Isolation and Identification of Salmonella spp. from Broiler Chicken Meat in Sri Lanka and their Antibiotic Resistance

Abstract

Purpose : Salmonella infections continue to be a global problem with millions of humans and animal cases occurring annually. Broiler chicken plays a significant role causing Salmonella infections in Sri Lanka. Consumption of food contaminated with antimicrobial resistant Salmonella aggravates the problem. This study isolated, identified, and serotype the Salmonella spp. from broiler chicken meat in Sri Lanka and examined their antimicrobial susceptibility to be used in establishment of control measures. Research Method : Isolation of Salmonella species from broiler chicken meat was done by conventional method of isolation followed by polymerase chain reaction (PCR) confirmation. All PCR confirmed isolates of Salmonella were serotype and then, isolates were tested for antibiotic susceptibility using disc diffusion assay followed by the detection of antibiotic resistance genes using PCR. Findings : Broiler chicken meat in Sri Lanka is contaminated with Salmonella spp. at the prevalence of 11.6% and 8.9% of them carried hns and invA specific genes. Isolates were serotyped as Salmonella Typhimurium (47.8%), Salmonella Enteritidis (26.1%) and non typable (26.1%). Three isolates were resistant to ampicillin. Intermediate resistance was shown to three antibiotics and all the isolates were sensitive to nine antibiotics. Majority (56.5%) of Salmonella were sensitive to all the tested antibiotics. Prevalence of resistant genes for tetracycline, sulfonamides and aminoglycosides were within 4%-26%. None of the isolates except one (4%) carried chloramphenicol resistance genes. Originality / Value : Steps must be taken to minimize contamination of broiler chicken meat with Salmonella spp in Sri Lanka. Although, there is a low prevalence of antibiotic resistant isolates, its mere presence in broiler chicken is a warning signal of possibility of emergence of multidrug resistant strains.

Isolation, Characterization and Pathogenicity of the Most Common Bacteria Associated with Gut Health in Egyptian Broiler Chicken Flocks

“…This study showed isolation of Salmonellae in percentage of 11 (11/100). Similar isolation rates were previously reported by Kaoud et al (2018) and Soliman et al (2018) who isolated Salmonellae from the Egyptian broiler flocks in rates of 11.33 % and 10.37%, respectively, Moreover, in Seri Lanka, Jayaweera et al (2020) demonstrated isolation of Salmonellae from chickens in rate of 11.6%. However, Egyptian studies of Sedeik et al (2019) and El-Sheikh et al (2019) showed isolation of Salmonellae from broiler chicks in rates of 7.5% and 16%, respectively.…”

Antibiotic resistance and virulence genes profile of Non typhodial Salmonella species isolated from poultry enteritis in India

“…spvC, cdtB and sefA were not detected in any isolates.Further, invA, hilA, sivH, tolC and cbtB genes have an important role in host cell invasion and presence of these genes is indicative of invasive properties of eld isolates(Ilyas et al, 2017). Our ndings are in accordance with Awad, et al, (2020) andJayaweera, et al, (2020) who reported the presence of invA, hilA, sivH, tolC and cbtB genes in eld isolates from various sources. Our ndings about invA gene are also in agreement with previous report of (Abdelfatah et al, 2018).…”

Occurrence, Antibiotic Susceptibility and Genes Encoding Antibacterial Resistance of Salmonella spp. and Escherichia coli From Milk and Meat Sold in Markets of Bushenyi District, Uganda

“…Salmonella spp., and E. coli were previously reported in food specimen by various investigators from both West, East, North and Southern African countries. 26 - 30 Such observation implies that these members of Enterobacteriaceae infecting potential pathogens thrive within the environment in the region. It also indicates that the food specimen both harbours and serve as carrier/distributor of these potential pathogens.…”