Development and characterization of microsatellite markers for Chaeturichthys stigmatias (Actinopterygii: Gobiiformes: Gobiidae) based on restriction site-associated DNA sequencing (RAD-seq)
Abstract:Chaeturichthys stigmatias Richardson, 1844, a fish species of the family Gobiidae, is an offshore warm-temperate fish species and a dominant component of estuarine ecosystems. In this study, restriction site-associated DNA sequencing was adopted to analyze the traits of candidate microsatellite markers for C. stigmatias, and 30 polymorphic loci were developed. A total of 5631 microsatellites with primer fragments were detected, among which trinucleotide repeats (57.56% of the total simple sequence repeats) wer… Show more
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