Isolation and Characterization of Fish-Gut Bacillus spp. as Source of Natural Antimicrobial Compounds to Fight Aquaculture Bacterial Diseases

Santos, Rafaela; Oliva-Teles, Aires; Pousão-Ferreira, Pedro; Jerusik, Russell; Saavedra, María José; Enes, Paula; Serra, Cláudia R.

doi:10.1007/s10126-021-10022-x

Cited by 25 publications

(25 citation statements)

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“…In addition to their well-known antimicrobial activity, Bacillus spp. are also producers of quorum-quenching (QQ) molecules [27,29,41]. The disruption of pathogens' communication or quorum-sensing via QQ molecules has been proposed as a promising alternative to fighting bacterial infections in aquaculture [15], which remains a major constraint to the sustainable development of the sector.…”

Section: Discussionmentioning

confidence: 99%

“…isolates (FI) as a source of QQ molecules [27]. In this study, we took advantage of 200 FI isolated from the gut of different fish species (Sparus aurata, Dicentrarchus labrax, and Diplodus sargus) [24,27] to further explore their QQ potential, by testing their ability to partially or completely degrade AHLs' QS molecules used by gram-negative fish pathogens. Consistent with the literature, the results indicate that fish gut Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial strains used in this study are listed in Table 3. Fish-Isolates (FI) from our collection obtained from the gut of different fish species (Sparus aurata, Dicentrarchus labrax, and Diplodus sargus) [24,27], and the laboratory strain Bacillus subtilis 168 [77] used as control for FI growth, were routinely and aerobically grown in Luria-Bertani (LB; Fisher BioReagents, Waltham, MA, U.S.A) medium at 37 • C. QQ biosensor strains, Chromobacterium violaceum (CECT 494, ATCC 12472) and Chr. violaceum CV026 (CECT 5999) (Table 1), were grown aerobically in LB medium at 30 • C. Chr.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

“…Bacillus spp. are spore-forming, gram-positive bacteria with recognizable biotechnology applications, including as probiotic-health-promoting agents or as a source of antimicrobial molecules with the capacity to inhibit fish pathogens [21][22][23][24][25][26][27]. Indeed, from the gut of marine fish species, we recently isolated several Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, from the gut of marine fish species, we recently isolated several Bacillus spp. strains capable of inhibiting the growth and biofilm formation of 14 different fish pathogens with in vitro QQ capacity [27]. Recent reports suggest that Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

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Bacillus spp. Inhibit Edwardsiella tarda Quorum-Sensing and Fish Infection

et al. 2021

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The disruption of pathogen communication or quorum-sensing (QS) via quorum-quenching (QQ) molecules has been proposed as a promising strategy to fight bacterial infections. Bacillus spp. have recognizable biotechnology applications, namely as probiotic health-promoting agents or as a source of natural antimicrobial molecules, including QQ molecules. This study characterized the QQ potential of 200 Bacillus spp., isolated from the gut of different aquaculture fish species, to suppress fish pathogens QS. Approximately 12% of the tested Bacillus spp. fish isolates (FI). were able to interfere with synthetic QS molecules. Ten isolates were further selected as producers of extracellular QQ-molecules and their QQ capacity was evaluated against the QS of important aquaculture bacterial pathogens, namely Aeromonas spp., Vibrio spp., Photobacterium damselae, Edwardsiela tarda, and Shigella sonnei. The results revealed that A. veronii and E. tarda produce QS molecules that are detectable by the Chr. violaceum biosensor, and which were degraded when exposed to the extracellular extracts of three FI isolates. Moreover, the same isolates, identified as B. subtilis, B. vezelensis, and B. pumilus, significantly reduced the pathogenicity of E. tarda in zebrafish larvae, increasing its survival by 50%. Taken together, these results identified three Bacillus spp. capable of extracellularly quenching aquaculture pathogen communication, and thus become a promising source of bioactive molecules for use in the biocontrol of aquaculture bacterial diseases.

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