Isolation and Characterization of Highly Potent Mosquitocidal Bacilli from Egyptian Environment

Foda, M. S.; Amin, Magdi; El-Tayeb, Osama M.; Gawdat, Noha A; El-Bendary, Magda A.

doi:10.3923/jbs.2013.483.490

Cited by 3 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Egyptian isolate Ls 9B24 was isolated from Alexandria governorate and showed high larvicidal potency against C. pipiens (Foda, Amin, et al, 2013). The international strain Ls 2362 was obtained from Prof. Dr Fergus G. Priest, HeriotWatt University, UK.…”

Section: Micro-organisms and Inoculum Preparationmentioning

confidence: 99%

Economic production ofLysinibacillus sphaericusunder solid state fermentation

Foda

Amin

Gawdat

et al. 2015

Biocontrol Science and Technology

Self Cite

View full text Add to dashboard Cite

A highly active mosquitocidal Lysinibacillus sphaericus namely Ls 9B24 was isolated from soil of Alexandria governorate in Egypt. It was more active than the standard strain, L. sphaericus 2362. The sporulation and toxin formation of both cultures grown on different leguminous seeds and by-products under solid state fermentation (SSF) were studied. Among the tested substrates, 6% cotton seed meal enhanced sporulation and the mosquitocidal activity of L. sphaericus 2362, while 6% fodder yeast enhanced sporulation and the mosquitocidal activity of Ls 9B24. The optimum SSF growth conditions for maximum mosquitocidal activity by both cultures were using coarse wheat bran as a carrier material, 50% initial moisture content, 4-64 × 10 6 colony forming units (CFU)/g solid medium inoculum and 6 days' incubation period at 30°C. Addition of 0.5% yeast extract enhanced toxicity about 2.2 and 1.8 fold for L. sphaericus 2362 and Ls 9B24, respectively.

show abstract

Section: Micro-organisms and Inoculum Preparationmentioning

confidence: 99%

Economic production ofLysinibacillus sphaericusunder solid state fermentation

Foda

Amin

Gawdat

et al. 2015

Biocontrol Science and Technology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Egyptian strain, L. sphaericus 14N1, (El-Bendary et al, 2003) and the standard strain of Bti are used in this study. Bacillus thuringiensis israelensis was obtained from Prof. Dr. Fergus G. Priest, Heriot-Watt University, United Kingdom.…”

Section: Microorganismsmentioning

confidence: 99%

Downstream processing of mosquitocidal toxins from solid state fermentation of Lysinibacillus sphaericus and Bacillus thuringiensis israelensis

El-Bendary¹,

Moharam²,

Hamed³

et al. 2017

MJM

View full text Add to dashboard Cite

Aims:The aim of this study was downstream processing of moquitocidal toxins produced by Lysinibacillus sphaericus (L. sphaericus) and Bacillus thuringiensis israelensis (Bti) under solid state fermentation. Methodology and results: Two mosquitocidal strains (L. sphaericus and Bti) were grown separately in trays under solid state fermentation for toxin production. The best conditions for extraction of crude toxins from fermented solids of both cultures were tap water at 5-50 °C, for 10 min under static conditions. Also, concentrated mosquitocidal toxins were efficiently extracted from fermented solids by 4 constitutive additions of 500 mL tap water to 1 kg of fermented culture at room temperature (25 °C) for 5 min each under static conditions. Both extracted toxins were formulated with talcum powder and they were stable for 8 months at room temperature. Conclusion, significance and impact of study: It is very important to study the operating conditions for mosquitocidal toxins extraction from solid state fermentation (SSF) and its formulation in cost effective manner.

show abstract

Molecular Characterization and Evaluation of Two Potential Mosquitocidal Lysinibacillus Strains from Himalayan Valley Kashmir

Lone¹,

Arulselvi²

2017

J PURE APPL MICROBIO

View full text Add to dashboard Cite

Isolation and Characterization of Highly Potent Mosquitocidal Bacilli from Egyptian Environment

Cited by 3 publications

References 17 publications

Economic production ofLysinibacillus sphaericusunder solid state fermentation

Economic production ofLysinibacillus sphaericusunder solid state fermentation

Downstream processing of mosquitocidal toxins from solid state fermentation of Lysinibacillus sphaericus and Bacillus thuringiensis israelensis

Molecular Characterization and Evaluation of Two Potential Mosquitocidal Lysinibacillus Strains from Himalayan Valley Kashmir

Contact Info

Product

Resources

About