Isolation and characterization of microsatellite DNA loci from the golden cuttlefish, Sepia esculenta Hoyle (Cephalopoda)

Abstract

The first microsatellite markers were isolated from the golden cuttlefish, Sepia esculenta Hoyle. Eleven primer sets were designed to amplify the marker sequences via polymerase chain reaction. The 45 -50 individuals from one wild population in the coastal waters of Ehime Prefecture, Japan were used to screen polymorphism in the 11 microsatellite loci. All the microsatellite loci were polymorphic, with the range of alleles from seven to 27 per locus. The observed and expected heterozygosities ranged from 0.380 to 0.980 and from 0.654 to 0.940, respectively. These marker loci except for one locus showing significant deviation from Hardy-Weinberg equilibrium will be useful for the assessment of genetic variation and population structure of this species.

Genetic diversity and population structure of the golden cuttlefish, Sepia esculenta (Cephalopoda: Sepiidae) indicated by microsatellite DNA variations

“…We assessed variation at nine polymorphic Sepia esculenta microsatellite loci: Secu6 , Secu75 , Secu84 , Secu101 , Secu113 , Secu117 , Secu146 , Secu164 and SecuC10 (Zheng et al. 2007).…”

“…2007). PCR primers and amplification conditions of these nine loci were as described previously (Zheng et al. 2007).…”

The contribution of molecular data to our understanding of cephalopod evolution and systematics: a review

“…Shaw and Pérez-Losada 2000;Zheng et al 2001Zheng et al , 2005Zheng et al , 2007Zheng et al , 2009Pérez-Losada et al 2002a, 2007Kassahn et al 2003;Garoia et al 2004;Wolfram et al 2006aWolfram et al , 2006bWu et al 2010), although sometimes using mitochondrial markers (e.g. Turan and Yaglioglu 2010).…”

Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)

“…Twenty four microsatellite loci were tested for the presence of null alleles and the results showed that they might be present in seven loci (J5/J8/J10/J17/J60/J63/J74), five of which deviated from HWE. According to Zheng’ report, a significant deviation from HWE was observed in one locus, and null allele existed in the same locus [ 6 ]. Wang also reported that, significant occurrences of null alleles were found for four of five loci that deviated from HWE [ 8 ].…”

“…Among molecular markers, microsatellite markers have been shown to be an extremely valuable tool for the study of population structure because of their variability, abundance, neutrality, co-dominance and unambiguous scoring of alleles [ 9 , 10 ]. Until now, 31 microsatellite markers have been reported for S. esculenta [ 6 , 8 ]. However, only 25 of these conformed to the Hardy-Weinberg equilibrium (HWE).…”