Isolation and characterization of<i>Salmonella enterica</i>in day-old ducklings in Egypt

Osman, Kamelia M.; Marouf, Sherif; Zolnikov, Tara Rava; Nayerah, AlAtfeehy

doi:10.1179/2047773213y.0000000118

Cited by 27 publications

(17 citation statements)

References 58 publications

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“…The resistance pattern of clindamycin was similar to the observation of Cossi et al who also found all isolates in their study resistant to clindamycin [40]. The results of colistin are in disagreement with Osman et al who found most of their isolates sensitive to colistin while we recorded a higher percentage of resistance [41]. Increase in the trend of colistin resistance has been reported due to mis-sense mutations in two genes, pmrA and pmrB genes, which encode a regulator and sensor of a two-component regulatory system of outer membrane [42].…”

Section: Discussionsupporting

confidence: 77%

Isolation, serotype diversity and antibiogram of Salmonella enterica isolated from different species of poultry in India

Mir

Kashyap

Maherchandani

2015

Asian Pacific Journal of Tropical Biomedicine

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A B S T R A C TObjective: To study the occurrence and serotype diversity of Salmonella isolates in different species of poultry (chicken, emu and duck) and determine their resistance pattern against various antibiotics of different classes. Methods: About 507 samples comprising 202 caecal contents and 305 fecal samples from chicken, emu and duck were processed for isolation of Salmonella enterica. Salmonellae were isolated and detected by standard protocol of ISO 6579 Amendment 1: Annex D. Genetic confirmation was also made by using 16S rRNA genus specific PCR. Serotype specific PCR was also done to detect the most common serovars viz. Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Gallinarum. All obtained isolates were subjected to a set of 25 antibiotics to study their antibiogram by using Baeur-Kirby disk diffusion method. Results: Out of 507 samples processed, 32 isolates of Salmonella enterica (18 from caecal contents and 14 from faecal samples) were obtained, of which 24 belonged to 6 different serovars, 6 were untypeable and 2 were rough strains. Salmonella Enteritidis was the most predominant serotype (9), followed by Salmonella Typhimurium (5), Salmonella Virchow (4), Salmonella Gallinarum (3), Salmonella Reading (2) and Salmonella Altona (1). Antibiotic resistance pattern was maximum (100%) to oxacillin, penicillin and clindamycin, followed by ampicillin (68.75%), tetracycline (65.62%), nalidixic acid (56.25%) and colistin (46.87%). High sensitivity of isolates was recorded for chloramphenicol (96.87%) followed by meropenem (84.37%). Conclusions: Occurrence of high proportion of serovars in our study which can cause serious gastroenteritis in humans is a matter of concern. Salmonella Altona has been detected for the first time in India from poultry. This serotype is known to cause serious outbreaks of gastroenteritis in humans. Multidrug resistant isolates were recovered at high percentage which can be attributed to non-judicious use of antibiotics both in prophylaxis and treatment regimen. This observation draws serious attention as poultry serves as an important source of transmission of these multidrug resistant Salmonella serovars to humans.

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Section: Discussionsupporting

confidence: 77%

Isolation, serotype diversity and antibiogram of Salmonella enterica isolated from different species of poultry in India

Mir

Kashyap

Maherchandani

2015

Asian Pacific Journal of Tropical Biomedicine

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“…Farzan et al, (2010) reported 31.5% prevalence of Salmonella in swine. Pork and beef also play a great role in causing salmonellosis apart from poultry meat (Litrup et al, 2010;Osman et al, 2014;Abatcha et al, 2018).…”

Section: Isolation Identification Confirmation and Serotyping Of Samentioning

confidence: 99%

Isolation and Identification of Salmonella spp. from Broiler Chicken Meat in Sri Lanka and their Antibiotic Resistance

et al. 2020

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Purpose : Salmonella infections continue to be a global problem with millions of humans and animal cases occurring annually. Broiler chicken plays a significant role causing Salmonella infections in Sri Lanka. Consumption of food contaminated with antimicrobial resistant Salmonella aggravates the problem. This study isolated, identified, and serotype the Salmonella spp. from broiler chicken meat in Sri Lanka and examined their antimicrobial susceptibility to be used in establishment of control measures. Research Method : Isolation of Salmonella species from broiler chicken meat was done by conventional method of isolation followed by polymerase chain reaction (PCR) confirmation. All PCR confirmed isolates of Salmonella were serotype and then, isolates were tested for antibiotic susceptibility using disc diffusion assay followed by the detection of antibiotic resistance genes using PCR. Findings : Broiler chicken meat in Sri Lanka is contaminated with Salmonella spp. at the prevalence of 11.6% and 8.9% of them carried hns and invA specific genes. Isolates were serotyped as Salmonella Typhimurium (47.8%), Salmonella Enteritidis (26.1%) and non typable (26.1%). Three isolates were resistant to ampicillin. Intermediate resistance was shown to three antibiotics and all the isolates were sensitive to nine antibiotics. Majority (56.5%) of Salmonella were sensitive to all the tested antibiotics. Prevalence of resistant genes for tetracycline, sulfonamides and aminoglycosides were within 4%-26%. None of the isolates except one (4%) carried chloramphenicol resistance genes. Originality / Value : Steps must be taken to minimize contamination of broiler chicken meat with Salmonella spp in Sri Lanka. Although, there is a low prevalence of antibiotic resistant isolates, its mere presence in broiler chicken is a warning signal of possibility of emergence of multidrug resistant strains.

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“…was recovered from diarrheic neonatal calves [ 40 ]. Infections among imported ducklings and camels were considered as an additional source for salmonellosis in Egypt [ 41 , 42 ].…”

Section: Bacterial Zoonosismentioning

confidence: 99%

A Comprehensive Review of Common Bacterial, Parasitic and Viral Zoonoses at the Human-Animal Interface in Egypt

2017

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Egypt has a unique geographical location connecting the three old-world continents Africa, Asia and Europe. It is the country with the highest population density in the Middle East, Northern Africa and the Mediterranean basin. This review summarizes the prevalence, reservoirs, sources of human infection and control regimes of common bacterial, parasitic and viral zoonoses in animals and humans in Egypt. There is a gap of knowledge conerning the epidemiology of zoonotic diseases at the human-animal interface in different localities in Egypt. Some zoonotic agents are “exotic” for Egypt (e.g., MERS-CoV and Crimean-Congo hemorrhagic fever virus), others are endemic (e.g., Brucellosis, Schistosomiasis and Avian influenza). Transboundary transmission of emerging pathogens from and to Egypt occurred via different routes, mainly importation/exportation of apparently healthy animals or migratory birds. Control of the infectious agents and multidrug resistant bacteria in the veterinary sector is on the frontline for infection control in humans. The implementation of control programs significantly decreased the prevalence of some zoonoses, such as schistosomiasis and fascioliasis, in some localities within the country. Sustainable awareness, education and training targeting groups at high risk (veterinarians, farmers, abattoir workers, nurses, etc.) are important to lessen the burden of zoonotic diseases among Egyptians. There is an urgent need for collaborative surveillance and intervention plans for the control of these diseases in Egypt.

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Isolation and characterization ofSalmonella entericain day-old ducklings in Egypt

Cited by 27 publications

References 58 publications

Isolation, serotype diversity and antibiogram of Salmonella enterica isolated from different species of poultry in India

Isolation, serotype diversity and antibiogram of Salmonella enterica isolated from different species of poultry in India

Isolation and Identification of Salmonella spp. from Broiler Chicken Meat in Sri Lanka and their Antibiotic Resistance

A Comprehensive Review of Common Bacterial, Parasitic and Viral Zoonoses at the Human-Animal Interface in Egypt

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