First Microsatellites from Sesamia inferens (Lepidoptera: Noctuidae)

Abstract

The pink stem borer, Sesamia inferens (Walker) (Lepidoptera: Noctuidae), is an important pest of rice in China and other parts of Asia. The wide geographic distribution and broad host range of S. inferens is likely to result in high genetic variability within the species. To better understand the genetic structure and phylogeography of this pest, 30 polymorphic microsatellite loci were developed and characterized by fast isolation by ampliÞed fragment length polymorphism of sequences containing repeats (FIASCO). Our results from two geographic locations (Yangzhou and Guiyang) showed that the polymorphic information content (PIC) ranged from 0.181 to 0.947, with an average of 0.633 (relatively high polymorphism). The number of alleles per locus ranged from 2 to 31, with expected heterozygosities from 0.196 to 0.962 and observed heterozygosities from 0.043 to 1.000. Fourteen loci showed no signiÞcant departure from HardyÐWeinberg equilibrium after sequential BonferroniÕs correction at least in one population; furthermore, loci CA194 and GT106, GA13 and TG52, GA13 and TG51, CA31 and CA43, GT16 and GT206 showed signiÞcant linkage disequilibrium. A preliminary analysis of mutation patterns in microsatellites was undertaken, and three types of microsatellite mutation patterns were described. We conclude that the microsatellite markers described herein will be useful in studying population genetics within S. inferens. Furthermore, some of the microsatellites show high polymorphism and lack null alleles, indicating potential use in the identiÞcation of the species, S. inferens.

Population genetic structure and migration patterns ofLiriomyza sativaein China: moderate subdivision and no Bridgehead effect revealed by microsatellites

“…This may arise from the Wahlund effect () related to recurrent inbreeding and subpopulation structure. It is reasonable to conclude that inbreeding has a major impact on heterozygote deficiency since sampling mainly from greenhouses restricts the randomness of mating, and since this species produces multiple generations in a year, unlike some other species with special reproductive strategies such as Aleurocanthus spiniferus (Quaintance) (Tang et al , 2014 b , 2015).…”

Towards a global DNA barcode reference library for quarantine identifications of lepidopteran stemborers, with an emphasis on sugarcane pests

“…Sesamia inferens occurs in South, South-East and East Asia, New Guinea and the Solomon Islands, and is a pest of sugarcane and several cereals 50 . Although some studies have investigated its genetic diversity within parts of this range, particularly in China 47,51 , its overall genetic diversity is not well characterised. We found the species to be paraphyletic in all of the haplotypes dataset analyses, being split into two clades (Fig.…”

Molecular phylogeography and evolutionary history of the pink rice borer (Lepidoptera: Noctuidae): Implications for refugia identification and pest management

“…For the nuclear markers, variable microsatellite loci previously identified for PRB were examined (Tang et al, 2014), and nine loci with strong, unambiguous banding patterns were selected for this study (Table S2). The PCR reactions and genotyping were conducted as described in Tang et al (2014). PCR products were analysed using an ABI 3730XL DNA sequencer.…”

“…PRB is distributed throughout paddy fields in Asian countries such as China, Japan, India and Pakistan, and was recently found in Hawaii and Guam (United States) according to Centre for Agriculture and Bioscience International (CABI) (https://www.cabi.org/isc/datasheet/49751#REF- DDB-202162). Along with the striped stem borer, Chilo suppressalis (Walker) and the brown planthopper, Nilaparvata lugens (Stål), PRB is threatening rice production and causing significant economic losses in Asia (Tang et al, 2014;Xu et al, 2011). PRB can damage almost all stages of rice plants from seedling to maturity (Lu et al, 2015;Singh, 2012).…”