Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)

Abstract

Twenty-four microsatellite DNA markers were isolated and characterized for golden cuttlefish (Sepia esculenta) from a (GT)13—enriched genomic library. Loci were tested in 48 individuals from Jiaozhou bay of China. The numbers of alleles per locus ranged from two to 25 with an average of 10.3. The observed and expected heterozygosities ranged from 0.063 to 0.896 and from 0.137 to 0.953, with averages of 0.519 and 0.633, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction and no significant linkage disequilibrium between loci pairs was detected. These microsatellite markers would be useful for analyzing the population genetic structure to make conservation and management decisions for S. esculenta.

Development and characterization of microsatellite markers for Chaeturichthys stigmatias (Actinopterygii: Gobiiformes: Gobiidae) based on restriction site-associated DNA sequencing (RAD-seq)

“…Exploring genetic diversity and population genetic structure may lead to a better understanding of the ecological importance of Gobiidae (see Yuan et al 2012;Meng et al 2017). Among molecular markers, microsatellites, or simple sequence repeats (SSR), is a simple repeat that is uniformly distributed in eukaryotic genomes and consists of tandem repeats of 2-6 nucleotides (Edwards et al 1991).…”

Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho

“…The microsatellite markers are highly polymorphic and the data is relatively easy to statistics, which is proven to be an extremely valuable tool for genetic studies and conservation and management of genetic resources [7–10]. …”

“…Species identification and population genetics assessment may lead to a better understanding of the effects of over-exploitation and environmental change on the fishery stocks. The microsatellite markers are highly polymorphic and the data is relatively easy to statistics, which is proven to be an extremely valuable tool for genetic studies and conservation and management of genetic resources [ 7 – 10 ].…”