Isolation and Characterization of Enterococci from Yoghurts of Bangladesh

Tabrejee, Shamira; Chowdhury, Miraj Kobad; Bari, Latiful; Yeasmin, Sabina

doi:10.5812/modernc.74460

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…The catalase test or the decomposition of H 2 O 2 to generate O 2 is one of the most useful diagnostic tests for detecting bacteria due to its simplicity. It is well known that Lactobacillus is catalase negative as similar results were found in many studiesof yoghurt samplessuch as the study of isolation and characterization of Enterococci from Yoghurts of Bangladesh by Tabrejee et al, (Tabrejee et al, 2018).The isolates were grown on Simmon's citrate agar slant test tube for the identi cation of acid or alkali-producing bacteria. From the Simmon's citrate test we observed that, all the samples showed negative results.…”

Section: Discussionmentioning

confidence: 62%

Isolation, characterization, and evaluation of probiotic properties of Lactic Acid bacteria from different fermented yoghurt drinks available in Bangladesh

Akter¹,

Jabin²,

Yeasmin³

et al. 2023

Preprint

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Lactobacillus spp. is the most commonly found probiotics strain in dairy products. In the present study, Lactobacillus spp. were isolated from four commercially available fermented yoghurt drinks, which were identified based on observing their morphological characteristics and through some biochemical tests such as, gram staining, MIU (Motility Indole Urease), catalase, citrate, TSI (Tripplr Sugar Iron), gas from glucose, milk coagulation activities, starch hydrolysis and bile esculin test.It was observed that isolated Lactobacillus spp. growth rate decreased with the increasing concentration of inhibitory substances like NaCl (2,4,8%) and phenol (0.1-0.8%). Additionally, good growth were observed in the presence of 2% NaCl and 0.1% phenol. The isolated Lactobacillus spp. did show good survival abilities in acidic (pH 2) and alkaline (pH 8) conditions, while their maximum growth was observed at pH 8.0. Isolated Lactobacilli were able to coagulate skim milk and produce gas from glucose.All of the isolates showed negative results in hemolytic activity, indicating that lactic acid bacteria are safe for use by humans. All isolates were evaluated for their antimicrobial activity against Staphylococcus aureus, Enterococcusspp, Bacillus spp, Escherichia coli, Salmonella typhi, Klebsiellaspp, Pseudomonas spp, Serratia spp, Candida albicans, Trichodermaresseii, Aspergillus spp. The majority of the isolates showed significant antimicrobial effectiveness against the targeted pathogens.Their susceptibility to selected eight antibiotics was determined in terms of zone of inhibition (mm). In conclusion, most of the results from the present experiments showed that, there were very few variations in probiotics properties of the isolated Lactobacillus spp. from different fermented yoghurt drinks.

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Section: Discussionmentioning

confidence: 62%

Isolation, characterization, and evaluation of probiotic properties of Lactic Acid bacteria from different fermented yoghurt drinks available in Bangladesh

Akter¹,

Jabin²,

Yeasmin³

et al. 2023

Preprint

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show abstract

“…For molecular characterization and identification of species, partial sequences of 18S rDNA was amplified using the primers: Forward 5′-AGGGC AAGTC TGGTG CCAGCAG-3′ and Reverse 5′-GTTGA GTCAA ATTAA GCCGC-3′. Genomic DNA was extracted using modified phenol-chloroform-isoamyl alcohol method (Tabrejee et al, 2018). Briefly, individual algal culture was rinsed with distilled water followed by with 70% ethanol.…”

Section: Dna Isolation and Pcrmentioning

confidence: 99%

Molecular characterization and new reports of two green algae from Bangladesh

Alfasane¹,

Chowdhury²,

Mehnaz³

2019

Bangladesh J. Plant Taxon

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This communication portrays the molecular characterization and confirms the new reports of two fresh water green algae namely, Pithophora polymorpha Wittrock and Spirogyra maxima (Hassall) Wittrock from Bangladesh. The samples of these algal species were cultured and partial 18S rDNA was sequenced and analysed for their molecular identification. It was found that the primers reported here could sufficiently identify these algae as P. polymorpha and S. maxima. Furthermore, the Neighbour joining (NJ) tree generated from 18s rDNA sequences suggested that Spirogyra maxima of Bangladesh is distantly related to the cluster of S. juergensii and S. platensis. Pithophora polymorpha along with P. roettleri, P. sano and Pithophora sp. seems to form a strongly supported monophyletic group. The alga AP1 clusters with Pithophora and the alga AS1 clusters with Spirogyra. This study is the first-time report of molecular identification of Bangladeshi algae and a landmark towards the future exploration of thealgal biodiversity of Bangladesh.

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Isolation and Characterization of Enterococci from Yoghurts of Bangladesh

Cited by 2 publications

References 15 publications

Isolation, characterization, and evaluation of probiotic properties of Lactic Acid bacteria from different fermented yoghurt drinks available in Bangladesh

Isolation, characterization, and evaluation of probiotic properties of Lactic Acid bacteria from different fermented yoghurt drinks available in Bangladesh

Molecular characterization and new reports of two green algae from Bangladesh

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