Isolation and characterization of Ornithobacterium rhinotracheale from chickens in Brazil

Canal, Cláudio Wageck; Leão, Joice Aparecida; Rocha, Silvio L.S.; Macagnan, Marisa; Lima-Rosa, C. A. V.; Oliveira, Sı́lvia Dias de; Back, Alberto

doi:10.1016/j.rvsc.2004.10.003

Cited by 33 publications

(26 citation statements)

References 17 publications

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“…All of the O. rhinotracheale isolates gave a clear band at 784 bp by using the PCR with O. rhinotracheale-specific primers under the recommended conditions, but the primer pair also amplified fragments of Riemerella anatipestifer RA37 and RA38 (500 bp), Riemerella columbina RC29 (1,200 bp), and Coenonia anatina CA39 (200 bp). That finding is in agreement with a previous study that noted that false-positive results were recorded using this set of primers (41). Therefore, it is recommended that the identification of O. rhinotracheale be confirmed by sequencing the amplified fragment.…”

Section: Discussionsupporting

confidence: 92%

“…With respect to the biochemical profiles, tested O. rhinotracheale isolates (SCV and WT isolates) showed poor positive rates using API-20NE identification strips, in agreement with previous studies that reported low positive rates for biochemical reactions (15,41). Two codes were obtained for O. rhinotracheale WT isolates (codes 0020004 and 0220004), in accordance with previous studies that reported that these two codes are prevalent and are found for about 99.5% of O. rhinotracheale isolates (17,20,21).…”

Section: Discussionsupporting

confidence: 90%

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Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

et al. 2013

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bOrnithobacterium rhinotracheale is a Gram-negative bacterium associated with respiratory diseases in many avian species, with worldwide distribution, and it causes significant economic loss to the poultry industry. In this study, the isolation and characterization of O. rhinotracheale small-colony variants (SCVs) are described for the first time. O. rhinotracheale isolates (n ‫؍‬ 27) were recovered from tracheal samples (n ‫؍‬ 321) collected from different avian species with clinical signs of respiratory disease. Of the 27 O. rhinotracheale isolates, 21 (77.8%) showed SCVs in their primary cultures. Five O. rhinotracheale SCV isolates showed high levels of stability and were chosen for further characterization with their wildtype (WT) isolates. Stable O. rhinotracheale SCVs were oxidase negative, while their WT isolates were positive. Growth curves for stable O. rhinotracheale SCVs indicated lower growth rates and longer lag phases than for their WT isolates. Furthermore, it was possible to increase the efficacy of the broth medium in supporting the growth of O. rhinotracheale WT isolates by supplementing it with 5% fetal bovine serum (FBS) and 2% IsoVitaleX Enrichment. Antibiotic susceptibility tests showed that O. rhinotracheale SCVs had higher MIC values than their WT isolates. This study suggests that successful antibiotic treatment of respiratory diseases associated with O. rhinotracheale must take into consideration the resistance patterns of O. rhinotracheale SCVs. Intracellular persistence in murine RAW 264.7 macrophages revealed that O. rhinotracheale SCV28 had higher survival rates than its WT isolate. Finally, small-colony variants may be important contributors to the pathogenesis of O. rhinotracheale.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

et al. 2013

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“…Concurrent infection of young poultry with Klebsiella pneumoniae increased the severity of respiratory disease . Weakness, gasping, pump-handled respiration, dyspnoea, mucous discharge and mortality, swelling of sinuses, facial oedema, tracheitis, exudative pneumonia, pleuritis, air sacculitis, pericarditis, sinusitis, drop in egg production and poor egg quality characterize the respiratory infection (Zorman et al, 2000;Canal et al, 2005 Confirmatory diagnosis of bacterial diseases of upper respiratory tract infection in poultry requires more data. The investigation was designed to (a) isolate and identify the bacteria from nasal sinuses and trachea and to (b) determine the pathological lesions caused by these bacteria.…”

Section: Introductionmentioning

confidence: 99%

Pathological study on the upper respiratory tract infection of chickens and isolation, identification of causal bacteria

et al. 2012

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The proportional occurrence of bacteria and pathological lesions in the nasal sinuses and trachea of dead chickens were determined during [2008][2009]. Nasal sinus and tracheal swabs from 50 dead birds were collected in sterile nutrient broth. The histopathological samples were collected in 10% neutral buffered formalin and studied with light microscope. The isolation and identification of bacteria were performed by culture, staining and biochemical tests. The proportional occurrence of bacteria in trachea (n = 50) and nasal sinuses (n = 50) of dead chickens was Klebsiella sp.

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“…van Empel and Hafez (1999) and Chin and Charlton (2008) also proposed the use of blood agar plates without antibiotic to prevent missing 10% of the antibiotic-susceptible isolates. Ornithobacterium rhinotracheale was first identified as a non-hemolytic microorganism (van Empel and Hafez, 1999;Hafez, 2002;Canal et al, 2005;Chin and Charlton, 2008;, such as the ATCC 51463 strain of O. rhinotracheale (Tabatabai et al, 2010;Gornatti Churria et al, 2011). However, the presence of extensive and unusual β-hemolytic activity has been recently reported among North American and Argentinean field isolates after the 48-h-period following incubation at room temperature (Walters et al, 2009;Tabatabai et al, 2010;Gornatti Churria et al, 2011).…”

Section: Etiologymentioning

confidence: 99%

“…Although O. rhinotracheale is considered as a primary pathogen in poultry (van Veen et al, 2000b;Pan et al, 2012), the severity of clinical signs, duration of the disease, and mortality are extremely variable and are influenced by housing environmental stressors such as poor management, inadequate ventilation and high ammonia levels, high stocking density, poor litter conditions, poor hygiene, foodborne mycotoxins, suboptimal nutrition, and concomitant infectious diseases Hoerr, 2010). After its identification and characterization in 1994 by Charlton et al, O. rhinotracheale has been isolated throughout the world Devriese et al, 1995;van Empel et al, 1997;Goovaerts et al, 1998;Sakai et al, 2000;Sprenger et al, 2000;van Veen et al, 2000a;Ak and Turan, 2001;Devriese et al, 2001;Hung and Alvarado, 2001;Soriano et al, 2002;Malik et al, 2003;Banani et al, 2004;Canal et al, 2005;Türkyilmaz, 2005;Tsai and Huang, 2006;Marien, 2007;Chansiripornchai et al, 2007;Moreno et al, 2008;Murthy et al, 2008b;Uriarte et al, 2009;Walters et al, 2009;Gavrilović et al, 2010;Tabatabai et al, 2010;Chernyshev et al, 2011;Gornatti Churria et al, 2011;.…”

Section: Introductionmentioning

confidence: 99%

<i>Ornithobacterium rhinotracheale</i> Infection in Poultry: an Updated Review

Churria¹,

Machuca²,

Vigo³

et al. 2012

ijmz

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Ornithobacterium rhinotracheale is a Gram-negative bacterium of the rRNA superfamily V within the CytophagaFlavobacterium-Bacteroides phylum, which has become an emerging pathogen in the poultry industry. The purpose of this work is to review the current literature on O. rhinotracheale infection in domestic poultry. Despite the difficulties to identify the bacteria using biochemical tests, the polymerase chain reaction (PCR) technique has been successfully used to identify suspected isolates. Moreover, pulsed-field gel electrophoresis (PFGE) has allowed the genetic typing of O. rhinotracheale. Recent investigations carried out in the United States and Argentina described the unusual and extensive β-hemolytic activity of field strains isolated from affected chickens and turkeys. According to the latest serological studies, the causing agent has eighteen serotypes (A to R) and serotype A is the most prevalent among chicken and turkey strains. Gross findings typically found in broiler chickens are unilateral pneumonia, pleuritis, and abdominal airsacculitis with foamy, white yogurt-like exudate, which cause increased condemnation rates at slaughter, whereas those found in turkeys include tracheitis, pneumonia or bronchopneumonia, thoracic and/or abdominal airsacculitis, pericarditis, and peritonitis. Despite the worldwide efforts to control O. rhinotracheale infection, the present situation shows several problems not only because antibiotic treatments appear to be less effective due to the resistance acquired in the poultry industry but also because the vaccines developed so far show variable results in commercial poultry.

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Isolation and characterization of Ornithobacterium rhinotracheale from chickens in Brazil

Cited by 33 publications

References 17 publications

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

Pathological study on the upper respiratory tract infection of chickens and isolation, identification of causal bacteria

<i>Ornithobacterium rhinotracheale</i> Infection in Poultry: an Updated Review

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