Objectives::
This investigation aims to isolate the Lactic Acid Bacteria (LAB) from yogurt
samples from the Tangail district of Bangladesh and evaluate their probiotic properties.
background:
Usual techniques of microbiological and biochemical process were used to identify them.
Methods::
A rigorous study was carried out from April to August, 2019. A total of 10 samples
were examined by following the standard bacteriological methods. Besides the biochemical and
antibiogram tests, molecular characterization of the isolated bacteria was carried out.
Results::
Ten isolates were confirmed as LAB, and they exhibited excellent probiotic properties.
They were able to survive in both acidic (pH 2) and alkaline (pH 8) conditions. They also survived
against phenol (0.1-0.4%) and NaCl (1-10%) after 24 h incubation. Most of the isolates harboured
plasmid DNA and showed good resistance patterns against the antibiotics used. During the
mating experiment, no X-conjugants were found.
Conclusion::
In conclusion, the mating experiment suggested that the acquired resistant genes
might be chromosome mediated, and isolates showed good probiotic properties that confer health
benefits.