Serological and Molecular detection of Staphylococcus aureus isolated from UTI patients

Abstract

Urinary tract infections (UTIs) are one of the commonest infections encountered by clinicians despite the widespread availability of antimicrobial agents. Such Infections caused by different bacterial pathogens that can be acquired through both hospitals and the community. The present study is aimed to isolation and diagnosis of Staph. aureus , detecting the optimal antimicrobial for the treatment of these infections. A total of (600) mid-stream urine samples were collected during the period from August to December, 2018 from patients who were complained from UTIs at AL-Hussain Teaching Hospital in AL-Nasiriyah City, Southern Iraq. The study included isolation and diagnosis of Staph. aureus based on morphological, microscopic characterization, biochemical tests and confirmed by API-20 and Vitek2 systems. In addition , all Staph. aureus isolates were subjected to the serological diagnosis of protein A by using a latex agglutination test and convention PCR technique was used to detect the presence of the 16S rRNA gene (353bp). 380 (63.33%) were positive isolates for bacteriological examination, the Staph. aureus was identified with 50 samples (13.5 %). In addition, all Staph. aureus isolates were assayed for antimicrobial susceptibility against 14 selected antibiotic discs by using the disc diffusion method. All isolates were completely resistant to Penicillin (P), Oxacillin (Ox), and Ampicillin (Amp). While the most effective antibiotics were Nitrofurantoin, Gentamycin, isolates were susceptible to these antibiotics in 76%, and 60%, respectively. Also, In the analysis of the nucleotides sequence of the partial 16S rRNA gene, the results were showed that these bacteria actually related to Staph. aureus ,according to current result, three of these isolates registered globally in the NCBI Gen bank, and the accession numbers of these isolates are (MK910079.1),( MK910080.1), and (MK910081.1).

Serological diagnosis of IL-1β and IL-6 associated with Urinary tract infections among pregnant women in Thi-Qar Governorate

“…The current study's findings showed that IL-6 was found in every serum sample, with a concentration range of 0.158-89.425 pg/ml. Also, patients with UTIs had lower mean serum levels of IL-1β (6.91 ± 11.78 pg/ml) than the control group (43.32 ± 34.62 pg/ml), as table (1) illustrates. These results were with significant differences between the patient and control groups (p≤ 0.001).…”

“…As shown in Table (1), pregnant women with UTIs had lower mean serum levels of IL-6 (12.08 ±16.35 pg/ml) than in the control group (42.19 ± 28.0 pg/ml). This outcome was with high significant differences (p≤ 0.002).…”

“…UTIs are classified as lower urinary tract infections if they affect the bladder or urethra, and upper UTIs if they affect the kidneys and ureter. There are two categories of UTIs, simple and complex [1]. Because of their shorter urethra, which is constantly polluted with infections from the vagina and rectum, women are more vulnerable than men.…”