Isolation and Characterization of Pathogens Responsible for Urinary Tract Infection in Bangladesh and Determination of their Antibiotic Susceptibility Pattern.

Abstract

The aim and objectives of this study were to determine the etiological pathogens of the Urinary Tract Infection and to determine their antibiotic sensitivity pattern in Bangladesh. This study was carried out on clinically suspected UTI patient in a Private Diagnostic Centre, Bangladesh from May 2015 to November 2015. From total 200 urine samples, pathogens were isolated and identified and their antibiotic susceptibility was observed by standard microbiological procedures. Out of 200 urine samples 124 (62%) patients tested positive for culture. In gram negative bacteria, the most predominant was the E. coli (48.39%) followed by Klebsiella spp (14.52%), Pseudomonas spp (3.23%) and Acinetobacter spp (3.23%). In the gram positive bacteria Staphylococcus aureus (13.71%) and Enterococcus spp (11.29%) were found. Occasionally Candida spp. (5.65%) was found. Gramnegative bacteria were more sensitive to Imipenem, Amikacin and Nitrofurantoin. On the otherhand Grampositive bacteria showed their sensitivity to Amoxicillin-clavulinic acid and Linezolid and Vancomycin showed 100% sensitivity. UTI has become difficult to treat due to appearance of pathogens with increasing resistance to antimicrobial agents. This study showed that pathogens responsible for UTI showed increasing resistance to the commonly prescribed drugs that in turn leaves very few alternative options for the treatment of UTIs.

Serological and Molecular detection of Staphylococcus aureus isolated from UTI patients

“…aureus. Outside Iraq, the present results agreed with results of study [19] who document that (13.71%), of isolates identified as Staph. aureus that isolated from UTI patients.…”

Isolation of Uropathogens and Their Antibiotic Susceptibility Pattern at a Tertiary Care Hospital in Northern India

“…Common signs and symptoms include fever, dysuria, increased frequency of micturition, chills, and cloudy or foul-smelling urine [4]. Almost 95% of all UTIs are caused by bacteria which include Gram-negative bacteria (80-85%) and Gram-positive bacteria (15-20%) [1,5].…”

In-vitro study of the activity of some medicinal plant leaf extracts on urinary tract infection causing bacterial pathogens isolated from indigenous people of Bolangir district, Odisha, India

“…The results obtained from the microscopic study indicated the prevalence of both Gram positive as well as Gram negative bacterial strains, with coccus and bacillus forms, in the tested urine samples isolated from patients suffering from UTIs. Mollick et al [16] and Sewify et al [17] also reported the abundance of both Gram positive and Gram negative bacterial pathogens (with coccus and bacillus forms) in patients with urinary tract infection. This indicated that the Gram positive as well as Gram negative bacteria are equally responsible for UTI and the immune system might not be discriminating and preventing either of them from the infection process.…”