Isolation and characterization of dextran produced by Lactobacillus sakei L3 from Hubei sausage

Wang, Binbin; Song, Qiaozhi; Zhao, Fangkun; Zhang, Lixia; Han, Yongdian

doi:10.1016/j.carbpol.2019.115111

Cited by 45 publications

(25 citation statements)

References 45 publications

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“…The maximum height of levan was much higher than the height of a single polysaccharide chain(0.1-1nm) suggesting that the tightly packed molecular structure formed in AFM images may be caused by the intermolecular and intramolecular aggregation of levan (Sun et al 2018). A similar result was reported for the EPS polymer from Lactobacillus sakei L3 (Wang et al 2019b) but different from Lactobacillus reuteri E81 glucan which had the tangled networks (İspirli et al 2019) and Mesona blumes gum EPS polymer which had an irregular shape like the worm (Tao et al 2008).…”

Section: Afm Analysissupporting

confidence: 75%

Biosynthesis and Structural Characterization of Levan by a Recombinant Levansucrase From Bacillus Subtilis ZW019

Wang

Zhao

et al. 2021

Preprint

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ObjectivesThe yield of levan extracted from microbial fermentation broth is low, so in vitro catalytic synthesis of levan by levansucrase is expected to be one of the industrial production approaches of levan. ResultsA recombinant plasmid Pet-28A-AcmA-Z constructed in the previous study was used to produce levansucrase. The recombinant levansucrase could be easily purified in one step and the purified enzyme had a single band clearly visible in SDS-PAGE. The conditions for enzymatic reactions was optimal at pH 5.2 and 40 ℃, and the activity of enzymes was stimulated by K+ and Ca2+. The yield of levan biosynthesis from 10% (w/v) sucrose with 6.45 U/g sucrose of levansucrase was 30.6 g/L. The molecular weight of the levan was about 1.56×106 Da, as measured by GPC. HPIC analysis showed that the monosaccharide composition of the levan was fructose and glucose. The results of FTIR and NMR analysis indicated that the polymer produced by the recombinant levansucrase was β-(2, 6) levan.ConclusionsThe results of this study provide a basis for large-scale production of levan by enzymatic method.

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Section: Afm Analysissupporting

confidence: 75%

Biosynthesis and Structural Characterization of Levan by a Recombinant Levansucrase From Bacillus Subtilis ZW019

Wang

Zhao

et al. 2021

Preprint

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“…plantarum WLPL04 is comprised of xylose, glucose and galactose [29], whereas the EPS of Lactococcus lactis SLT10 is comprised of glucose, mannose and rhamnose [30]. The EPS of Lactobacillus sakei L3 has a dextran containing d -glucose residues with α−1, 6 glycosidic linkage, which has high viscosity at high concentration, low temperature and acidic pH [31]. Further studies are needed on the EPS structure and viscosity of Lact.…”

Section: Resultsmentioning

confidence: 99%

Lactic acid bacterial exopolysaccharides strongly bind histamine and can potentially be used to remove histamine contamination in food

et al. 2021

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The symptoms of foodborne histamine poisoning are similar to those of IgE-mediated food allergies. In this study, we investigated the histamine-binding capacity of lactic acid bacteria (LAB) strains as potential preventive agents against histamine poisoning. Histamine biosorption capacity was determined for 16 LAB strains. Leuconostoc mesenteroides TOKAI 51 m, Lactobacillus paracasei TOKAI 65 m, Lactobacillus plantarum TOKAI 111 m and Pediococcus pentosaceus TOKAI 759 m showed especially high biosorption rates and reached saturation within 30 min. Adsorption isotherms showed better conformance to the Freundlich model than to the Langmuir model. Analyses after heat, periodic acid and guanidine hydrochloride treatments suggested that histamine was bound to the bacterial cell surface. HPLC analysis revealed that exopolysaccharides produced by Lact. paracasei TOKAI 65 m strongly bound to histamine. In the detachment test with 1 mol l−1 HCl solution, the dissociation rate of histamine for Lact. paracasei TOKAI 65 m was <10 %. This strain is presumably a suitable candidate for use against histamine poisoning.

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“…Dextran is soluble in ethylene glycol, formamide, glycerol, methyl sulfoxide, and water [17]. The morphology of freeze-dried dextran can be either porous or non-porous, depending on the microbial strain [83]. The morphology of dextran influences its ability to hold water and form a gel.…”

Section: Dextranmentioning

confidence: 99%

“…Dextran is synthesized by lactic acid bacteria (LAB) belonging to the Weisella, Lactobacillus, Pediococcus, and Leuconostoc genera in a sucrose-rich media [83,[85][86]. The molecular weight and the yield of dextran production depend on the process parameters, namely, the carbon concentration, temperature, and the microbial strain [87].…”

Section: Dextranmentioning

confidence: 99%

Biomedical Applications of Bacterial Exopolysaccharides: A Review

et al. 2021

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Bacterial exopolysaccharides (EPSs) are an essential group of compounds secreted by bacteria. These versatile EPSs are utilized individually or in combination with different materials for a broad range of biomedical field functions. The various applications can be explained by the vast number of derivatives with useful properties that can be controlled. This review offers insight on the current research trend of nine commonly used EPSs, their biosynthesis pathways, their characteristics, and the biomedical applications of these relevant bioproducts.

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Isolation and characterization of dextran produced by Lactobacillus sakei L3 from Hubei sausage

Cited by 45 publications

References 45 publications

Biosynthesis and Structural Characterization of Levan by a Recombinant Levansucrase From Bacillus Subtilis ZW019

Biosynthesis and Structural Characterization of Levan by a Recombinant Levansucrase From Bacillus Subtilis ZW019

Lactic acid bacterial exopolysaccharides strongly bind histamine and can potentially be used to remove histamine contamination in food

Biomedical Applications of Bacterial Exopolysaccharides: A Review

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