Pathological study on the upper respiratory tract infection of chickens and isolation, identification of causal bacteria

Popy, N; Asaduzzaman, M.; Miah, Mohammad Alam; Siddika, Ayesha; Sufian, M. A.; Hossain, MM

doi:10.3329/bvet.v28i2.10677

Cited by 10 publications

(10 citation statements)

References 11 publications

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“…7) was 8.69% ( 2 Klebsiella sp. in 23 isolates from 30 nasal passage swabs) in the present study which was higher than the values reported by Popy et al (2011) in Bangladesh. In that study the prevalence of Klebsiella sp.…”

Section: Colony Characters Staining Characters and Biochemical Testscontrasting

confidence: 56%

“…Recent investigation (Popy et al, 2011) in Bangladesh showed that the prevalence of bacteria 6.03% Klebsiella sp., 38.79% Escherichia coli., 8.26% Pasteurella sp., 5.17% Bacillus sp., and 41.37% Staphylococcus sp., were present in both trachea (n=50) and nasal sinuses (n=50) of dead chickens.Thus, it is revealed that the bacteria of nasal passages of chickens cause a great loss to the farmers. So, the present investigation has been undertaken to enrich the knowledge of stockholders with a view (a) to isolate, identify and detect the prevalence of the bacteria from nasal passage of chickens and (b) to determine the pathological lesions in nasal passage.…”

Section: Ornithobacterium Rhinotracheale Has Recentlymentioning

confidence: 99%

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Bacteriological and Pathological Investigation of Nasal Passage Infections of Chickens (Gallus gallus)

et al. 2013

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An experiment was conducted at the Bangladesh Agricultural University, Mymensingh during July 2011 to May 2012 to investigate the prevalence of bacteria as well as pathological lesions in the nasal passages of dead chickens. Thirty nasal passage swabs from 30 dead birds (20 from SK Veterinary Diagnostic Centre (SKVDC) and 10 from the Department of Pathology, BAU were collected in sterile nutrient broth. The histopathological samples (n=6) were collected in 10% neutral buffered formalin. The isolation and identification of bacteria were performed by culturing in different media, staining and biochemical tests. The collected tissues were fixed, processed, sectioned, stained and studied with light microscope. The prevalence of bacteria 30.43% Escherichia coli, 47.83% Staphylococcus sp., 13.04% Pasteurella sp., and 8.69% Klebsiella sp. were found in nasal passages (n=30) of dead chickens. Two cases of mixed infection with E. coli and Pasteurella isolates, and one case with Klebsiella and E. coli isolates were identified. Six tissue samples of grossly identifiable lesions such as congested and mucus filled nasal passages from 6 dead chickens were processed for histopathology. Microscopically, the section of nasal passages in general showed congested mucosa with excessive infiltration of heterophils and lymphocytes. The lining epithelia of nasal passage revealed acanthosis, hyperkeratosis and disruption of nasal epithelia. There was also hyperplasia of different mucous glands of nasal passages. Klebsiella sp. affected nasal tissues showed comparatively severe lesions than that of other bacterial infection in chickens.

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Section: Colony Characters Staining Characters and Biochemical Testscontrasting

confidence: 56%

Section: Ornithobacterium Rhinotracheale Has Recentlymentioning

confidence: 99%

Bacteriological and Pathological Investigation of Nasal Passage Infections of Chickens (Gallus gallus)

et al. 2013

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“…(10.2%),Dashe et al (2013); (8.8%)Dashe et al(2008), N. Popy et al (2011, and are differed with the result received by Burtram C. Fielding et al(2010),Rajaa, et al (2011) who recorded higher isolation rate of Klebsiella pneumoniae (40.4), Botchris et al(2012): klebsiella isolated associated with bile and intestinal content of slaughtered chickens (63%) of samples. Also lower prevalence rate was recorded byHajieh (2008) resuts who isolated klebsiella form (1%) only of samples tested,Dashe et al (2008) detected klebsiella in (1.5%) of samples(Aher et al 2012) isolated klebsiella in (6.5%) of collected samples.…”

mentioning

confidence: 82%

Isolation and Molecular Iidentification of Klebsiella Microbe Isolated from Chicks

Aly¹,

Khalil²,

Metwaly³

2014

AJVS

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Klebsiella pneumoniae is one of the most common infectious disease affecting chicks, causing great economic losses. Also, possess food safety and antimicrobial resistance threats, as it may act as a contamination source of poultry meat and eggs. We aim to isolation and identification of klebsiella organisms causing death and loss in chicks. To perform this aim, it is necessary to full following points: Isolation of klebsiella causing respiratory manifestations and death in chicks purification and identification (morphologically, culturally, biochemically) of recovered klebsiella, antibiotic sensitivity test, PCR detection of Klebsiella genus specific gene (GyrA), PCR detection of klebsiella carbapenem resistance gene (kpc) and PCR detection of two of important virulence genes ; Mucoviscocity attached gene (MagA) and Iron uptake system gene (Kfu). A total of 150 chicks from different locations were examined clinically for respiratory symptoms lung, liver , trachea , intestine and samples (dropping and oro-pharyngeal swab) were collected for bacteriological examination. The results of biochemical tests for detection of biochemical characteristics of isolated Klebsiella; where all the examined isolates cleared that Klebsiella, gave positive reaction for catalase test, voges proskeure, urease and citrate test, meanwhile the isolates were negative for oxidase, Indole and Methyl red tests. Cultivation of isolate on macConkey gives lactose fermenting colonies, more or less dome shaped,3-4 mm diameter after overnight incubation at 37C .The sixteen klebsiella isolates subjected for PCR detection and the results for detection of Klebsiella genus specific gene (GyrA) were positive for all isolates. Also ten of sixteen subjected to PCR for detection of Klebsiella pneumonia carbapnamase gene (KPC) and the results were negative , ten of sixteen subjected to PCR for detection of Mucoviscocity attached gene (Mag A)and the results were positive for seven out of ten isolates,eight out of sixteen isolates subjected to PCR for detection of Iron uptake system gene (Kfu) and the results were positive for eight out of ten isolates. The 16 Klebsiella isolates were tested for the resistance to antibiotics and the results indicates that Meropenem, Impienem, Amikacin , Cefotaxime sodium, Ceftazidime , Ciproflaxin and Tobramycin had high effect on klebsiella isolates. Meanwhile, Gentamycin and Cefepime have moderate effect on Klebsiella. Moreover, antibiotics have less effect on the isolated Klebsiella were Azithromycin and Erythromycin.

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“…Under field conditions, staphylococcus sp. are commonly recovered from chicken’s respiratory tract tissues with respiratory clinical symptoms, with a higher prevalence among other causative agents (50.2% as reported by Türkyilmaz [ 60 ] and 41.4% as reported by Popy, et al [ 61 ]).…”

Section: Avian Influenza and Other Bacterial Co-infectionsmentioning

confidence: 99%

Avian Respiratory Coinfection and Impact on Avian Influenza Pathogenicity in Domestic Poultry: Field and Experimental Findings

Samy¹,

Naguib

2018

Veterinary Sciences

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The avian respiratory system hosts a wide range of commensal and potential pathogenic bacteria and/or viruses that interact with each other. Such interactions could be either synergistic or antagonistic, which subsequently determines the severity of the disease complex. The intensive rearing methods of poultry are responsible for the marked increase in avian respiratory diseases worldwide. The interaction between avian influenza with other pathogens can guarantee the continuous existence of other avian pathogens, which represents a global concern. A better understanding of the impact of the interaction between avian influenza virus and other avian respiratory pathogens provides a better insight into the respiratory disease complex in poultry and can lead to improved intervention strategies aimed at controlling virus spread.

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Pathological study on the upper respiratory tract infection of chickens and isolation, identification of causal bacteria

Cited by 10 publications

References 11 publications

Bacteriological and Pathological Investigation of Nasal Passage Infections of Chickens (Gallus gallus)

Bacteriological and Pathological Investigation of Nasal Passage Infections of Chickens (Gallus gallus)

Isolation and Molecular Iidentification of Klebsiella Microbe Isolated from Chicks

Avian Respiratory Coinfection and Impact on Avian Influenza Pathogenicity in Domestic Poultry: Field and Experimental Findings

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