Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho

Abstract

Twenty microsatellite markers were isolated and characterized for Solenocera crassicornis from a (GT)13-enriched genomic library. Their polymorphisms were investigated using 44 wild individuals from the South Yellow Sea. Our investigation revealed that all the markers were polymorphic. The number of alleles per locus varied from 6 to 19 with an average of 12.35. The observed and expected heterozygosities ranged from 0.400 to 0.977 and from 0.609 to 0.940, with averages of 0.788 and 0.859, respectively. Four loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction. Cross-species amplification was also conducted in Solenocera melantho collected from the East China Sea. The result showed that 14 loci could be amplified from Solenocera melantho DNAs. These polymorphic markers would be useful for assessment of genetic variation and population structure of S. crassicornis and S. melantho.

Búsqueda de primers y optimización de técnicas para la determinación de marcadores de ADN microsatélites en langostino argentino (Pleoticus muelleri)

“…Se seleccionaron cuatro (4) iniciadores (ZG-29, ZG-66, ZG-71 y ZG-85; ver Tabla 1) que permitieron amplificar microsatélites en otras especies de la misma familia (Solenoceridae) y cuyos accesos en GenBank son JQ954752, JQ954738, JQ954740 y JQ954742 (Liu et al 2012). La reacción en cadena de la polimerasa se llevó a cabo en un termociclador MultiGene™ OptiMax (LabNet).…”