Isolation of κ-CN-1P and Β-CN-5Р fractions from native casein micelles

Yukalo, V.

doi:10.15407/ubj90.04.074

Cited by 4 publications

(3 citation statements)

References 8 publications

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“…A stable level of calcium regulates various physiological functions, including muscle contraction, release of neurotransmitters, intracellular signal transduction and blood coagulation. Calcium ions are considered one of the main intracellular second messengers, and their concentration is tightly regulated by calcium channels, ion exchangers, and pumps [23,24]. At the same time, ionized extracellular calcium acts as a primary messenger, serving as a ligand for G protein-coupled membrane calcium receptors.…”

Section: Resultsmentioning

confidence: 99%

Vitamin D(3) and methylenebisphosphonic acid in the correction of mineral metabolism disorders and bone remodeling associated with glucocorticoid-induced osteoporosis

Lisakovska¹,

Shymanskyi²,

Vasylevska³

et al. 2023

Ukr.Biochem.J.

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The study was aimed at evaluating therapeutic efficacy of vitamin D3 (VD3, 1000 IU/kg of b.w., 30 days) and sodium salt of methylenebisphosphonic acid (MBPA, 17 mg/kg of b.w., 30 days) monotherapies as well as their effect in combination in preventing mineral metabolism and bone remodeling disturbances associated with glucocorticoid(GC)-induced osteoporosis. Osteoporosis in rats was induced by long-term (30 days) administration of the synthetic glucocorticoid prednisolone (5 mg/kg of b.w.). Calcium and inorganic phosphate levels, activity of alkaline phosphatase (ALP) in serum, bone tissue and bone marrow were determined spectrophotometrically. The protein levels of VD3 receptor (VDR), receptor activator of nuclear factor kappa-B (RANK), its ligand (RANKL), and osteoprotegerin (OPG) in bone tissue were determined by Western blotting. Serum 25-hydroxyvitamin D3 (25OHD3) content was assayed by ELISA. It was shown that prednisolone caused the development of hypocalcemia and hypophosphatemia, increased the alkaline phosphatase activity in the blood serum, while downregulating its activity in bone tissue and bone marrow. GC-induced osteoporosis was accompanied by a profound deficiency of VD3 and a decrease in the content of VDR. Evaluation of the NF-κB-associated cytokine axis RANK/RANKL/OPG, which regulates the balance of osteoblasts/osteoclasts, showed a simultaneous decrease in the RANK content and OPG/RANKL ratio. Vitamin D3 restored mineral metabolism and 25OHD3 level that led to the normalization of VDR-mediated signaling and RANK/RANKL/OPG functions in bone tissue. It has been shown that the administration of MBPA had a corrective effect on the content of mineral components in the blood serum and bone tissue, as well as on the activity of alkaline phosphatase only in combination with vitamin D3, indicating a low efficiency of bisphosphonate monotherapy in GC-induced vitamin D3 deficiency and osteoporosis. Keywords: bone remodeling, glucocorticoid-induced osteoporosis, methylenebisphosphonic acid, RANK/RANKL/OPG axis, vitamin D3

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Section: Resultsmentioning

confidence: 99%

Vitamin D(3) and methylenebisphosphonic acid in the correction of mineral metabolism disorders and bone remodeling associated with glucocorticoid-induced osteoporosis

Lisakovska¹,

Shymanskyi²,

Vasylevska³

et al. 2023

Ukr.Biochem.J.

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“…Milk and milk products are considered the primary sources of nutrients in the human ration, offering quality proteins, minerals, vitamins, and energy [3]. In addition, milk is an excellent matrix for the release of bioactive compounds [4], and various dairy products are the basis for enrichment with other nutrients [5], [6]. Currently, the development of new formulations of dairy products that consumers would like very much is one of the main forces in the dairy industry.…”

Section: Introductionmentioning

confidence: 99%

Microbiological characteristics of hard cheese with flax seeds

Kukhtyn,

Arutiunian,

Pokotylo

et al. 2024

Potr. S. J. F. Sci.

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Highly nutritious dairy products such as hard cheeses are considered a good source of protein, fats, mineral substances, and vitamins and are consumed in significant quantities. At the same time, the disadvantages of cheeses include the presence of a large amount of saturated fatty acids in their composition, which are associated with the development of cardiovascular diseases. Therefore, modifying the composition of fatty acids in hard cheese by increasing the content of unsaturated fatty acids and reducing the amount of saturated fatty acids is extremely important for consumers' health. This research aimed to determine the dynamics of microbiological indicators in the ripening technology of hard rennet cheese with different contents of flax seeds as a source of omega-3 fatty acids. The technology of hard rennet cheese with 3-5% flax seed content was investigated. A 1.3 times higher content of lactic acid microflora was found in samples of hard cheese with 5% flax seeds during the first 10 days of ripening compared to the control sample of cheese. On the 60th day of ripening of cheese with a content of 5% flax seeds, the number of lactic acid bacteria was 9.4 ±0.3×109 CFU/g, and in the control cheese – 7.8 ±0.3×109 CFU/g. During the production and ripening of cheese with different content of flax seeds, no exceedance of normative values was found for the number of Enterobacteriaceae and Staphylococcus aureus bacteria. A method (washing the seeds in a sodium bicarbonate solution and drying at a temperature of 95 ±1 for 20 min) of processing flax seeds before adding them to the cheese mass was proposed, which reduced the number of mesophilic microorganisms by approximately 200 times to 3.9 ±0.1×101 CFU/ g, fungi by 160 times to 0.3 ±0.1×101 CFU/g and aerobic mesophilic bacilli by 78 times to 1.1 ±0.1×101 CFU/g. Therefore, the developed hard rennet cheese with flax seeds can be consumed as an additional source of omega-3 fatty acids and dietary fiber.

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“…Among all LAB metabolites, bacteriocins deserve special attention due to their high potential for inhibiting activity towards pathogenic bacteria (Wang & Liu, 2016;Ahn et al, 2017;Zhang et al, 2018). Bacteriocins are lowmolecular peptides or genetically-coded proteins which are synthesized on ribosomes and produced extracellularly (Cavicchioli et al, 2017;Yukalo & Storozh, 2018). After binding with the surficial receptors or penetrating into target cells, bacteriocins can function in them through formation of pores, degradation of cellular DNA and inhibition of the system of pepti-doglycan synthesis.…”

Section: Introductionmentioning

confidence: 99%

Antagonistic activity of strains of lactic acid bacteria isolated from Carpathian cheese

Musiy

Tsisaryk

Slyvka

et al. 2020

Regul. Mech. Biosyst.

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A promising area for improving probiotics is the search for new sources of strains and the development of complex preparations which would include different types of bacterial cultures that complement each other. Sources of selection may be traditional dairy products, in particular, cheeses made from raw milk. Wild strains can be endowed with antibacterial properties. The antagonistic action of lactic acid bacteria (LAB) has long attracted the attention of researchers and scientists. The aim of the study was to investigate the antagonistic activity against pathogenic and opportunistic microorganisms of LAB strains isolated from traditional Carpathian cheese. Three samples of cheese were selected for the research – one sample of brynza and budz (brynza before salting), made in the highlands of the Carpathians, and one sample of budz, made in the foothills. LAB were identified using classic microbiological and modern molecular genetic methods (RAPD-PCR, RFLP-PCR, 16S rRNA gene sequencing). The objects of our studies were five strains of LAB: Lactococcus lactis IMAU32258, L. garvieae JB2826472, Enterococcus durans FMA8, E. faecium L3-23, E. faecium IMAU9421. Technological parameters such as acid-forming activity of milk fermentation, resistance to high concentrations of NaCl and temperature optimums of cultivation were taken as the main criteria for assessing the suitability of LAB for inclusion in fermentation preparations. Antagonistic activity was determined by agar diffusion (agar well method) and optic density of test cultures using a Multiscan FC microplate reader (Thermo scientifiс, USA) at the wave of 620 nm. There were four reference strains of pathogenic and opportunistic microorganisms were test cultures: Listeria monocytogenes PCM 2191, Staphylococcus aureus PCM 458, Escherichia coli PCM 2208, Salmonella typhimurium PCM 2182. Strains of the test cultures were received from the collection of microorganisms of the Institute of Biology and Biotechnology the (University of Rzeszów, Poland). According to the ability of LAB strains to form lactic acid, L. lactis IMAU32258 was the best acid-forming agent with an acid-forming energy of 94 °T. E. faecium was characterized by moderate levels of active and titratable acidity. Less pronounced acid-forming ability was determined for the species E. durans and L. garvieae. Cultures of the genus E. faecium, L. garvieae and E. durans were the most resistant to high concentrations of NaCl (6.5%). Regarding temperature optimums, we found that strains of E. faecium and E. durans species grew both at temperatures of 10, 15 and 45 °C, whereas no growth of L. lactis IMAU32258 and L. garvieae JB282647 2 was observed at 45 °C. Among the studied bacteria, the strains of E. durans FMA8 and E. faecium L3-23 were characterized by the highest antagonistic activity in producing the largest zones of growth inhibition and optic density of pathogenic and opportunistic microorganisms. The strain L. garvieae JB282647 2 exhibited the lowest level of antagonistic activity against pathogenic and opportunistic microorganisms.

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Isolation of κ-CN-1P and Β-CN-5Р fractions from native casein micelles

Cited by 4 publications

References 8 publications

Vitamin D(3) and methylenebisphosphonic acid in the correction of mineral metabolism disorders and bone remodeling associated with glucocorticoid-induced osteoporosis

Vitamin D(3) and methylenebisphosphonic acid in the correction of mineral metabolism disorders and bone remodeling associated with glucocorticoid-induced osteoporosis

Microbiological characteristics of hard cheese with flax seeds

Antagonistic activity of strains of lactic acid bacteria isolated from Carpathian cheese

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