns in microsatellites was undertaken, and three types of microsatellite mutation patterns were described. We conclude that the microsatellite markers described herein will be useful in studying population genetics within S. inferens. Furthermore, some of the microsatellites show high polymorphism and lack null alleles, indicating potential use in the identiÞcation of the species, S. inferens.

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Isolation and characterization of polymorphic microsatellites in Parnassius apollo and Euphydryas aurinia (Lepidoptera)

Cited by 14 publications

References 10 publications

Parnassius apollo nevadensis: identification of recent population structure and source–sink dynamics

Parnassius apollo nevadensis: identification of recent population structure and source–sink dynamics

Microsatellites for the Marsh Fritillary Butterfly: De Novo Transcriptome Sequencing, and a Comparison with Amplified Fragment Length Polymorphism (AFLP) Markers

First Microsatellites from Sesamia inferens (Lepidoptera: Noctuidae)

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