Isolation and characterization of new microsatellite loci in the Pacific white shrimp <i>Litopenaeus vannamei</i> and cross‐species amplification in other penaeid species

Freitas, Patrícia D.; Jesus, Célia Maria de; Galetti, Pedro Manoel

doi:10.1111/j.1471-8286.2006.01595.x

Cited by 15 publications

(4 citation statements)

References 12 publications

(11 reference statements)

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“…Four primer sets, Pvan1758 and Pvan1815 (Cruz et al, 2002) along with Lvan1 and Lvan7 (Freitas et al, 2007), were used in a 25 μL reaction volume containing 50-150 ng of DNA, 0.2 mM of each dNTP, 7.5 pmoles μL -1 of each primer, 1.5-2.5 mM MgCl 2 , 1 unit of Taq DNA Polymerase and 1 to 1.25x PCR buffer. The Master Mix (2.5X) kit (Eppendorf) was used for the Lvan1 and Lvan7 loci.…”

Section: Amplification Of Microsatellite Locimentioning

confidence: 99%

“…Microsatellite analyses have been used to assess genetic variation in several penaeid species, such as L. vannamei (Wolfus et al, 1997), Marsupenaeus japonicus (Moore et al, 1999), L. stylirostris (Bierne et al, 2000), Penaeus monodon (Tassanakajon et al, 1998;Brooker et al, 2000;Pongsomboon et al, 2000, Xu et al, 2001, Litopenaeus schmitti (Maggioni et al, 2003;Borrel et al, Genetics and Molecular Biology, 30, 4, 1156-1160(2007 Copyright by the Brazilian Society of Genetics. Printed in Brazil www.sbg.org.br 2004) and Litopenaeus setiferus (Ball and Chapman, 2003) but, however, little is known on the genetics of the Brazilian L. vannamei broodstocks Galetti Jr 2002, 2005;De Francisco and Galetti Jr, 2005;Gonçalves et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

2007

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The culture of the marine shrimp Litopenaeus vannamei has recently boosted the Brazilian shrimp industry. However, it is well known that selection methods based solely on phenotypic characteristics, a reduced number of breeders and the practice of inbreeding may promote a significant raise in the genetic similarity of the captive populations, leading to greater disease susceptibility and impairing both the growth and final size of the shrimps. We used four microsatellite loci to investigate genetic variation in three generations (F 5 , F 6 and F 7 ) of a closed and reared L. vannamei lineage. Although an accentuated heterozygosis deficit was detected, we also observed that the captive propagation of this lineage did not lead to a significant loss of genetic variability over the three generations studied. One possible reason for this is that the breeding conditions of this lineage were good enough to prevent any significant loss of genetic variability. However, three generations may have been insufficient to produce detectable changes in genetic frequencies in the loci studied. Alternatively, the microsatellite loci may have been non-neutral (biased) and related to the conditions in which the shrimps were kept, resulting in a similar allele pool in respect to these four microsatellites over the three generations studied. Any generalizations regarding microsatellite variation in closed shrimp lines may thus be incomplete and should be carefully analyzed.

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Section: Amplification Of Microsatellite Locimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

2007

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“…The penaeid shrimp Litopenaeus vannamei is considered one of the most important marine shrimp aquaculture species (Pérez, Ortiz, Zhinaula, Gonzabay, Calderón & Volckaert 2005). A recent review of the literature on microsatellite markers in Pacific white shrimp has reported approximately 150 polymorphic loci, mostly isolated from anonymous regions of the genome (Freitas, Jesus & Galetti 2007). Although dbEST (expressed sequence tags database) has been used to isolate microsatellites in shrimp species (Wang, Guo, Zhang, Meng, Qiu, Liu & Zhang 2009), it is poorly known in L. vannamei (Pérez et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Characterization and genomic annotation of polymorphic EST-SSR loci in Litopenaeus vannamei shrimp

et al. 2011

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In the present work, EST‐SSR (expressed sequence tag‐simple sequence repeat) loci were obtained by screening 45 000 ESTs from the Pacific white shrimp Litopenaeus vannamei, which was available in a database of the ShEST (Shrimp EST Genome Project) consortium. Fifty‐two of 600 EST‐SSR loci were selected. From this total, 21 EST‐SSRs were polymorphic among 40 individuals and had their gene products ascribed. Two to 20 alleles per locus were detected and the observed heterozygosity ranged from 0.15 to 0.86. Eight loci presented a significant heterozygote deficit after the Bonferroni correction, which was attributed to null alleles. Seven loci were able to have their protein products, molecular functions and biological processes determined. Our results are promising for future studies that relate the levels of these gene polymorphisms with different biological responses to stress in aquaculture.

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“…In several regions of the world, shrimp Litopenaeus vannamei (Boone, 1931) is one of the most widely used species in the production of cultured marine shrimps (Freitas et al 2007), due to its characteristics of high adaptation, rapid growth, and high survival rates to adverse environmental conditions (Briggs et al 2004). Originally from the Eastern Pacific, this species is used by shrimp farmers throughout the Western Atlantic (Tavares 2011).…”

Section: Introductionmentioning

confidence: 99%

Native and non-native species of Litopenaeus Pérez-Farfante, 1969 (Crustacea: Penaeidae) from the East Atlantic: Geometric morphometrics as a tool for taxonomic discrimination

Moraes

Alencar

et al. 2021

An. Acad. Bras. Ciênc.

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The shape of the secondary sexual characters is, traditionally, used to discriminate the marine shrimps at the species level. However, the qualitative evaluation of structures that are morphologically variable in the taxonomy of species can favor misunderstandings and misidentifications. These possibilities of taxonomic inaccuracies are especially alarming when there is a need to evaluate the invasion of introduced species. The present study used geometric morphometric analyses to identify differences in the cephalothorax shape that would help discriminate the native and non-native species of Litopenaeus of the South American coast. The comparative morphology analysis was conducted using the right profile of adult males' cephalothorax of L. schmitti, captured in the natural environment, and L. vannamei captured in the natural environment or grown in shrimp farms. In intraspecific evaluation, it was not possible to distinguish the specimens of L. vannamei that were grown in shrimp farms from those acclimated to the natural environment. However, significant interspecific differences in shape were found in the shape of this body structure. Additionally, the base position of the first rostral spine to the tip of the hepatic spine is indicated as a characteristic that can be used to distinguish these two species by eye in the field.

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Isolation and characterization of new microsatellite loci in the Pacific white shrimp Litopenaeus vannamei and cross‐species amplification in other penaeid species

Cited by 15 publications

References 12 publications

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

Characterization and genomic annotation of polymorphic EST-SSR loci in Litopenaeus vannamei shrimp

Native and non-native species of Litopenaeus Pérez-Farfante, 1969 (Crustacea: Penaeidae) from the East Atlantic: Geometric morphometrics as a tool for taxonomic discrimination

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