PCR-based assessment of shellfish traceability and sustainability in international Mediterranean seafood markets

Galal-Khallaf, Asmaa; Ardura, Alba; Borrell, Yaisel J.; García‐Vázquez, Eva

doi:10.1016/j.foodchem.2016.01.131

Cited by 24 publications

(9 citation statements)

References 26 publications

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“…Information on the details of commercial practices and providers is unfortunately currently unavailable. These percentages are slightly higher than those previously published in other recently conducted cephalopod studies . This result, even if not completely representative of the global cephalopod market (due to the limitations in the sampling), raises serious concerns and prompts wider traceability studies by the responsible regulatory agencies.…”

Section: Discussioncontrasting

confidence: 60%

“…DNA testing of cephalopod food products and ingredients is an effective strategy to identify and authenticate species, detect contaminants or adulterants, and verify label claims. This strategy can also support traceability regulations and prevent food fraud and health problems . Previous studies have reported over 20% of ambiguity and attempts to mislead consumers by using incorrect labels when buying products labeled with the commercial name Galician octopus, which is used to legally protect this high value‐added product in national and international markets .…”

Section: Introductionmentioning

confidence: 99%

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Lab experience with seafood control at the undergraduate level: Cephalopods as a case study

Fernández

Rodrı́guez-Muñiz

Molina

et al. 2020

Biochem Molecular Bio Educ

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The correct labeling of seafood is important to protect nature and the rights of consumers. Given the certainty that the resources of the sea are not inexhaustible, only strict regulations and the implementation of sustainable fishing systems and reliable and traceable marketing systems can help ensure the long-term sustainability of fishery resources. Detecting mislabeling and seafood fraud is a useful resource for improving students' motivation and developing active learning methodologies in higher education. In the present study, we have proposed to the students a lab exercise consisting of exploring 25 different commercial cephalopod products from three major European supermarkets by using DNA barcoding and analyzing the results under the framework of EU and Spanish regulations. The problem is connected with the last theme (traceability) of the Conservation Genetics and Breeding course with the aim of providing students with a practical research lab experience about a real problem before going deeper into more theoretical contents. In this way, they can use the knowledge and the skills they acquired previously to better comprehend and think critically about the problem.Findings from students' answers to a survey revealed that the use of this approach generates useful information for communities, increases curiosity and feelings of benefit, and leads to high levels of satisfaction with lab practices compared with those in other courses. In conclusion, lab exercises focused on seafood control, in addition to being viable, can be used as a tool in classes to improve students' commitment to higher education. K E Y W O R D Sbarcoding, cephalopods, lab experiences, seafood control, university

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Section: Discussioncontrasting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Lab experience with seafood control at the undergraduate level: Cephalopods as a case study

Fernández

Rodrı́guez-Muñiz

Molina

et al. 2020

Biochem Molecular Bio Educ

Self Cite

View full text Add to dashboard Cite

show abstract

“…Similarly, a minimum detection limit of 0.1-0.01% (w/w) for meat products was found in various literatures (Karabasanavar et al, 2013;Ali et al, 2014;Amaral et al, 2015;. Some other workers, especially by real-time PCR and other PCR methods, could even detected <0.01% (w/w) in the adulterated meat mixtures (Kesmen et al, 2012;Cho et al, 2014;Floren et al, 2015). However, the second approach is most preferred since it is closer to the actual conditions of meat adulteration in the market.…”

Section: Sensitivity Testmentioning

confidence: 85%

Molecular detection of adulteration in commercial buffalo meat products by multiplex PCR assay

2019

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Authentication of commercial buffalo meat products has become a market concern. This study intended to develop and validate a highly species-specific multiplex PCR assay for authentication of buffalo meat products. Four pairs of species-specific primers were used to target mitochondrial cytochrome oxidase O (CDO) gene. The assay generated the expected PCR products of 313, 255, 294 and 177 bp for buffalo meat, cattle meat, pork meat and duck meat, respectively. The multiplex PCR assay was sensitive enough to detect 1 pg pure DNA and 0.1% (w/w) adulterated meat under mixed matrices. Market survey revealed about 35.3% of buffalo meat products are adulterated with cattle meat, pork meat or duck meat in China. The adulteration was found in all food product types including minced meat, frozen rolls, boiled meat, meat ball, vacuum-packed meat and jerky. These findings showed that multiplex PCR assay are potentially reliable techniques for detection of adulteration in raw and processed buffalo meat products.

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“…Mislabeling of food products has been mentioned as a major public health risk associated with food safety in the food market in 17 of the full-text articles included in this review [68][69][70][71][72][73][74][75][76][77][78][79][80][81][82]154]. Mislabeling of food products includes false advertising, deliberately or accidentally leaving out ingredients, not listing potential health effects, and claiming a food contains ingredients that it does not for financial gain with the intent of deceiving the consumer regarding what is actually in the package [155].…”

Section: Sood M 2014 [67] Indonesia Field Inspectionmentioning

confidence: 99%

Public health risks related to food safety issues in the food market: a systematic literature review

Gizaw

2019

Environ Health Prev Med

200

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Background: Food safety in the food market is one of the key areas of focus in public health, because it affects people of every age, race, gender, and income level around the world. The local and international food marketing continues to have significant impacts on food safety and health of the public. Food supply chains now cross multiple national borders which increase the internationalization of health risks. This systematic review of literature was, therefore, conducted to identify common public health risks related to food safety issues in the food market. Methods: All published and unpublished quantitative, qualitative, and mixed method studies were searched from electronic databases using a three step searching. Analytical framework was developed using the PICo (population, phenomena of interest, and context) method. The methodological quality of the included studies was assessed using mixed methods appraisal tool (MMAT) version 2018. The included full-text articles were qualitatively analyzed using emergent thematic analysis approach to identify key concepts and coded them into related non-mutually exclusive themes. We then synthesized each theme by comparing the discussion and conclusion of the included articles. Emergent themes were identified based on meticulous and systematic reading. Coding and interpreting the data were refined during analysis.Results: The analysis of 81 full-text articles resulted in seven common public health risks related with food safety in the food market. Microbial contamination of foods, chemical contamination of foods, food adulteration, misuse of food additives, mislabeling, genetically modified foods (GM foods), and outdated foods or foods past their use-by dates were the identified food safety-related public health risks in the food market. Conclusion: This systematic literature review identified common food safety-related public health risks in the food market. The results imply that the local and international food marketing continues to have significant impacts on health of the public. The food market increases internationalization of health risks as the food supply chains cross multiple national borders. Therefore, effective national risk-based food control systems are essential to protect the health and safety of the public. Countries need also assure the safety and quality of their foods entering international trade and ensure that imported foods conform to national requirements.

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PCR-based assessment of shellfish traceability and sustainability in international Mediterranean seafood markets

Cited by 24 publications

References 26 publications

Lab experience with seafood control at the undergraduate level: Cephalopods as a case study

Lab experience with seafood control at the undergraduate level: Cephalopods as a case study

Molecular detection of adulteration in commercial buffalo meat products by multiplex PCR assay

Public health risks related to food safety issues in the food market: a systematic literature review

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