Isolation and characterization of microsatellites in Pacific white shrimp Penaeus (Litopenaeus) vannamei

Cruz, Pedro; Mejı́a-Ruı́z, Humberto; Pérez‐Enríquez, Ricardo; Ibarra, Ana M.

doi:10.1046/j.1471-8286.2002.00211.x

Cited by 10 publications

(16 citation statements)

References 7 publications

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“…The specific loci were selected from the literature as the most highly diverse for this species (Cruz et al 2002(Cruz et al , 2004Valles-Jiménez et al 2005;Machado-Tamayo 2006;Garcia and Alcivar-Waren 2007;Luvesuto et al 2007). We had 6 and 4 % reduction of H o in Amiri-Gomishan (0.479-0.450) and Amiri-Gorgeaj (0.479-0.458) populations that represent a 5.4 and 3.6 % increase in inbreeding, respectively.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity status of Pacific white shrimp (Litopenaeus vannamei) using SSR markers in Iran

2015

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Genetic diversity is vital for the maintenance of genetic pool in cultured shrimps. In order to estimate the current status of genetic diversity in Litopenaeus vannamei shrimp in Iran, as an exotic species, a total of 45 individuals from Amiri and Gorgeaj farms in Jask port of Hormozgan province and one hatchery in Gomishan city of Golestan province, were detected using four microsatellite loci. The number of alleles per locus was 5-10, and the mean effective number of alleles (N e ) across populations and loci ranged from 4.834 to 5.148. The overall mean observed heterozygosity (H o ) ranged from 0.450 to 0.479, which was lower than the expected one (0.789-0.794). There was nothing remarkable about any of the allele frequencies across populations or loci. The mean inbreeding coefficient (F IS ) and pairwise genetic differentiation (F ST ) among populations were 41.6 % and 0.133, respectively. The three studied populations departed from Hardy-Weinberg equilibrium (HWE). Analysis of molecular variance revealed 14 % variability among and 86 % within populations. However, considering departing from HWE and the high F IS and F, the moderate pairwise F ST values, importance of introducing genetically diverse broodstock and monitoring to control inbreeding is discussed.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity status of Pacific white shrimp (Litopenaeus vannamei) using SSR markers in Iran

2015

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“…A similar reduction in allele numbers has been recorded previously for locus Pvan0013. Only two alleles were present at this locus in cultured populations of L. vannamei (Cruz et al . 2002), while a maximum of six alleles have been typed in natural populations (Valles‐Jimenez et al .…”

Section: Discussionmentioning

confidence: 99%

“…Three previously described microsatellite loci have been chosen, TUMXLv 5.38, TUMXLv 8.2 and Pvan 0013 (Cruz, Mejia‐Ruiz, Perez‐Enriquez & Ibarra 2002; Meehan, Xu, Zuniga & Alcivar‐Warren 2003), based on the reproducibility of the amplicons under our laboratory conditions.…”

Section: Methodsmentioning

confidence: 99%

Genetic monitoring of broodstocks of the marine shrimpLitopenaeus vannameiin a closed rearing system in Pernambuco, Brazil

Lima

Santos

Dantas

et al. 2008

Aquaculture Research

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Loss of genetic variability can be detrimental to a population's survival traits and ¢tness. These e¡ects are likely to be maximized in shrimp closed broodstock rearing systems where post-larvae are often produced from crosses of breeders collected from an associated grow-out farm after mass selection. Longtime broodstock management in closed systems is also expected to lead to reduction in or even complete eradication of genetic variability. The present work aimed at monitoring the genetic variability of a Litopenaeus vannamei hatchery in the state of Pernambuco (Brazil), during three successive replacements, using microsatellite markers. No signi¢cant genetic diversity losses have been observed through the values of mean heterozygosity (H o 5 0.460 and H e 5 0.660 in the ¢rst sample; H o 5 0.420 and H e 5 0.620 in the second sample; and H o 5 0.600 and H e 5 0.660 in the third sample). However, some alleles appear to have been lost after three replacements. The diversity level was considered to be high and is comparable to those reported for wild populations, suggesting that the original imported founder stock of Brazilian L. vannamei is likely to have had a high genetic diversity, possibly due to multiple origins.

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“…The characterization of efficient microsatellites from the genome of this shrimp has been claimed as an important tool in the assessment of genetic variation, pedigree for family selection and genetic improvement strategies, ensuring better genetically based production systems (Wolfus et al . 1997; Cruz et al . 2002; Meehan et al .…”

Section: Characterization Of Eight Microsatellite Loci Isolated In LImentioning

confidence: 99%

“…The Pacific white shrimp Litopenaeus vannamei (Penaeidae) is one of the most important marine shrimp species farmed in several parts of the world. The characterization of efficient microsatellites from the genome of this shrimp has been claimed as an important tool in the assessment of genetic variation, pedigree for family selection and genetic improvement strategies, ensuring better genetically based production systems (Wolfus et al 1997;Cruz et al 2002;Meehan et al 2003;Pérez et al 2005). Moreover, hundreds of microsatellites are needed for a well-saturated genetic mapping.…”

mentioning

confidence: 99%

Isolation and characterization of new microsatellite loci in the Pacific white shrimp Litopenaeus vannamei and cross‐species amplification in other penaeid species

Freitas

Jesus

Galetti

2006

Molecular Ecology Notes

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The goal of the present study was to obtain valuable microsatellite markers useful in genetic approaches of the shrimp Litopenaeus vannamei, an important aquaculture species of the world. Eight loci produced suitable polymorphic patterns with allele number ranging from two to 12 and expected heterozygosity from 0.18 to 0.89. Cross‐species amplification was successfully performed in five other penaeid species (Litopenaeus schmitti, Farfantepenaeus brasiliensis, Farfantepenaeus paulensis, Xiphopenaeus kroyeri and Rimapenaeus constrictus), indicating that some these loci can be useful in studies of other related species.

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Isolation and characterization of microsatellites in Pacific white shrimp Penaeus (Litopenaeus) vannamei

Cited by 10 publications

References 7 publications

Genetic diversity status of Pacific white shrimp (Litopenaeus vannamei) using SSR markers in Iran

Genetic diversity status of Pacific white shrimp (Litopenaeus vannamei) using SSR markers in Iran

Genetic monitoring of broodstocks of the marine shrimpLitopenaeus vannameiin a closed rearing system in Pernambuco, Brazil

Isolation and characterization of new microsatellite loci in the Pacific white shrimp Litopenaeus vannamei and cross‐species amplification in other penaeid species

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