Isolation and characterization of peptides from blood serum of patients with multiple sclerosis

Abstract

In order to find novel molecular markers of multiple sclerosis we developed a scheme of oligopeptides' isolation including their extraction from blood serum with 10 % trichloroacetic acid, followed by precipitation of soluble substances with acetone in ratio 6:1. Oligopeptides were dissolved in water and their characteristics was determined by gel filtration under HPLC conditions and thin layer chromatography. Obtained data have shown that blood serum of MS patients contains two oligopolypeptides with average molecular masses of 300-500 Da. We also studied biological activity of TCA-soluble peptides toward some eukaryotic and prokaryotic cells in comparison with phosphopeptides isolated from casein hydrolysates. It was found that TCA-soluble peptides are capable of effective inhibiting HeLa cells' proliferation, while their inhibitory effect was expressed toward Jurkat T-cells and was not detectable toward U373 cells. The casein's phosphopeptides were capable to stimulate proliferation of Jurkat cells and effectively inhibited growth of cells. Neither antibacterial, nor antifungal activities of these oligopeptides were detected.

Molecular-Mass Distribution of Casein Phosphopeptides

“…), у багатьох наукових публікаціях було підтверджено здатність казеїнових фосфопептидів зв'язувати іони макроелементів -кальцію, магнію, феруму, а також мікроелементівцинку, нікелю, кобальту і селену [5]. Були відкриті інші види біологічної активності для БАФП [6]. Завдяки своїм властивостям казеїнові БАФП викликають значний інтерес як функціональні інгредієнти для харчових продуктів.…”

Isolation of κ-CN-1P and Β-CN-5Р fractions from native casein micelles

“…These peptides may affect the cardiovascular (casokinins, casoplatelins), nervous (antagonists and agonists of opiate receptors), immune (immunomodulators) and digestive (regulators of motility and secretion in the gastroenteric tract, metal-binding peptides) systems. New kinds of biological action of casein peptides are discovered [4,5]. Casein belongs to heterogeneous proteins and consists of four basic (α S1 -CN, α S2 -CN, β-CN and κ-CN) and numerous minor fractions.…”